Molecular analysis of plant Na and K transporter involved in response to environmental stress
| Project/Area Number |
15380070
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
UOZUMI Nobuyuki Nagoya University, Bioscience and Biotechnology Center, Professor, 生物機能開発利用研究センター, 教授 (40223515)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 2004: ¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 2003: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | Na transporter / K transporter / K channel / AtHKT1 / HKT / Ktr / Arabidopsis / cyanobacteria / EF-hand / K^+ / らん藻 / KUP / スフェロプラスト / 大腸菌 / プロトン駆動力 |
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| Research Abstract |
1 The Ktr-system in Synechocystis sp.PCC 6803 was found to be Na-activated K transporter. The K transport activity was inhibited by CCCP and was not found in the mutant strain which has a mutation of the respiratory chain system. These indicate that proton motive force is involved in the Ktr-mediated K transport. Hyperosmotic stress led initially to loss of net K from the cells. The ktrB mutant shocked with sorbitol failed to reaccumulate K up to its original level. The K uptake via KtrABE palys a crucial role in the early phase of cell turgor regulation after hyperosmotic shock.
2 Bacterial Ktr system and plant HKT-system has eight transmembrane topology. The positive residue in the eighth transmembrane segment was replaced with other amino acids. The conversion from Arg to Lys sustained the K uptake activity but that to negative or non-polar amino acids gave loss of the function. The positive residue is important for the activity.
3 KUP-type transporter was not inserted into the rough microsomal membrane. Then the transporter was expressed in E.coli and their membrane structure was determined.
4 Some of plant K channels has the putative Ca binding sites. The mutation in the region inhibited the K uptake activity. The region is important for the transport activity.
5 Arabidopsis AtHKT1 transporter was expressed in the vascular tissuels in the aerial part of the plant. The null mutant showed that Na accumulated in the xylem tissue and Na was not supplied into the phloem tissue. AtHKT1 is one of the components for Na recirculation of plant.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Mechanosensitivity of GIRK channels is mediated by protein kinase C-dependent channel-phosphatidylinositol 4,5-bisphosphate interaction.2004
Author(s)
Zhang, L., Lee, J-K., John, S.A., Uozumi, N., Kadama, I.
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Journal Title
J.Biol.Chem. 279
Pages: 7037-7047
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[Journal Article] Na^+-dependent K^+ uptake Ktr system from the cyanobacterium Synechocystis sp. PCC 6803 and its role in the early phases of cell adaptation to hyperosmotic shock.2004
Author(s)
Mtsuda, N., Kobayashi, H., Katoh, H., Ogawa, T., Futatsugi, L., Nakamura, T., Bakker, E.P., Uozumi, N.
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Journal Title
J.Biol.Chem. 279
Pages: 54952-54962
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[Journal Article] Functional analysis of AtHKT1 in Arabidopsis shows that Na^+ recirculation by the phloem is crucial for salt tolerance.2003
Author(s)
Berthomieu, P., Conejero G, Nublat A., Brackenbury W.L.Lambert C., Savio, C., Uozumi, N., Oiki, S., Yamada, K., Cellier, F., Gosti, F., Simonneau, T., Essah, P.A., Tester, M., Very, A-A, Sentenac, H., Casse, F.
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Journal Title
EMBO J. 22
Pages: 2004-2014
Description
「研究成果報告書概要(欧文)」より
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