Analyses of cross-talk between different photoreceptors in plant light signal transduction
| Project/Area Number |
15370020
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理・分子
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAGATANI Akira Kyoto University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40183082)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2004: ¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 2003: ¥7,900,000 (Direct Cost: ¥7,900,000)
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| Keywords | Light response / Signal transduction / Phytochrome / Cryptochrome / Elongation growth / flowering / Blue light response / Arabidopsis / クリプトクロム |
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| Research Abstract |
Morphology of plants depends very much on the light environment. In those responses, which are often referred to as photomorphogenesis, a red/far-red light photoreceptor, phytochrome, and a blue light photoreceptor, cryptochrome play a key roles. In this year, we investigated how the signal from these photoreceptors are integrated to coordinately regulate the plant's morphogenesis. A brief summary of the achievement is as follows.
1)The PHY domain consisting of about 200 amino acid residues is recognized as a sub-domain of phytochrome within the photosensory domain of phytochrome (about 650 amino acid residues). We examined the signaling activity of the N-terminal domain lacking this PHY sub-domain. The results demonstrated that the PHY domain was dispensable for the signaling but was required to keep the active form of phytochrome stable. In addition, we identified many missense mutants within the N-terminal photosensory domain of phyB. Analysis of these mutations suggested different roles for different sub-domains of phytochrome. 2)We examine the involvement of phytochromes and cryptochrimes in the light-induced root greening. Consequently, we found that both the photoreceptors needed to be activated to case the full root greening. We are currently testing whether or not fragments of phyB have similar activity. 3)We screened for mutants in a T-DNA-based activation-tagged pool of Arabidopsis and identified a causal gene for one of such mutants. The results revealed that over-expression of the CYP72C1 gene reduced the level of active brassinosteroids, which led to partial photomorphogenesis in darkness.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Photoreceptors and photoresponses2004
Author(s)
TANAKA S-I, A.NAGATANI
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Journal Title
In "Cross talk between environmental responses and morphogenesis in plants" edited by A.Oka, K.Okada and K.Shinozaki (in Japanese)
Pages: 3-11
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis.2003
Author(s)
Yamamoto, Y., E.Sato, T.Shimizu, N.Nakamichi, S.Sato, T.Kato, S.Tabata, A.Nagatani, T.Yamashino, T.Mizuno
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Journal Title
Plant Cell Physiol. 44
Pages: 1119-1130
NAID
Description
「研究成果報告書概要(和文)」より
Related Report
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[Journal Article] Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis2003
Author(s)
YAMAMOTO, Y., E.SATO, T.SHIMIZU, N.NAKAMICH, S.SATO, T.KATO, S.TABATA, A.NAGATANI, T.YAMASHINO, T.MIZUNO
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Journal Title
Plant Cell Physiol. 44
Pages: 1119-1130
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet implicated in circadian rhythm, control of flowering time, and early photomorphogenesis.2003
Author(s)
Yamamoto, Y., E.Sato, T.Shimizu, N.Nakamich, S.Sato, T.Kato, S.Tabata, S.Nagatani, T.Yamashino, T.Mizuno
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Journal Title
Plant Cell Physiol. 44
Pages: 1119-1130
NAID
Related Report
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[Publications] Tamura, K, T.Shimada, E.Ono, Y.Tanaka, Nagatani, S., Higashi, M., Watanabe, M.: "Why green fluorescent fusion proteins have not been observed in the vacuole of higher plants"Plant J.. 35. 545-555 (2003)
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[Publications] Yamamoto, Y., E.Sato, T.Shimizu, Nakamich, S., Sato, T., Kato, S., Tabata, A.: "Comparative genetic studies on the APRR5 and APRR7 genes belonging to the APRR1/TOC1 quintet in circadian rhythm, control of flowering time, and early photomorphogenesis"Plant Cell Physiol.. 44. 1119-1130 (2003)
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