| Project/Area Number |
15370054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
KANAZAWA Hiroshi Osaka University, Graduate School of Science, Professor, 理学研究科, 教授 (50116448)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUYAMA Keiichi Osaka University, Graduate School of Science, Professor, 理学研究科, 教授 (80032283)
NAKAMURA Norihiro Osaka University, Graduate School of Science, Assistant Professor, 理学研究科, 助手 (90324748)
MITSUI Keiji Osaka University, Graduate School of Science, Assistant Professor, 理学研究科, 助手 (60379279)
井上 弘樹 大阪大学, 大学院・理学研究科, 助手 (10294448)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 2004: ¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 2003: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | Salinity torelance / Molecular basis of ion transporters / Na+ / H+ antiporters / genetic and biochemical analyses / bioenergetics of transporters / FRET analyses / Cys scanning mutagenesis / gene engineering / Cys走査変異解析 / Na^+恒常性 / H^+交換輸送担体 / 能動輸送 / 膜蛋白質 / 生物学的統一性 / イオン輸送蛋白 / 機能促進因子 / 分子遺伝学的アプローチ |
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| Research Abstract |
Intracellular ionic conditions are maintained within a certain range for every ions including H^+ and Na^+. Even for the higher salinity conditions of outside of the cells, cells e remove alkaline cations like Na^+ and Li+ from the inside of cells for keeping homeostasis. This exclusion of toxic alkaline cations from inside of cells is performed by Na^+/H^+ antiporters which exists in most of the cells from bacterial to human. To understand the adaptation mechanisms of cells to the high salinity conditions, molecular mechanism of Na^+/H^+ antiporters should be clarified. In this study, we analyzed molecular structure of Na^+/H^+ antipotrer NhaA from E.coli and H pylori mainly by mutagenic and biochemical studies. As the results, we have shown that essential transmembrane segments for ion transport involves TM4,5,10 and 11 and further shown that essential or important residues in these Tms (J.Biol Chem.(2003)). We also established a method by using FRET(fluorescence resonance energy tra
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nsfer) to detect protein conformational change during ion transport (Manuscript submitted). We isolated a series of mutants with substitution to Cys for each residues in the TM 4,5,10 and 11. Analyses of the mutants in terms of antiporter activities and accessibility of N-ethyl maleimide revealed presense of a putative channel like structure within the NhaA surrounded by the Tms (J.Biol Chem.(2004)). We have cloned new isoforms of human Na^+/H^+ antiporters named NHE8 and 9 and revealed their function and intracellular distribution within different intracellular vesicles (J.Biol.Chem.(2005)). These endomembrane type antiporter function as K^+/H^+ antiporter and regulate endosomal pH. We also revealed a new function of CHP we had found a binding partner of NHE1 previously. We have constructed a knockout cells of gene for CHP and found that NHE does not translocate to the cytoplasmic membrane for the knockout cells, suggesting that CHP is required for intracellular destination of NHE to the cytoplasmic membrane which differs from the previous observation by Pang et al. Less
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