Importance of copper chaperone Cox17p in early embryogenesis.

• Project/Area Number: 15370050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: University of Tsukuba
• Principal Investigator: KAKO Koichiro University of Tsukuba, Graduate School of Life and Environmental Sciences, Assistant Professor (60311594)
• Co-Investigator(Kenkyū-buntansha): KODAMA Hiroko Teikyo University of School of Medicine, Department of Pediatrics, Associate Professor (00093386)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥6,500,000); Fiscal Year 2004: ¥2,700,000 (Direct Cost: ¥2,700,000); Fiscal Year 2003: ¥3,800,000 (Direct Cost: ¥3,800,000)
• Keywords: Cox17p / mitochondria / cytochrome c oxidase / Sco1 / 2p / central nervous system / in situ hybridization / 細胞内銅輸送機構

Research Abstract

It is well known that the deficiencies in cytochrome c oxidase (CCO) are relatively rare but most often lethal. CCO, one of the mitochodrial respiratory enzymes, requires Cu (I) to express its enzymatic activity. Cox17p, Scolp, and its homologue Sco2p are thought to be involved in mitochondrial transport of copper to CCO. By in vitro GST-pull down assay, both Sco1p and Sco2p interact with Cox17p. Furthermore, Cox17p, Scolp and Sco2p also interact with Cox2p, a subunit of the binuclear-copper centre Cu_A of CCO. Northern blot analysis demonstrated that Cox17p, Sco1p and Sco2p are widely expressed at varying levels in a variety of mouse tissues including brain. Using in situ hybridization histochemistry, we characterized the expression profiles for these molecules in different regions of the normal adult mouse brain. The highest levels of these messages were associated with distinct neuronal subtypes, including pyramidal cell layers of hippocampus and ventral cochlear nucleus of pons, that were also contained high levels of CCO activity. Although CCO activities and expression levels of Cox17p and Scolp were relatively high, the expression of Sco2p was quite low in some subsets of neurons such as cerebellum Purkinje cells. It was thought that the difference of expression profile between Sco1p and Sco2p is originated in that of genomic structures, especially the promoter regions.

Research Products

• [Journal Article] Transcriptional down-regulation through nuclear exclusion of EWS methvlated bv PRMT1. (2005)
  • Author(s): 新谷奈津美, 他
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 653-660
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] 初期胚発生におけるミトコンドリア機能の重要性 (2005)
  • Author(s): 加香孝一郎, 他
  • Journal Title: 化学と生物 43 Pages: 700-702
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transcriptional down-regulation through nuclear exclusion of EWS methylated by PRMT1 (2005)
  • Author(s): Araya, N., Hiraga, H., Kako, K., Arao, Y., Kato, S., Fukamizu, A.
  • Journal Title: Biochem.Biophys.Res.Commun. 329 Pages: 653-660
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Transcriptional down-regulation through nuclear exclusion of EWS methylated by PRMT1. (2005)
  • Author(s): 新谷奈津美他
  • Journal Title: Biochem.Biophys.Res.Commun. 329(2) Pages: 653-660
• [Journal Article] A selective requirement for copper-dependent activation of cytochromec oxidase by Cox17p. (2004)
  • Author(s): 加香孝一郎, 他
  • Journal Title: Biochem.Biophys.Res.Commun. 324 Pages: 1379-1385
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A selective requirement for copper-dependent activation of cytochrome coxidase by Cox17p (2004)
  • Author(s): Kako, K., Takehara, A., Arai, H., Onodera, T., Takahashi, Y., Hanagata, H., Ogra, Y., Takagi, H., Kodama, H., Suzuki, K.T., Munekata, E., Fukamizu, A.
  • Journal Title: Biochem.Biophys.Res.Commun. 324 Pages: 1379-1385
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A selective requirement for copper-dependent activation of cytochrome c oxidase by Cox 17p. (2004)
  • Author(s): 加香孝一郎他
  • Journal Title: Biochem.Biophys.Res.Commun. 324(4) Pages: 1379-1385
• [Journal Article] Mammalian copper chaperone Cox17p is indispensable for cellular resniration and embrvonic develonment. (2003)
  • Author(s): 高橋良徳, 他
  • Journal Title: J.Peptide.Sci. 8
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Highly conserved Drosophila ananassae timeless gene functions as a clock component in Drosophila melanogaster (2003)
  • Author(s): 西ノ首いずみ, 他
  • Journal Title: Gene 307 Pages: 183-190
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Histochemical analysis of copper chaperone proteins-Cox17p, Sco1p and Sco2p-in different regions of the mouse brain. (2003)
  • Author(s): 加香孝一郎, 他
  • Journal Title: Biomed.Res.Trace Elements 14 Pages: 284-286
  • NAID: 10027904279
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cox17p plays a key role for maintenance of cellular copper homeostasis (2003)
  • Author(s): Kako, K., Onodera, T., Arai, H., Takehara, A., Hanagata, H., Takahashi, Y., Takagi, H., Kodama, H., Munekata, E.
  • Journal Title: Peptide science 2002(Yamada, T., Ed.)(Protein Research Foundation, Osaka) Pages: 119-122
  • NAID: 80015974535
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Mammalian copper chaperone Cox17p. is indispensable for cellular respiration and embryonic development (2003)
  • Author(s): Takahashi, Y., Kako, K., Inada, Y., Arai, H., Munekata., E.
  • Journal Title: J.Peptide.Sci. 8
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Highly conserved Drosophila ananassae timeless gene functions as a clock component in Drosophila melanogaster (2003)
  • Author(s): Nishinokubi, I., Shimoda, M., Kako, K., Sakai, T., Fukamizu, A., Ishida, N.
  • Journal Title: Gene 307 Pages: 183-190
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Histochemical analysis of copper chaperone proteins-Cox17p, Sco1p and Sco2p-in different regions of the mouse brain (2003)
  • Author(s): Kako, K., Takahashi, Y., Inada, Y., Hisano, S., Munekata, E., Fukamizu, A.
  • Journal Title: Biomed.Res.Trace Elements 14 Pages: 284-286
  • NAID: 10027904279
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The importance of mitochondrial function in the mammalian early-embryo-genesis (2003)
  • Author(s): Kako, K., Fukamizu, A., Munekata, E.
  • Journal Title: Kagaku to Seibutsu 43 Pages: 700-702
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Peptide science 2002 (2003)
  • Author(s): 加香孝一郎, 他
  • Total Pages: 4
  • Publisher: Protein Research Foundation, Osaka
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 西の首いずみ: "Highly conserved Drosophila ananassae timeless gene functions as a clock component in Drosophila melanogaster"Gene. 307. 183-190 (2003)
• [Publications] 高橋良徳: "Mammalian copper chaperone Cox17p is indispensable for cellular respiration and embryonic development"J.Peptide Sci.. 8. s192-s193 (2003)
• [Publications] 加香孝一郎: "Histochemical analysis of copper chaperone proteins -Cox17p, Sco1p and Sco2p- in different regions of the mouse brain"Biomed.Res.Trace Elements. 14. 284-286 (2003)
• [Publications] 加香孝一郎: "Cox17p plays a key role for maintenance of cellular copper homeostasis""Peptide science 2002"(Yamada, T., Ed.), Protein Research Foundation, Osaka. 119-122 (2003)