| Project/Area Number |
15300151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
HARASHIMA Hideyoshi Hokkaido Univ., Grad.School of Pharm.Sci., Prof., 大学院・薬学研究科, 教授 (00183567)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Hiroyuki Hokkaido Univ., Grad.School of Pharm.Sci., Asso.Prof., 大学院・薬学研究科, 助教授 (10204629)
AKITA Hidetaka Hokkaido Univ., Grad.School of Pharm.Sci., Assist., 大学院・薬学研究科, 助手 (80344472)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥17,300,000 (Direct Cost: ¥17,300,000)
Fiscal Year 2004: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 2003: ¥11,300,000 (Direct Cost: ¥11,300,000)
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| Keywords | gene delivery system / endocytosis / octaarginin / programmed packaging / MEND / アデノウイルス / 非ウイルスベクター / 定量的評価 |
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| Research Abstract |
A rational strategy is required to overcome cellular and intracellular barriers and to develop an innovative non-viral gene delivery system. A new concept "Programd Packaging", for the design and assembling of intelligent nano devices into an integrated, unique non-viral gene delivery system has been developed. Based on this concept, the importance of cellular entrance for a gene delivery system, using Tf as a receptor-mediated endocytic pathway and octaarginine as a non-endocytic pathway was chosen. The advantage of a non-endocytic pathway is demonstrated by an octaarginine-modified envelope type nano device, which exerts transfection activities as high as an adenovirus. Finally, a three dimensional quantitative assay for the intracellular trafficking of a gene delivery systems and its application for the pharmacokinetic optimization of a delivery system is described. These new approaches will ultimately lead to the development of a non-viral gene delivery system which is superior to viral vectors not only from the point of view of safety, but efficiency as well.
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