Search for facto s that induce transdifferentiation from liver stem cell to epitheial cells of digestive gland and development with tissue engineering

• Project/Area Number: 15300154
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Akita University
• Principal Investigator: SUGIYAMA Toshihiro Akita University, School of Medicine, Professor, 医学部, 教授 (00127242)
• Co-Investigator(Kenkyū-buntansha): UENO Yasuharu Akita University, School of Medicine, Assistant Professor, 医学部, 助手 (60375235) ; 亀田 隆 秋田大学, 医学部, 講師 (00301119) ; 寺田 邦彦 秋田大学, 医学部, 助教授 (60197796)
• Project Period (FY): 2003 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥16,600,000 (Direct Cost: ¥16,600,000); Fiscal Year 2005: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000); Fiscal Year 2003: ¥7,600,000 (Direct Cost: ¥7,600,000)
• Keywords: liver stem cell / growth factor / collagen / β-cell / oncostatin M / HSL cell / 細胞移植 / 放線菌

Research Abstract

Organ restitution using somatic stem cells is of great clinical interest. Recent advances in the field of tissue engineering have demonstrated that the use of collagen matrices as scaffolds facilitates tissue reconstruction. Here we have examined the efficacy of transplantation of hepatic stem-like (HSL) cells, a previously established liver epithelial cell line with a potential for differentiation, using collagen scaffolds. To this end, HSL cells were transplanted into Nagase's analbuminemic rat with spongy or gelatinous type I collagen matrices. Consequently, immunohistochemical analyses and genomic PCR experiments revealed engraftment of the transplanted cells. Furthermore, the levels of serum albumin in recipient rats were found to increase up to 2.5 fold relative to controls after transplantation. These findings suggest that HSL cells are able to differentiate into functional hepatocytes in vivo, and that biodegradable collagen matrices may enhance this phenomenon by providing an appropriate microenvironment for hepatocytic repopulation.
To apply cell transplantation for treatment of diabetes mellitus, a sufficient number of beta-cell sources are required. In the present study, we examined whether HSL cells could be a potential beta-cell source. RT-PCR analysis revealed that a series of transcriptional factors involved in differentiation of pancreatic endocrine cells was induced by the treatment with sodium butyrate and betacellulin. HSL cells obtained from adult normal liver also have the potential to differentiate into pancreatic endocrine cells in vitro. HSL cells may be one of the potential beta-cell sources for cell transplant therapy for insulin-dependent diabetes.

Research Products

• [Journal Article] Roles of forkhead transcription factor Foxc2 (MFH-1) and endothelin receptor A in cardiovascular morphogenesis (2005)
  • Author(s): Kanzaki-Kato N. et al.
  • Journal Title: Cardiovasc. Res., 65 Pages: 711-718
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Application of genetically modified feeder cells for culture of keratinocytes. (2005)
  • Author(s): Kameda T. et al.
  • Journal Title: Methods Mol. Biol., 289 Pages: 29-38
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transplantation of rat hepatic stem-like (HSL) cells with collagen matrices. (2005)
  • Author(s): Ueno Y.et al.
  • Journal Title: Hepatol. Res., 33 Pages: 277-284
  • NAID: 10016809917
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Repeated immunization induces the increase in fucose content on antigen-specific IgG N-linked oligosaccharides. (2005)
  • Author(s): Guo N.et al.
  • Journal Title: Clin. Biochem., 38 Pages: 149-153
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes. (2005)
  • Author(s): Okaya A. et al.
  • Journal Title: Am. J. Pathol., 166 Pages: 709-719
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Protection of hepatocytes from apoptosis by a novel substance from actinomycetes culture medium. (2005)
  • Author(s): Liu Y.et al.
  • Journal Title: Biomed. Res., 26 Pages: 9-14
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Orally administrated rare earth element cerium induces metallothionein synthesis and increases glutathione in the mouse liver. (2005)
  • Author(s): Kawagoe M. et al.
  • Journal Title: Life Sci., 77 Pages: 922-937
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Amino acid residues conferring the nucleotide binding properties of N-acetyl-D-gurucosamine 2-epimerase (renin binding protein). (2005)
  • Author(s): Takahashi S. et al.
  • Journal Title: Biomed. Res., 26 Pages: 117-121
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Styrene monomer primarily induces CYP2B1 mRNA in the rat liver. (2005)
  • Author(s): Hirasawa F. et al.
  • Journal Title: Xenobiotica 35 Pages: 1089-1099
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differentiation of adult hepatic stem-like cells into pancreatic endocrine cells. (2005)
  • Author(s): Yamada S. et al.
  • Journal Title: Cell Transplant., 14 Pages: 647-653
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The Wilson disease protein ATP7B resides in the late endosomes with Rab7 and the Niemann-Pick C1 protein. (2005)
  • Author(s): Harada M. et al.
  • Journal Title: Am. J. Pathol., 166 Pages: 499-510
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Roles of forkhead transcription factor Foxc2 (MFH-1) and endothelin receptor A in cardiovascular morphogenesis. (2005)
  • Author(s): Kanzaki-Kato N., Tamakoshi T., Fu Y., Chandra A., Itakura T., Uezato T., Tanaka T., Clouthier D.E., Sugiyama T., Yanagisawa M., Miura N.
  • Journal Title: Cardiovasc.Res. 65 Pages: 711-718
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of genetically modified feeder cells for culture of keratinocytes. (2005)
  • Author(s): Kameda T., Sugiyama T.
  • Journal Title: Methods Mol.Biol. 289 Pages: 29-38
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transplantation of rat hepatic stem-like (HSL) cells with collagen matrices. (2005)
  • Author(s): Ueno Y., Nagai H., Watanabe G., Ishikawa K., Yoshikawa K., Koizumi Y., Kameda T., Sugiyama T.
  • Journal Title: Hepatol.Res. 33 Pages: 277-284
  • NAID: 10016809917
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repeated immunization induces the increase in fucose content on antigen-specific IgG N-linked oligosaccharides. (2005)
  • Author(s): Guo N., Liu Y., Masuda Y., Kawagoe M., Uen, Y., Kameda T., Sugiyama T.
  • Journal Title: Clin.Biochem. 38 Pages: 149-153
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Oncostatin M inhibits proliferation of rat oval cells, OC15-5, inducing differentiation into hepatocytes. (2005)
  • Author(s): Okaya A., Kitanaka J., Kitanaka N., Satake M., Kim Y., Terada K., Sugiyama T., Takemura M., Fujimoto J., Terada N., Miyajima A., Tsujimura T.
  • Journal Title: Am.J.Pathol. 166 Pages: 709-719
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protection of hepatocytes from apoptosis by a novel substance from actinomycetes culture medium. (2005)
  • Author(s): Liu Y., Takahashi S., Ogasawara H., Seo H.G., Kawagoe M., Hirasawa F., Guo N., Ueno Y., Kameda T., Sugiyama T.
  • Journal Title: Biomed.Res. 26 Pages: 9-14
  • NAID: 130004903688
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Orally administrated rare earth element cerium induces metallothionein synthesis and increases glutathione in the mouse liver. (2005)
  • Author(s): Kawagoe M., Hirasawa F., Wang S.-C., Liu Y., Ueno Y., Sugiyama T.
  • Journal Title: Life Sci. 77 Pages: 922-937
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Amino acid residues conferring the nucleotide binding properties of N-acetyl-D-gurucosamine 2-epimerase (renin binding protein). (2005)
  • Author(s): Takahashi S., Ogasawara H., Hiwatashi K., Hata K., Hori K., Koizumi Y., Sugiyama T.
  • Journal Title: Biomed.Res. 26 Pages: 117-121
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Styrene monomer primarily induces CYP2B1 mRNA in the rat liver. (2005)
  • Author(s): Hirasawa F., Kawagoe M., Arany S., Koizumi Y., Ueno Y., Sugiyama T.
  • Journal Title: Xenobiotica 35 Pages: 1089-1099
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differentiation of adult hepatic stem-like cells into pancreatic endocrine cells. (2005)
  • Author(s): Yamada S., Terada K., Ueno Y., Sugiyama T., Seno M., Kojima I.
  • Journal Title: Cell Transplant. 14 Pages: 647-653
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The Wilson disease protein ATP7B resides in the late endosomes with Rab7 and the Niemann-Pick C1 Protein. (2005)
  • Author(s): Harada M., Kawaguchi T., Kumemura H., Terada K., Ninomiya H., Taniguchi E., Hanada S., Baba S., Maeyama M., Koga H., Ueno T., Furuta K., Suganuma T., Sugiyama T., Sata M.
  • Journal Title: Am.J.Pathol. 166 Pages: 499-510
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Orally administrated rare earth element cerium induces metallothionein synthesis and increases glutathione in the mouse liver. (2005)
  • Author(s): Kawagoe M.et al.
  • Journal Title: Life Sci., 77 Pages: 922-937
• [Journal Article] Differentiation of adult hepatic stem-like cells into pancreatic endocrin cells. (2005)
  • Author(s): Yamada S. et al.
  • Journal Title: Cell Transplant., 14 Pages: 647-653
• [Journal Article] Roles of forkhead transcription factor Foxc2 (MFH-1) and endothelin receptor A in cardiovascular morphogenesis. (2005)
  • Author(s): Kanzaki-Kato N.et al.
  • Journal Title: Cardiovasc.Res. 65 Pages: 711-718
• [Journal Article] The Wilson Disease Protein ATP7B Resides in the Late Endosomes with Rab7 and the Niemann-Pick C1 Protein (2005)
  • Author(s): Harada M.et al.
  • Journal Title: Am.J.Pathol. 166 Pages: 499-510
• [Journal Article] A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi. (2004)
  • Author(s): Kameda T. et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 315 Pages: 599-602
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Restoration of copper metabolism and rescue of hepatic abnormalities in LEC rats, an animal model of Wilson's disease, by expression of human ATP7B gene (2004)
  • Author(s): Yan M.Y.et al.
  • Journal Title: Biochim. Biophys. Acta 1690 Pages: 208-219
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Enhanced proliferation of hepatic progenitor cells in rats after portal branch occlusion (2004)
  • Author(s): Ise N.et al.
  • Journal Title: Liver Transpl. 10 Pages: 748-754
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase (ATP7B) in human hepatocellular carcinoma. (2004)
  • Author(s): Sugeno H.
  • Journal Title: Anticancer Res., 24 Pages: 1045-1048
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prognostic value of the Cu-transporting ATPase in ovarian carcinoma patients receiving cisplatin-based chemotherapy (2004)
  • Author(s): Nakayama K. et al.
  • Journal Title: Clin. Cancer Res. 10 Pages: 2804-2811
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Peroxisome proliferator-activated receptor gamma ligands, 15-deoxy-Delta12,14-prostaglandin J2, and ciglitazone, induce growth inhibition and cell cycle arrest in hepatic oval cells. (2004)
  • Author(s): Cheng J. et al.
  • Journal Title: Biochem. Biophy. Res. Commun., 322 Pages: 458-464
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifola frondosa). (2004)
  • Author(s): Hiwatashi K. et al.
  • Journal Title: Biosci. Biotechnol. Biochem., 68 Pages: 1395-1397
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Application of collagen scaffolds for hepatic stem-like cell transplantation. (2004)
  • Author(s): Ueno Y.et al.
  • Journal Title: Akita J. Med., 31 Pages: 113-119
  • NAID: 110001046058
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Potential role of p53 mutation in chemical hepatocarcinogenesis of rats. (2004)
  • Author(s): Deng W.G.et al.
  • Journal Title: World J. Gastroenterol., 10 Pages: 46-52
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi. (2004)
  • Author(s): Kameda T., Ikegami K., Liu Y., Terada K., Sugiyama T.
  • Journal Title: Biochem.Biophys.Res.Commun. 315 Pages: 599-602
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Restoration of copper metabolism and rescue of hepatic abnormalities in LEC rats, an animal model of Wilson's disease, by expression of human ATP7B gene. (2004)
  • Author(s): Yan M.Y., Miyoshi I., Hirabayashi M., Su M., Mototani Y., Okamura T., Terada K., Ueda M., Enomoto K., Sugiyama T., Kasai N.
  • Journal Title: Biochim.Biophys.Acta 1690 Pages: 208-219
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhanced proliferation of hepatic progenitor cells in rats after portal branch occlusion. (2004)
  • Author(s): Ise N., Sato T., Yasui O., Watanabe G., Koyama K., Terada K., Sugiyama T., Yamamoto Y.
  • Journal Title: Liver Transpl. 10 Pages: 748-754
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase (ATP7B) in human hepatocellular carcinoma. (2004)
  • Author(s): Sugeno H., Takebayashi Y., Higashimoto M., Ogura Y., Shibukawa G., Kanzaki A., Terada K., Sugiyama T., Watanabe K., Katoh R., Nitta Y., Fukushima T., Koyama Y., Inoue N., Sekikawa K., Ogawa K., Sato Y., Takenoshita S.
  • Journal Title: Anticancer Res. 24 Pages: 1045-1048
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prognostic value of the Cu-transporting ATPase in ovarian carcinoma patients receiving cisplatin-based chemotherapy. (2004)
  • Author(s): Nakayama K., Kanzaki A., Terada K., Mutoh M., Ogawa K., Sugiyama T., Takenoshita S., Itoh K., Yaegashi N., Miyazaki K., Neamati N., Takebayashi Y.
  • Journal Title: Clin.Cancer Res. 10 Pages: 2804-2811
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Peroxisome proliferator-activated receptor gamma ligands, 15-deoxy-Delta12,14-prostaglandin J2, and ciglitazone, induce growth inhibition and cell cycle arrest in hepatic oval cells. (2004)
  • Author(s): Cheng J., Nakamura H., Imanishi H., Liu W., Morisaki T., Sugiyama T., Hada T.
  • Journal Title: Biochem.Biophy.Res.Commun. 322 Pages: 458-464
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Purification and characterization of a novel prolyl aminopeptidase from Maitake (Grifolafrondosa). (2004)
  • Author(s): Hiwatashi K., Hori K, Takahashi K., Kagaya A., Inoue S., Sugiyama T., Takahashi S.
  • Journal Title: Biosci.Biotechnol.Biochem. 68 Pages: 1395-1397
  • NAID: 10013287045
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of collagen scaffolds for hepatic stem-like cell transplantation. (2004)
  • Author(s): Ueno Y., Terada K., Nagai H., Watanabe G., Ishikawa K., Yoshikawa K., Hirayama Y., Endo E., Kameda T., Liu Y., Sugiyama T.
  • Journal Title: Akita J.Med. 31 Pages: 113-119
  • NAID: 110001046058
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Potential role of p53 mutation in chemical hepatocarcinogenesis of rats. (2004)
  • Author(s): Deng W.G., Fu Y., Li Y.L., Sugiyama T.
  • Journal Title: World J.Gastroenterol. 10 Pages: 46-52
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of genetically modified feeder cells for culture of keratinocytes. (2004)
  • Author(s): Kameda T., Sugiyama T.
  • Journal Title: Epidermal Cells-Methods and Protocols (Turksen, K., ed.) (Human Press, Totowa, New Jersey) Pages: 29-38
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A hypothermic-temperature sensitive gene silencing by the mammalian RNAi. (2004)
  • Author(s): Kameda T.et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 315 Pages: 599-602
• [Journal Article] Restoration of copper metabolism and rescue of hepatic abnormalities in LEC rats, an animal model of Wilson's disease, by expression of human ATP7B gene (2004)
  • Author(s): Yan M.Y.et al.
  • Journal Title: Biochim.Biophys.Acta 1690 Pages: 208-219
• [Journal Article] Prognostic value of the Cu-transporting ATPase in ovarian carcinoma patients receiving cisplatin-based chemotherapy (2004)
  • Author(s): Nakayama K.et al.
  • Journal Title: Clin.Cancer Res. 10 Pages: 2804-2811
• [Journal Article] Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. (2003)
  • Author(s): Nakata A. et al.
  • Journal Title: Biochem. Biophys. Res. Commun., 308 Pages: 834-839
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis : an approach from partial decomposition in vitro and retroviral cell marking in vivo. (2003)
  • Author(s): Kameda T. et al.
  • Journal Title: Exp. Cell Res., 283 Pages: 167-183
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages. (2003)
  • Author(s): Okumura K. et al.
  • Journal Title: Hepatology 38 Pages: 104-113
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Pharmacological mechanism in slip-down behavior of mice. (2003)
  • Author(s): Masuda Y. et al.
  • Journal Title: Tohoku J. Exp. Med., 201 Pages: 23-27
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. (2003)
  • Author(s): Miura K. et al.
  • Journal Title: Biochem. Biophys. Res. Commun., 311 Pages: 415-423
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. (2003)
  • Author(s): Harada M. et al.
  • Journal Title: Int. J. Mol. Med., 11 Pages: 293-298
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase in human esophageal carcinoma. (2003)
  • Author(s): Higashimoto M. et al.
  • Journal Title: Int. J. Mol. Med., 11 Pages: 337-341
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase (ATP7B) as a chemoresistance marker in human oral squamous cell carcinoma treated with cisplatin. (2003)
  • Author(s): Miyashita H. et al.
  • Journal Title: Oral Oncol., 39 Pages: 157-162
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Copper-transporting P-type adenosine triphosphatase (ATP7B) is expressed in human gastric carcinoma. (2003)
  • Author(s): Ohbu M.et al.
  • Journal Title: Cancer Lett., 189 Pages: 33-38
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Mutation and expression of the p53 gene during chemical hepatocarcinogenesis in F344 rats. (2003)
  • Author(s): Fu Y.et al.
  • Journal Title: Biochim. Biophys. Acta. 1628 Pages: 40-49
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Production of monoclonal antibodies recognizing human hair follicle keratinocytes. (2003)
  • Author(s): Hatakeyama S. et al.
  • Journal Title: Hybrid Hybridomics 22 Pages: 127-130
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A glycoside of Nicotina tabacum affects mouse dopaminergic behavior. (2003)
  • Author(s): Masuda Y. et al.
  • Journal Title: Methods Find. Exp. Clin. Pharmacol., 25 Pages: 41-43
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Forkhead transcription factor Foxf2 (LUN)-deficient mice exhibit abnormal development of secondary palate. (2003)
  • Author(s): Wang T.et al.
  • Journal Title: Dev. Biol., 259 Pages: 83-94
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The role of IgG carbohydrates in anaphylaxis : a possible way for antigen-specific anaphylaxis treatment by deglycosylated IgG. (2003)
  • Author(s): Guo N.et al.
  • Journal Title: Biomed. Res., 24 Pages: 291-297
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. (2003)
  • Author(s): Nakata A., Kameda T., Nagai H., Ikegami K., Duan Y., Terada K., Sugiyama T.
  • Journal Title: Biochem.Biophys.Res.Commun. 308 Pages: 834-839
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis : an approach from partial decomposition in vitro and retroviral cell marking in vivo. (2003)
  • Author(s): Kameda T., Nakata A., Mizutani T., Terada K., Iba H., Sugiyama T.
  • Journal Title: Exp. Cell Res. 283 Pages: 167-183
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages. (2003)
  • Author(s): Okumura K., Nakamura K., Hisatomi Y., Nagano K., Tanaka Y., Terada K., Sugiyama T., Umeyama K., Matsumoto K., Yamamoto T., Endo F.
  • Journal Title: Hepatology 38 Pages: 104-113
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Pharmacological mechanism in slip-down behavior of mice. (2003)
  • Author(s): Masuda Y., Suzuki M., Takemura T., Sugawara J., Guo N., Liu Y., Kawarada Y., Shimizu T., Sugiyama T.
  • Journal Title: Tohoku J.Exp.Med. 201 Pages: 23-27
  • NAID: 10011990937
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. (2003)
  • Author(s): Miura K., Nagai H., Ueno Y., Goto T., Mikami K., Nakane K., Yoneyama K., Watanabe D., Terada K., Sugiyama 'T., Imai K., Senoo H., Watanabe S.
  • Journal Title: Biochem.Biophys.Res.Commun. 311 Pages: 415-423
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Wilson disease protein ATP7B is localized in the late endosomes in a polarized human hepatocyte cell line. Int. (2003)
  • Author(s): Harada M., Kumemura H., Sakisaka S., Shishido S., Taniguchi E., Kawaguchi T., Hanada S., Koga H., Kumashiro R., Ueno T., Suganuma T., Furuta K., Namba M., Sugiyama T., Sata M.
  • Journal Title: Int.J.Mol.Med. 11 Pages: 293-298
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase in human esophageal carcinoma. (2003)
  • Author(s): Higashimoto M., Kanzaki A., Shimakawa T., Konno S., Naritaka Y., Nitta Y., Mori S., Shirata S., Yoshida A., Terada K., Sugiyama T., Ogawa K., Takebayashi Y.
  • Journal Title: Int.J.Mol.Med. 11 Pages: 337-341
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of copper-transporting P-type adenosine triphosphatase (ATP7B) as a chemoresistance marker in human oral squamous cell carcinoma treated with cisplatin. (2003)
  • Author(s): Miyashita H., Nitta Y., Mori S., Kanzaki A., Nakayama K., Terada K., Sugiyama T., Kawamura H., Sato A., Morikawa H., Motegi K., Takebayashi Y.
  • Journal Title: Oral Oncol. 39 Pages: 157-162
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Copper-transporting P-type adenosine triphosphatase (ATP7B) is expressed in human gastric carcinoma. (2003)
  • Author(s): Ohbu M., Ogawa K., Konno S., Kanzaki A., Terada K., Sugiyama T., Takebayashi Y.
  • Journal Title: Cancer Lett. 189 Pages: 33-38
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mutation and expression of the p53 gene during chemical hepatocarcinogenesis in F344 rats. (2003)
  • Author(s): Fu Y., Deng W., Kawarada Y., Kawagoe M., Ma Y.-Z., Li X., Guo N., Kameda T., Terada K., Sugiyama T.
  • Journal Title: Biochim.Biophys.Acta 1628 Pages: 40-49
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Production of monoclonal antibodies recognizing human hair follicle keratinocytes. (2003)
  • Author(s): Hatakeyama S., Ma Y.-Z., Miura N., Abe S., Kameda T., Sakamoto K., Sugiyama T.
  • Journal Title: Hybrid Hybridomics 22 Pages: 127-130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A glycoside of Nicotina tabacum affects mouse dopaminergic behavior. (2003)
  • Author(s): Masuda Y., Ohnuma S., Kawagoe M., Sugiyama T.
  • Journal Title: Exp.Clin.Pharmacol. 25 Pages: 41-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Forkhead transcription factor Foxf2 (LUN)-deficient mice exhibit abnormal development of secondary palate. (2003)
  • Author(s): Wang T., Tamakoshi T., Uezato T., Shu F., Kanzaki-Kato N., Fu Y., Koseki H., Yoshida N., Sugiyama T., Miura N.
  • Journal Title: Dev.Biol. 259 Pages: 83-94
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of IgG carbohydrates in anaphylaxis : a possible way for antigen-specific anaphylaxis treatment by deglycosylated IgG. (2003)
  • Author(s): Guo N., Liu Y., Masuda Y., Kawarada Y., Kawagoe M., Yoshikawa K., Kameda T., Sugiyama T.
  • Journal Title: Biomed.Res. 24 Pages: 291-297
  • NAID: 130004705173
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Epidermal Cells-Methods and Protocols (2004)
  • Author(s): Kameda T. et al.
  • Total Pages: 450
  • Publisher: Human Press, Totowa
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 象牙芽前駆細胞を用いた歯組織再生方法 (2006)
  • Inventor(s): 杉山俊博, アラニー・シルビア
  • Industrial Property Rights Holder: 秋田大学
  • Filing Date: 2006-02-09
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 抗血液凝固作用を有する希少元素セリウム及びその誘導体 (2005)
  • Inventor(s): 杉山俊博, 川越政美
  • Industrial Property Rights Holder: 秋田大学
  • Filing Date: 2005-04-20
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 抗血液凝固作用を有する希少元素セリウム及びその誘導体 (2005)
  • Inventor(s): 杉山俊博, 川越政美
  • Industrial Property Rights Holder: 秋田大学
  • Filing Date: 2005-04-20
• [Patent(Industrial Property Rights)] 培養温度による哺乳類細胞でのRNAi効果の制御技術 (2004)
  • Inventor(s): 杉山俊博, 亀田 隆
  • Industrial Property Rights Holder: 杉山俊博
  • Filing Date: 2004-03-29
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] アメロブラスト前駆細胞を用いた歯周組織再生技術 (2004)
  • Inventor(s): 杉山 俊博, 亀田 隆, 中田 憲
  • Industrial Property Rights Holder: 杉山 俊博, (株)エーティーラボ
  • Filing Date: 2004-02-09
• [Patent(Industrial Property Rights)] アメロブラスト前駆細胞を用いた歯周組織再生技術 (2003)
  • Inventor(s): 杉山俊博, 亀田 隆, 中田 憲
  • Industrial Property Rights Holder: 株式会社エーティーラボ, 杉山俊博
  • Filing Date: 2003-09-01
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kameda T, et al.: "A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi"Biochem.Biophys.Res.Commun.. 315. 599-602 (2004)
• [Publications] Miura K, et al.: "Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact"Biochem.Biophys.Res.Commun.. 311. 415-423 (2003)
• [Publications] Nakata A, et al.: "Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line"Biochem.Biophys.Res.Commun.. 308. 834-839 (2003)
• [Publications] Okumura K, et al.: "Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages"Hepatology. 38. 104-113 (2003)
• [Publications] Fu Y, et al.: "Mutation and expression of the p53 gene during chemical hepatocarcinogenesis in F344 rats"Biochim.Biophys.Acta. 1628. 40-49 (2003)
• [Publications] Kameda T, et al.: "Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis"Exp.Cell Res.. 283. 167-183 (2003)