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New classification of digestive system cancers based on gene expression analysis.

Research Project

Project/Area Number 15209044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionKinki University

Principal Investigator

SHIOZAKI Hitoshi  Kinki University, Sch.of Med., Prof., 医学部, 教授 (70144475)

Co-Investigator(Kenkyū-buntansha) OKUNO Kiyotaka  Kinki University, Sch.of Med., Prof., 医学部, 教授 (30169239)
IMAMOTO Haruhiko  Kinki University, Sch.of Med., Assoc.Prof., 医学部, 助教授 (80351609)
HIDA Jin-ichi  Kinki University, Sch.of Med., Assist.Prof., 医学部, 講師 (20238306)
西村 訓弘  (株)ラボ, 主任研究員
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥22,230,000 (Direct Cost: ¥17,100,000、Indirect Cost: ¥5,130,000)
Fiscal Year 2005: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2004: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2003: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
KeywordscDNA array / gene expression analysis / neural network / colorectal cancer / liver metastasis / 食道癌 / 大腸癌肝転移 / ニューラルネットワーク解析
Research Abstract

Gene expression analysis with total RNA extracted from colon and rectal cancer was performed by using of cDNA filter arrays spotted with 1,300 genes relating to cancer and immunity. The number of samples was 51 including six samples overlapped. This study was aimed at the establishment of a new molecular-level prognosis method. We investigated following two points, that is, 1) establishment of prognosis method by constructing the liver metastasis predicting model based on gene expression profiling and 2) extraction of a group of genes relating to liver metastasis. We had chosen a neural network method to build a classifier and a genetic algorithm for its optimization, and then developed the system based on the method with our partner, MediBIC Co.Ltd. (Tokyo). The accuracy of our system was verified by using of the public data, published in Nature Medicine. As a result, an average rate of correct answer was 97.8%. These results suggest that our system and model were preferable for predi … More cting the possibility of metastasis. Then, we applied the system to the construction of liver metastasis forecasting model by using of clinical data. The accuracy of the system was sufficient level also in our case regarding the liver metastasis. Major molecules previously reported to relate to the liver metastases had not unfortunately found in the list of genes extracted based on our model. However, there is still a possibility to find a novel biomarker by investigating the extracted genes further. Because we recognized problems on the total RNA sample preparation, we have left it open to be investigated whether the biomarker candidates are included in the gene list. In this research, we were focusing on the classifier discriminating whether the liver metastasis occurs or not. We are aware however that liver metastasis should to be analyzed by separating into two groups, one is synchronous metastasis and the other is metachronous metastasis. We have started a series of study taking triple classifier approaches, namely, synchronous liver metastasis (5samples), metachronous liver metastasis (5samples) and liver metastasis free (5samples). We are checking the quality of all samples that will be used in the series of the study, at present. Less

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (32 results)

All 2005 2004 Other

All Journal Article (22 results) Book (3 results) Publications (7 results)

  • [Journal Article] Optical ligation level of the primary feeding artery and bowel resection margin in colon cancer surgery.2005

    • Author(s)
      J.Hida, K.Okubo, M.yasutomi, H.Shiozaki, et al.
    • Journal Title

      Dis. Colon Rectum 48 (12)

      Pages: 2232-2237

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Number versus distribution in classifying regional lymph node metastases from colon cancer.2005

    • Author(s)
      J.Hida, K.Okubo, M.yasutomi, H.Shiozaki, et al.
    • Journal Title

      J. Am. Coll. Surg. 201

      Pages: 217-222

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Is preoperative methylprednisolone benefical for patients undergoing esophagectomy?2005

    • Author(s)
      M.Yano, M.Taniguchi, T.Tsuzinaka, H.Shiozaki, et al.
    • Journal Title

      Hepato-Gastroenterology 52

      Pages: 481-485

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 下咽頭・頸部食道癌手術2005

    • Author(s)
      塩崎 均, 今本治彦, 重岡宏典, 今野元博, 川西賢秀, 彭 英峰
    • Journal Title

      手術 59 (6)

      Pages: 711-718

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 食道癌手術におけ喉頭温存および胃機能温存手術2005

    • Author(s)
      塩崎 均, 今本治彦, 重岡宏典, 今野元博, 平井紀彦, 他
    • Journal Title

      臨床外科 60 (13)

      Pages: 1541-1547

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Oncology emergencyとしての大腸癌イレウスの治療と問題点2005

    • Author(s)
      奥野清隆, 肥田仁一, 石丸英三郎, 上田和毅, 吉藤竹仁
    • Journal Title

      Current Therapy (カレントテラピー) 23 (9)

      Pages: 76-81

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Optimal ligation level of the primary feeding artery and bowel resection margin in colon cancer surgery.2005

    • Author(s)
      J.Hida, K.Okuno, M.Yasutomi, H.Shiozaki, et al.
    • Journal Title

      Dis.Colon Rectum 48(12)

      Pages: 2232-2237

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Number versus distribution in classifying regional lymph node metastases from colon cancer.2005

    • Author(s)
      J.Hida, K.Okuno, M.Yasutomi, H.Shiozaki, et al.
    • Journal Title

      J.Am.Coll.Surg. 201

      Pages: 217-222

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Is preoperative methylprednisolone beneficial for patients undergoing esophagectomy?2005

    • Author(s)
      M.Yano, M.Taniguchi, T.Tsuzinaka, H.Shiozaki, et al.
    • Journal Title

      Hepato-Gastroenterology 52

      Pages: 481-485

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Operation for cervical esophageal cancer.2005

    • Author(s)
      H.Shiozaki, H.Imamoto, H.Shigeoka, M.Imano, K.Kawanishi, H.Ho, et al.
    • Journal Title

      Operation 59(6)

      Pages: 711-718

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Laryngeal function preserving operation for cervical esophageal cancer.2005

    • Author(s)
      H.Shiozaki, H.Imamoto, H.Shigeoka, M.Imano, N.Hirai, K.Kawanishi, et al.
    • Journal Title

      Clinical Surgery 60(13)

      Pages: 1541-1547

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Therapy for colonic ileus by obstructive colorectal cancer as an oncologic emergency.2005

    • Author(s)
      K.Okuno, J.Hida, E.Ishimaru, K.Ueda, T.Yoshifuji
    • Journal Title

      Current Therapy 23(9)

      Pages: 76-81

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunological therapy for liver metastasis2005

    • Author(s)
      K.Okuno
    • Journal Title

      Liver Metastasis ((Ed.) M.Monden, N.Matsuura)

      Pages: 248-255

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 下咽頭・頸部食道癌手術2005

    • Author(s)
      塩崎 均, 今本治彦, 重岡宏典, 今野元博, 川西賢秀, 彭 英峰
    • Journal Title

      手術 59(6)

      Pages: 711-718

    • Related Report
      2005 Annual Research Report
  • [Journal Article] 食道癌手術における喉頭温存および胃機能温存手術2005

    • Author(s)
      塩崎 均, 今本治彦, 重岡宏典, 今野元博, 平井紀彦, 他
    • Journal Title

      臨床外科 60(13)

      Pages: 1541-1547

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Oncology emergencyとしての大腸癌イレウスの治療と問題点2005

    • Author(s)
      奥野清隆, 肥田仁一, 石丸英三郎, 上田和毅, 吉藤竹仁
    • Journal Title

      Current Therapy(カレントテラピー) 23(9)

      Pages: 76-81

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparison of gene-expression profiles between diffuse-and intestinal-typegastric cancers using a genome-wide cDNA microarray2004

    • Author(s)
      Hitoshi Shiozaki
    • Journal Title

      Oncogene Oncogene 33

      Pages: 830-844

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Treatment of Hepatocellular Carcinoma with Percuttaneous Radiofrequency Ablation : Usefulness of Contrast Harmonic Sonography for lesions Poorly Definedwith B-M0de Sonography2004

    • Author(s)
      Hitoshi Shiozaki
    • Journal Title

      American Journal of Roentgnology 183(1)

      Pages: 153-153

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Percutaneous Radiofrequency Ablation Guided by Contrast-Enhanced Harmonic Sonography with Aitificial Pleural Effusion for Hepatocelluar Carcinomain the Hepatic Dome2004

    • Author(s)
      Hitoshi Shiozaki
    • Journal Title

      American Journal of Roentgenology 182(5)

      Pages: 1105-1105

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Surgery for Resectable Esophageal Cancer Cancer in Japan2004

    • Author(s)
      Hitoshi Shiozaki
    • Journal Title

      Ann Thorac Cardiovasc Surg 10(2)

      Pages: 69-70

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Comparison of Long-Term Functional Results of C01onic J-Pouch and Straight Anastomosis After Low Anterrior Resection for Rectal Cancer : A Five-Year Follow-Up2004

    • Author(s)
      Hitoshi Shiozaki
    • Journal Title

      Reprinted from Diseases of the Colon & Rectum 47(10)

      Pages: 1578-1578

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 食道癌2004

    • Author(s)
      塩崎均
    • Journal Title

      日本外科学会雑誌 105(4)

      Pages: 275-280

    • NAID

      110004702534

    • Related Report
      2004 Annual Research Report
  • [Book] 治療、免疫療法(編著 門田守人、松浦成昭) : 肝転移のすべて2005

    • Author(s)
      奥野清隆
    • Total Pages
      379
    • Publisher
      永井書店(大阪)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Book] 治療、免疫療法:肝転移のすべて((編著)門田守人、松浦成昭)2005

    • Author(s)
      奥野清隆
    • Total Pages
      379
    • Publisher
      永井書店(大阪)
    • Related Report
      2005 Annual Research Report
  • [Book] 標準外科学第10版 外科的診断法2004

    • Author(s)
      塩崎 均
    • Related Report
      2004 Annual Research Report
  • [Publications] Shiozaki H: "Erythropoietin regulates tumour growth of human malignancies"Carcinogenesis. 24(6). 1021-1029 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiozaki H: "A Phase II Clinical Trial to Evaluate the Effect of Paclitaxel in Patients with Ascites Caused by Advanced or Recurrent Gastric Carcinoma : a New Concept of Clinical Benefit Response for Non-measurable Type of Gastric Cancer"Jpn J Clin Oncol. 33(5). 238-240 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiozaki H: "Total parenteral nutrition entrains the central and peripheral circadian clocks"NEURO REPORT. 14(11). 1457-1461 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiozaki H: "Transcatheter Arterial Chemoembolization of Hepatocellular Carcinoma : Usefulness of Coded Phase-Inversion Harmonic Sonography"Sonography After Hepatocellular Carcinoma Chemoembolization. 180(4). 703-708 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shiozaki H: "Concurrent Chemoradiotherapy with Protracted Infusion of 5-FU and Cisplatin for Postoperative Recurrent or Residual Esophageal Cancer"Jpn J Clin Oncol. 33(7). 341-345 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okuno K: "Role of sialy Lewis X in liver metastasis in view of liver associated immunity"Hepato-Gastroenterol. 50. 756-760 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 塩崎 均: "食道外科の要点と盲点・頚部食道癌のリンパ節郭清"文光堂. 172-179 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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