| Project/Area Number |
15360220
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Measurement engineering
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
ENPUKU Keiji KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Professor, 大学院・システム情報科学研究院, 教授 (20150493)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINAGA Kohji Kyushu Institute of Technology, Department of Applied Chemistry, Professor, 工学部, 教授 (00040436)
KISS Takanobu KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Associate Professor, 大学院・システム情報科学研究院, 助教授 (00221911)
NOZAKI Yukio KYUSHU UNIVERSITY, Graduate School of Information Science and Electrical Engineering, Research Assistant, 大学院・システム情報科学研究院, 助手 (30304760)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,700,000 (Direct Cost: ¥14,700,000)
Fiscal Year 2005: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2003: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | SQUID sensor / High Tc superconductor / Magnetic nanoparticle / Magnetic relaxation / magnetic marker / Immunoassay / antigen-antibody reaction / 吸着 |
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| Research Abstract |
Immunoassays are widely used to detect disease-related proteins for medical diagnostics. These proteins are generically called antigens, and the so-called binding reaction between antigen and its antibody is used for the immunoassay. In order to improve the performance of the immunoassay, we have developed a new detection system utilizing a magnetic marker and a SQUID sensor. The main results obtained in this study are as follows.
(1)We clarified the dependence of the magnetic properties on the size of the magnetic nanoparticle. We showed that the particle with nominal diameter of 25 nm can keep remanence, and can generate large magnetic signal. This particle is, therefore, suitable for magnetic immunoassay.
(2)We developed a coating process to cover the magnetic particle with polymer. With this process, we fabricated a polymer-coated magnetic nanoparticle that shows good dispersion in liquid.
(3)We developed the high Tc SQUID system for detecting magnetic signal from the marker. Since the signal decays rapidly in space, we realized a close distance between the cooled SQUID and the room temperature sample.
(4)We developed a magnetic-contamination free reaction chamber and a method to cancel environmental noise in order to detect very weak signal. Our system can detect 12 samples in a short measurement time of a few minutes with a field resolution of about 10 pT.
(5)We conducted a detection of protein called IgE. We demonstrated the detection of IgE down to 0.3 pg, which shows the high sensitivity of the present magnetic method. We also demonstrated the detection of IgE in human serum, which shows the possibility to practical diagnosis.
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