Development of the high-resolution optical tomography capable for in vivo observation of biological tissue structure and the application to early-stage diagnosis of superficial cancer
| Project/Area Number |
15300162
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
HARUNA Masamitsu Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (20029333)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Nariaki Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (70190402)
SEKIMOTO Mitsugu Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (10273658)
OHMI Masato Osaka University, Graduate School of Medicine, Research Associate, 医学系研究科, 助手 (60273645)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥9,000,000 (Direct Cost: ¥9,000,000)
Fiscal Year 2004: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2003: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | High-resolution optical tomography / In-focus OCT / Hi-speed OCT / fibro sarcoma / Early-stage diagnosis of stomach cancer |
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| Research Abstract |
Our research target is proposal and demonstration of a novel high-resolution optical coherence tomography(Hi-OCT) for early-stage diagnosis of stomach cancer. Our proposed technique is used for in-focus imaging method over the entire cross-sectional area of interest. This tomography has the spatial resolution of 3μm with the imaging depth of more than 3mm.
(1)A mode-locked Ti : sapphire laser (femto-second laser) is used as the light source of the Hi-OCT. The coherence length of femto-second laser of 3.1μm was obtained by compensation of unbalance of chromatic dispersion of the low-coherence interferometer.
(2)In order to obtain in-focus imaging over the entire cross-sectional area of interest, we used the focusing effect of the focusing lens of the sample. This method is called in-focus OCT, in which multiple OCT is made by shifting the focal plane of the objective inside of tissue, followed by piling up of the OCT images. The imaging depth of more than 3mm was successfully obtained.
(3)High-speed OCT imaging is required for a real-time OCT with minimization of motion artifact. We proposed the OCT imaging system with the PZM delay line scanning, where two identical PZMs are driven in push-pull operation. The delay-line scanning speed was 100 scans/sec, and an OCT image consists of 400 scans, providing us the image data acquisition time of 4 sec.
(4)In the gastrointestinal application, the Hi-OCT imaging can provide very clear imaging of muscularis mucosae, which is a bending film-like tissue of a few tens micron thickness, showing that our method is useful for the early-stage diagnosis of stomach cancer.
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Report
(3 results)
Research Products
(60 results)
