| Project/Area Number |
15300182
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Medical systems
|
|---|
| Research Institution | KYOTO UNIVERSITY |
|---|
Principal Investigator |
GEN Shokyu Kyoto University, Institute for Frontier Medical Sciences, Associate Professor, 再生医科学研究所, 助教授 (90283655)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TSUTSUMI Sadami Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (00028739)
WADA Hiromi Kyoto University, Graduate School of Medicine and Faculty of Medicine, Professor, 大学院・医学研究科, 教授 (90167205)
NAKAMURA Takashi Kyoto University, Graduate School of Medicine and Faculty of Medicine, Professor, 大学院・医学研究科, 教授 (10201675)
KOMEDA Masasi Kyoto University, Graduate School of Medicine and Faculty of Medicine, Professor, 大学院・医学研究科, 教授 (20303810)
TANAKA Kouichi Kyoto University, Graduate School of Medicine and Faculty of Medicine, Professor, 大学院・医学研究科, 教授 (20115877)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥16,400,000 (Direct Cost: ¥16,400,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2003: ¥9,100,000 (Direct Cost: ¥9,100,000)
|
|---|
| Keywords | EGCG / living tissue preservation / transplantation / cornea / islet / platelet / intimal hyperplasia / immunotolerance / 膵島及び皮膚組織 / 内膜肥厚抑制 / ヒト角膜 / 免疫抑制効果 / 血小板保存 / 神経保存 / エピガロカテキンガレート / ポリフェノール / 細胞増殖制御 / 角膜保存 / 膵島保存 / 生体組織移植 |
|---|
| Research Abstract |
Cornea : Addition of 200ug/mL of EGCG could extend the preservation period of rabbit and human cornea. After 2 weeks preservation, the proliferation ability and morphology of epithelial cells and endothelial cells.
Islet and skin : 30-120ug/mL of EGCG could retain the viability and insulin producing ability of islet after cryopreservation. It was confirmed that skin was preserved better with EGCG than control.
Nerve : It was found that rat sciatic nerves preserved with EGCG were able to allograft and a rejection did not occur and retained the function.
Effect of immunotolerance : It was found that the amount of IL-2 decreased from 100-200ug/mL of EGCG treated lymphocytes less than 1/3. Lymphocyte transplantation revealed the immuno-tolerance ability of EGCG.
Inhibition of intimalhyperplasia : 0.1-1.0mg/mL of EGCG treatment of vein grafts is an effective method to prevent neointimal hyperplasia following coronary artery bypass grafting (CABG).
Platelet : It was found that platelet functions were retained after 1 week preservation with 50-100ug/mL of EGCG.
|
