| Budget Amount *help |
¥50,440,000 (Direct Cost: ¥38,800,000、Indirect Cost: ¥11,640,000)
Fiscal Year 2005: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2004: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2003: ¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
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| Research Abstract |
In this research project, we revealed the followings on GGAs and its related Tom1 family proteins.
(1)We found that ARFs bind to the N-GAT subdomain of the GAT domain of GGAs.
(2)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain in complex with ARF.
(3)We found that ubiquitin bind to the C-GAT subdomain of the GAT domain of GGAs and Tom1 family proteins.
(4)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain and that of the Tom1-GAT domain in complex with ubiquitin.
(5)We found that Tollip (Toll-interacting protein) interacts with the GAT domain of the Tom1 family proteins.
(6)We revealed that Tollip recruits Tom1 family proteins and ubiquitinated proteins onto endosomes.
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