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Development of mesenchymal stem cell engineering for reconstructive therapy

Research Project

Project/Area Number 15208034
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNagoya University

Principal Investigator

KITAGAWA Yasuo  Nagoya Univ., Grad. Sch. Bioagric. Sci., Professor, 大学院・生命農学研究科, 教授 (50101168)

Co-Investigator(Kenkyū-buntansha) TORII Shuhei  Nagoya Univ., Grad. Sch. Medicine., Professor, 大学院・医学系研究科, 教授 (60115607)
鳥山 和宏  あいち小児保健医療センター, 医長
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥52,260,000 (Direct Cost: ¥40,200,000、Indirect Cost: ¥12,060,000)
Fiscal Year 2005: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2004: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2003: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Keywordsadipose tissues / age-dependency / autologous stem cells / fibroblast growth factor / humanized culture / low-serum medium / mesenchym / stromal vascular fraction / 幹細胞 / 幹細胞工学 / 幹細胞保育因子 / 再生医療 / 脂肪由来幹細胞 / 体性幹細胞 / 天井培養 / 間葉系幹細胞 / 成体幹細胞保育因子 / ラミニン / 形成外科 / 自家移植 / 末梢組織 / 大網 / ヒト血清培養 / 骨形成
Research Abstract

Adipose tissues are abundant deposition sites of autologous stem cells with potential application for regenerative medicine and adipose-derived stromal vascular fraction (SVF) is a source of autologous stem cells with differentiation potential along mesenchymal, hematopoietic, neurogenic, angiogenic or epithelial lineage. By culturing SVF cells in high- or low- serum medium containing fibroblast growth factor (FGF)-2, we found that the cells grew faster in low-serum medium and could be subcultured unlimitedly. Cells expanded in low-serum medium showed higher potential of mesenchymal differentiation. We suggest that high-serum concentration is unfavorable for self-renewing of stem cells due to growth factors contained in the serum. When SVF cells from patients at different ages were culture in high-serum medium, stem cells with osteogenic potential were depleted at ages above 20 years, whereas they could be expanded in low-serum medium even from 82 years old patient. Since human adult serum could substitute for 2% fetal bovine serum to give even better proliferation and differentiation, we could humanized ex vivo expansion culture of human stem cells for immediate clinical applications. By our method, autologous stem cell population corresponding to 10^9 cells can be prepared within 3 weeks after obtaining 1 g of subcutaneous adipose tissue and 200 ml of blood from patients at any age.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (26 results)

All 2006 2005 2004 Other

All Journal Article (17 results) Patent(Industrial Property Rights) (3 results) Publications (6 results)

  • [Journal Article] 脂肪組織からの体性幹細胞の分離2006

    • Author(s)
      北川泰雄, 他
    • Journal Title

      The Lipid 17・1

      Pages: 69-75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ヒト脂肪組織由来幹細胞発見の経緯と臨床応用について2006

    • Author(s)
      北川泰雄, 他
    • Journal Title

      日本形成外科学会誌 (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation of adult stem cells from adipose tissues. (in Japanese)2006

    • Author(s)
      Kitagawa Y., Kobori M., Toriyama K., Torii S.
    • Journal Title

      Lipids 17(1)

      Pages: 69-75

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 脂肪組織からの体性幹細胞の分離2006

    • Author(s)
      北川泰雄, 小堀雅登, 鳥山和宏, 鳥居修平
    • Journal Title

      The Lipid 17・1

      Pages: 69-75

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue.2005

    • Author(s)
      Miyazaki Takamichi, et al.
    • Journal Title

      Differentiation 73・2-3

      Pages: 69-78

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      Koparal, Tansu A., et al.
    • Journal Title

      Cytotechmology 46・1

      Pages: 25-36

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue2005

    • Author(s)
      Miyazaki T., Kitagawa Y., Toriyama K., Kobori M., Torii S.
    • Journal Title

      Differentiation 73(2-3)

      Pages: 69-78

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      Koparal, T.A., Yamaguchi, H., Omae, K., Torii, S., Kitagawa, Y.
    • Journal Title

      Cytotechmology 46(1)

      Pages: 25-36

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal dierentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue.2005

    • Author(s)
      Miyazaki T, Kitagawa Y, Toriyama K, Kobori M, Torii S.
    • Journal Title

      Differentiation 73・2-3

      Pages: 69-78

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      Koparal.T.A., Yamaguchi, H., Omae, K., Torii, S., Kitagawa, Y
    • Journal Title

      Cytotechmology 46・1

      Pages: 25-36

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Isolation of two human fibroblastic cell populations with multiple but distinct potential of mesenchymal differentiation by ceiling culture of mature fat cells from subcutaneous adipose tissue.2005

    • Author(s)
      Takamichi Miyazaki, Kazuhiro Toriyama, Masato Kobori, Shuhei Torii, Yasuo Kitagawa1
    • Journal Title

      Differentiation (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential effect of green tea catechins on three endothelial cell clones isolated from rat adipose tissue and on human umbilical vein endothelial cells.2005

    • Author(s)
      A.Tansu Koparal, Hirotake Yamaguchi, Kaoru Omae, Shuhei Torii, Yasuo Kitagawa
    • Journal Title

      Cytotechnology (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Heparin binds to the laminin alpha4 chain LG4 domain at a site different from that found for other laminins.2004

    • Author(s)
      Hironobu Yamashita, Konrad Beck K, Yasuo Kitagawa
    • Journal Title

      Journal of Molecular Biology 335(5)

      Pages: 1145-1149

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Mammalian and Drosophila cells adhere to the laminin alpha4 LG4 domain through syndecans, but not glypicans. 2004 Sep 15;382(Pt3):933-432004

    • Author(s)
      Hironobu Yamashita, Akira Goto, Tasuhiko Kadowaki, Yasuo Kitagawa
    • Journal Title

      Biochem Journal 382(3)

      Pages: 933-943

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Application of RNA interference to chicken embryos using small interfering RNA.2004

    • Author(s)
      Fuminori Sato, Tetsuto Nakagawa, Makoto Ito, Yasuo Kitagawa, Masa-aki Hattori
    • Journal Title

      Experimental Zoolog Part A Comparative Experimental Biology 301(1)

      Pages: 820-827

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Syndecan-dependent binding of Drosophila hemocytes to laminin alpha3/5 chain LG4-5 modules : potential role in sessile hemocyte islets formation2004

    • Author(s)
      Ryo Narita, Hironobu Yamashita, Akira Goto, Hiromi Imai, Shugeyuki Ichihara, Hitoshi Mori, Yasuo Kitagawa
    • Journal Title

      FEBS Lrtters 576・1-2

      Pages: 127-132

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Discovery of adipose-derived stem cells and their clinical application (in Japanese)

    • Author(s)
      Kitagawa Y., Kobori M., Toriyama K., Torii S., Kamei Y.
    • Journal Title

      Journal of Japanese Society for Plastic and Reconstructive Surgery. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 低血清培地で選択的に増殖する動物組織偏在性の多分化性細胞2005

    • Inventor(s)
      北川泰雄 他7名
    • Industrial Property Rights Holder
      科学技術振興機構, 他3者
    • Filing Date
      2005-07-08
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 低血清培地で選択的に増殖する動物組織遍在性の多分化性細胞2005

    • Inventor(s)
      北川 泰雄他7名
    • Industrial Property Rights Holder
      科学技術新興機構他3者
    • Filing Date
      2005-07-08
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] 低血清培養で選択的に増殖する動物組織遍在性の分化多能性細胞2004

    • Inventor(s)
      北川泰雄, 鳥居修平, 桜田一洋, 小堀雅登, 亀井譲, 山口弘毅, 鳥山和宏, 高田徹
    • Industrial Property Rights Holder
      科学技術振興機構, 協和発酵工業殊式会社, 北川泰雄, 鳥居修平
    • Industrial Property Number
      2004-201615
    • Filing Date
      2004-07-08
    • Related Report
      2004 Annual Research Report
  • [Publications] Tajima R, Toriyama K, Sano R, Inou K, Oda H, Torii S, Kitagawa Y: "Reversible heat-denaturation of Matrigel retains biological activity inducing differentiated phenotype of primary cultured hepatocytes and inducing de novo adipogenesis."Tissue Engineering. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yamashita H, Beck K, Kitagawa Y.: "Heparin binds to the laminin α4 chain LG4 domain at a site different from that found for other laminins."Journal of Molecular Biology. 335(5). 1145-1149 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Goto A, Kadowaki T, Kitagawa Y: "Drosophila hemolectin gene is expressed in embryonic and larval hemocytes and its knock down causes bleeding defects"Developmental Biology. 264(2). 582-591 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Suzuki N, Ichikawa N, Kasai S, Yamada M, Nishi N, Morioka H, Yamashita H, Kitagawa Y, Utani A, Hoffman MP, Nomizu M: "Syndecan binding sites in the laminin α1 chain G domain"Biochemistry. 42(43). 12625-12633 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka K, Kitagawa Y, Kadowaki T.: "Misexpression of mouse porcupine isoforms modulates the differentiation of P19 embryonic carcinoma cells"Cell Biology International. 27(7). 549-557 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsushima Y, Matsumura K, Ishii S, Inagaki H, Suzuki T, Matsuda Y, Beck K, Kitagawa Y.: "Functional domains of chicken mitochondrial transcription factor A for the maintenance of mitochondrial DNA copy number in lymphoma cell line DT40."Journal of Biological Chemistry. 278(33). 31149-31158 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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