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Genetic factors responsible for the mobility of mPing in the rice genome

Research Project

Project/Area Number 15380006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionKyoto University

Principal Investigator

OKUMOTO Yutaka  Kyoto University, Graduate school of Agriculture, Associate Professor, 農学研究科, 助教授 (90152438)

Co-Investigator(Kenkyū-buntansha) TANISAKA Takatoshi  Kyoto University, Graduate school of Agriculture, Professor, 農学研究科, 教授 (80026591)
NAKAZAKI Tetsuya  Kyoto University, Graduate school of Agriculture, Lecturer, 農学研究科, 講師 (60217693)
TERAISHI Masayoshi  Kyoto University, Graduate school of Agriculture, Assistant Professor, 農学研究科, 助手 (80378819)
山田 利昭  京都大学, 農学研究科, 教授 (80959700)
堀端 章  近畿大学, 生物理工学部, 講師 (70258060)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2005: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2004: ¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2003: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsRice / Transposon / MITEs / mechanism of transposition / Mutation / Rurm1 / mPing / MITE / 転移
Research Abstract

In a mutant line IM294 (slender glume mutant) which harbors Rurm1 (Rice Ubiquitine Related Modifier-1) allele inactivated by mPing insertion shows high excision frequency (ca.1%) of mPing from mutant Rurm1 allele (Rurm1^m). Expression levels of two ORFs of Ping were also high in IM294 comparing to the original variety Gimbozu. To clarify the relation between the function of Rurm1 and Ping activity under the same genetic background, the mutant allele of Rurm1 was introduced into Gimbozu background through recurrent backcrossing. Also, a pair of non-slender glume line (Rurm1^+/Rrum1^+) and slender glume line (Rurm1^m/Rurm1^m) derived from offspring of the non-slender glume plant (Rurm1^+/Rurm1^m) segregated among IM294 line were used. Comparing the Gimbozu and its isogenic line and sib lines, inactivation of Rurm1 with mPing insertion enhanced the expression levels of two ORFs of Ping. On the other hand, significant change in the expression level of Pong was not observed. These indicate that the inactivation of Rurm1 promotes the mPing excision by enhancing the expression of Ping's ORFs. Though, the inactivation of transposon is generally regulated by methylation, it is quite unlikely that RURM1 protein directly regulate the methylation of DNA. Effect of the inactivation of Rurm1 allele was also analyzed using 22k-micro array (Agilent Co.,). Inactivation of Rurm1 upregulate the expression of protein biosynthesis related genes in ribosome and nucleus. It also downregulated the expression of genes related to metabolism and physiological process. These indicate that the function of Rurm1 is related to transcription of vast number of genes related to the physiological process. Upregulation of protein synthesis related genes indicates the compensation to growth retardation of plants. Upregulation of genes related to DNA-repair process was also observed indicating the frequent DNA double strands break induced by mPing excision in IM294.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (14 results)

All 2006 2005 2004 Other

All Journal Article (10 results) Publications (4 results)

  • [Journal Article] イネ新規ユビキチン様タンパク質RURM1のクローニング、大腸菌発現および精製2006

    • Author(s)
      築山拓司, 李鐘源, 井上國世, 奥本裕, 中崎鉄也, 谷坂隆俊
    • Journal Title

      育種学研究 8(別1)

      Pages: 37-37

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic factors responsible for the mobility of mPing in the rice genome2006

    • Author(s)
      A.Onishi, T.Tsukiyama, Y.Okamoto, S.Yamahira, M.Morita, T.Nakazaki, T.Tanisaki
    • Journal Title

      Breeding Research vol.7(supple1,2)

      Pages: 134-134

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Cloning, bacterial expression and purification of rice (O.sativa L.) novel ubiquitin-related protein RURM12006

    • Author(s)
      T.Tsukiyama, J.Lee, K.Inoue, Y.Okumoto, T.Nakazaki T.Tanisaki
    • Journal Title

      Breeding Research vol.8(supple.1)

      Pages: 37-37

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] イネ新規ユビキチン様タンパク質RURM1のクローニング、大腸菌発現および精製2006

    • Author(s)
      築山拓司, 李鐘源, 井上國世, 奥本裕, 中崎鉄也, 谷坂隆俊
    • Journal Title

      育種学研究 8(別1)(掲載予定)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イネ非自律性トランスポゾンmPingの転移を促す遺伝要因2005

    • Author(s)
      大西麻紀子, 築山拓司, 奥本裕, 山平諭, 森田美香, 中崎鉄也, 谷坂隆俊
    • Journal Title

      育種学研究 7(別1・2)

      Pages: 134-134

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Function of Rurm1 alleles on the activity of the rice transposon miniature-Ping(mPing)2004

    • Author(s)
      Okumoto, Y., A.Onisi, T.Tsukiyama, T.Nakazaki, T.Tanisaka
    • Journal Title

      2nd International Symposium of Rice Functional Genomics.(poster presentation) 2

      Pages: 140-140

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Rapid amplification of transposon Ping and mPing in Japonica rice varieties2004

    • Author(s)
      Okumoto, Y., K.Naito, T.Nakazaki, A.Horibata, M.Morira, T.Tanisaka
    • Journal Title

      World rice research conference 2004(poster presentation 669) 1

      Pages: 149-149

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Destruction of Rurm1 influences the mobility of mPing in rice(Oryza sativa L.)2004

    • Author(s)
      Onishi, A., Y.Okumoto, S.Yamahira, T.Tsukiyama, T.Nakazaki, T.Tanisaka
    • Journal Title

      World rice research conference 2004(poster presentation 682) 1

      Pages: 149-149

    • Related Report
      2004 Annual Research Report
  • [Journal Article] イネ品種銀坊主におけるトランスポゾンPingおよびmPingの特異的増幅2004

    • Author(s)
      奥本裕, 内藤健, 中崎鉄也, 堀端章, 谷坂隆俊
    • Journal Title

      育種学研究 6(別1)

      Pages: 244-244

    • Related Report
      2004 Annual Research Report
  • [Journal Article] ガンマ線照射はイネトランスポゾンPingの転移を誘導する2004

    • Author(s)
      森田美佳, 内藤健, 奥本裕, 中崎鉄也, 谷坂隆俊
    • Journal Title

      育種学研究 6(別2)

      Pages: 64-64

    • Related Report
      2004 Annual Research Report
  • [Publications] 山平諭, 中崎鉄也, 奥本裕, 堀端章, 谷坂隆俊: "易変性細粒突然変異系統IM294におけるmPingの切出しに関する挿入サイトの効果"育種学研究. 5・別1. 25 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 堀端章, 中崎鉄也, 奥本裕, 谷坂隆俊: "異なる遺伝子座に挿入されているトランスポゾンmPingの培養細胞における転移"育種学研究. 5・別1. 26 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松井和幸, 堀端章, 奥本裕, 中崎鉄也, 谷坂隆俊: "トランスポゾン・ディスプレイ法によるmPing転移のモニタリング"育種学研究. 5・別2. 76 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 奥本裕, 内藤健, 中崎鉄也, 堀端章, 谷坂隆俊: "イネ品種銀坊主におけるトランスポゾンPingおよびmPingの特異的増幅"育種学研究. 6・別1(発表予定). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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