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Identification of genes for chlorophyll biosynthesis and its roles in stay green phenotype and cell death

Research Project

Project/Area Number 15370015
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

TANAKA Ayumi  HOKKAIDO Univ., Institute of Low Temperature Science, Prof., 低温科学研究所, 教授 (10197402)

Co-Investigator(Kenkyū-buntansha) TANAKA Ryouichi  HOKKAIDO Univ., Institute of Low Temperature Science, Assistant Prof., 低温科学研究所, 助手 (20311516)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 2005: ¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 2004: ¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 2003: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsChlorophyll / Photosynthesis / Metabolism / Chloroplast / 光エネルギー / 発育
Research Abstract

Recently, significant progress has also been made toward elucidating the linkages between chlorophyll metabolism and other cellular processes including leaf senescence, programmed cell death, and plastid signaling. The purpose of the present research is to complete chlorophyll metabolic pathway and to identify all the genes involved in the pathway. Chlorophyll metabolism has been extensively studied with various organisms and almost all of the chlorophyll biosynthetic genes have been identified in higher plants. However, only the gene for 3, 8-divinyl protochlorophyllide a 8-vinyl reductase (DVR), which is indispensable for the photosynthetic organisms to produce monovinylchlorophyll or dinvinyl chlorophyll, has not been identified yet. In this study, we isolated an Arabidopsis mutant that accumulated divinyl chlorophyll instead of monovinyl chlorophyll by ethyl-methane sulfonate mutagenesis. Map-based cloning ofthis mutant resulted in the identification of a gene (At5g18660) that shows sequence similarity with isoflavon reductase genes. The mutant phenotype was complemented by the transformation with the wild-type gene. A recombinant protein encoded by At5g18660 was expressed in E.coli and found to catalyze the conversion of divinyl chlorophyllide to monovinyl chlorophyllide ; thereby indicating that the gene encodes a functional DVR. DVR is encoded by a single-copy gene in the Arabidopsis genome. With the identification of DVR, finally all genes required for chlorophyll biosynthesis have been identified in higher plants. Analysis of the complete genome of Arabidopsis showed that it has 15 enzymes encoded by 27 genes for chlorophyll biosynthesis from 3 glutamyl-tRNAglu to chlorophyll b.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (20 results)

All 2006 2005 2004 Other

All Journal Article (13 results) Patent(Industrial Property Rights) (1 results) Publications (6 results)

  • [Journal Article] Effects of oxidative stress on chlorophyll biosynthesis in cucumber (Cucumis sativus) cotyledons.2006

    • Author(s)
      P.D., Aarti, Tanaka, R., Tanaka, A.
    • Journal Title

      Physiol. Plant. (In press.)

      Pages: 2006-2006

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of oxidative stress on chlorophyll biosynthesis in cucumber (Cucumis sativus) cotyledons.2006

    • Author(s)
      P.D., Aarti, Tanaka, R., Tanaka, A.
    • Journal Title

      Physiol.Plant. (In press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] The N-terminal domain of chlorophyllide a oxygenase confer protein instability in response to chlorophyll b accumulation.2005

    • Author(s)
      Yamasato, A., Nagata, N., Tanaka, R., Tanaka, A.
    • Journal Title

      The Plant Cell. 17(5)

      Pages: 1585-1597

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Ultrafast Excitation Relaxation Dunamics of Lutein in Solution and in the Light-Harvestion Complexes II Isolated from Arabidopsis thaliana.2005

    • Author(s)
      Akimoto, S., Yokono, M., Ohmae, M., Yamazaki, I., Tanaka, A., Higuchi, M., Tsuchiya, T., Miyashita, H., Mimuro, M.
    • Journal Title

      The Journal of Physical Chemistry B 109(25)

      Pages: 12612-12619

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Excitation energy transfer in the antenna system with divinyl-chlorophylls in the vinyl reductase-expressing Arabidopsis.2005

    • Author(s)
      Akimoto, S., Yokono, M., Ohmae, M., Yamazaki, I., Nagata, N., Tanaka, R., Tanaka, A., Mimuro, M.
    • Journal Title

      Chemical Physics Letters 409

      Pages: 167-171

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Effects of Chlorophyllide a Oxygenase Overexpression on Light Acclimation inArabidopsis thaliana.2005

    • Author(s)
      Tanaka, R., Tanaka, A.
    • Journal Title

      Photosynthesis Research 85(3)

      Pages: 327-340

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Effects of Chlorophyllide a Oxygenase Overexpression on Light Acclimation in Arabidopsis thaliana.2005

    • Author(s)
      Tanaka, R., Tanaka, A.
    • Journal Title

      Photosynthesis Research 85(3)

      Pages: 327-340

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Ultrafast Excitation Relaxation Dynamics of Lutein in Solution and in the Light-Harvestion Complexes II Isolated from Arabidopsis thaliana.2005

    • Author(s)
      Akimoto, S., Yokono, M., Ohmae, M., Yamazaki, I., Tanaka, A., Higuchi, M., Tsuchiya, T., Miyashita, H., Mimuro, M.
    • Journal Title

      The Journal of Physical Chemistry B 109(25)

      Pages: 12612-12619

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Identification of a vinyl reductase gene for chlorophyll synthesis in Arabidopsis thaliana and implications for the evolution of Prochlorococcus species.2005

    • Author(s)
      N.Nagata
    • Journal Title

      The Plant Cell 17

      Pages: 233-240

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The N-terminal domain of chlorophyllide a oxygenase confer protein instability in response to chlorophyll b accumulation.2005

    • Author(s)
      A.Yamasato
    • Journal Title

      The Plant Cell In press

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The in vivo and in vitro reconstitution of pigment-protein complexes, and its implication in acquiring a new system.2004

    • Author(s)
      M.Mimuro
    • Journal Title

      Photosyn.Res. 81

      Pages: 129-137

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Synthesis of chlorophyll b : localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit.2004

    • Author(s)
      L.L.Eggink
    • Journal Title

      BMC Plant Biol. 15

      Pages: 5-20

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Unique constitution of photosystem I with a novel subunit in the cyanobacterium Gloeobacter violaceus PCC 7421.2004

    • Author(s)
      H.Inoue
    • Journal Title

      FEBS Letters 578

      Pages: 275-279

    • Related Report
      2004 Annual Research Report
  • [Patent(Industrial Property Rights)] アシフルオロフェンに対する耐性を付与する活性を有するプロトポルフィリノーゲンオキシターゼ及びその遺伝子2005

    • Inventor(s)
      田中 歩
    • Industrial Property Rights Holder
      田中 歩
    • Industrial Property Number
      2005-278942
    • Filing Date
      2005-09-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Publications] Duncan J, Bibby: "Exploring the ability of chlorophyll b to bind to the CP43' protein induced under iron deprivation in a mutant of Synechocystis PCC 6803 containin"FEBS Lett. 541. 171-175 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, R: "The Arabidopsis-accelerated cell death gene ACDI is involved in oxygenation of pheophorbide a : Inhibition of the pheophorbide a oxygenase activity does not lead to the "stay-green" phenotype in Arabidopsis"Plant Cell Physiol.. 44. 1266-1274 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nagata N: "Domain structures of chlorophyllide a oxygenase of green plants and Prochlorothrix hollandica in relation to catalytic functions."Planta. (In press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tanaka, A: "光合成エネルギー捕捉装置の調節"J.Appl.Glycosci. 50. 507-508 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田中 歩: "クロロフィル代謝と植物の発育"化学と生物. 42. 93-98 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 光合成研究会: "光合成事典"学会出版. 410 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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