Investigation of signal transduction pathways of jasmonic acid in higher plants
| Project/Area Number |
15380080
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YAMANE Hisakazu The University of Tokyo, Biotechnology Research Center, Professor (80090520)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,800,000 (Direct Cost: ¥14,800,000)
Fiscal Year 2005: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥7,800,000 (Direct Cost: ¥7,800,000)
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| Keywords | jasmonic acid / chitin olieosaccharide elicitor / 12-oxophytodiehoic acid reductase / bHLH transcription factor / WRKY-type transcription factor / defense responses / disease resistance / Orvza aativa L. / エリシター / ジテルペン環化酵素遺伝子 / ファイトアレキシン / 転写因子 / ジテルペン環化酵素 / フィトアレキシン / オリザレキシンS |
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| Research Abstract |
In this project, in order to have basic information on jasmonic acid (JA) functions and its signal transduction pathways, we have performed extensive studies including promoter analysis of a jasmonic acid (JA)-responsive gene, functional identification of a JA biosynthetic enzyme gene, and detailed characterization of JA/elicitor-responsive transcription factors that play key roles in the regulation of defense responses in rice. The principal conclusions are as follows: (l) We identified a TGACG-containing sequence that is a cis element involved in the JA-responsiveness of OsOPR1 in suspension-cultured rice cells, suggesting that a basic leucine zipper (bZIP) transcription factor is involved in the JA-responsive expression of OsOPR1 transcription. (2) We provided evidence that an OPR family gene in rice chromosome 8, OsOPR7, encodes the enzyme involved in the JA biosynthesis. The expression of OsOPR7mRNA was induced and reached maximum levels within 0.5 h of mechanical wounding and dro
… More
ught stress, and the endogenous JA level started to increase in accordance with the increase in OsOPR7expression. (3) We indicated that the JA-responsive bHLH transcription factor gene RERJ1 isolated from suspension-cultured rice cells was expressed in response to exogenous JA in leaves, leaf sheaths, and roots of rice in a vegetative stage, and that RERJ1 expression in the leaves was up-regulated by exposure to wounding and drought stress. In our recent studies including microarray analysis using transgenic rice overexpressing sense-RERJ1 mRNA, it has been shown that RERJ1 is involved in regulation of pathogenesis-related protein genes expression. (4) We provided strong evidence for the involvement of OsWRKY53 and OsWRKY71 in regulating the expression of defense-related genes induced by chitin oligosaccharide and fungal cerebroside elicitors in rice. Although OsWRKY53 was suggested to be a transcriptional activator, OsWRKY71 showed features characteristic of a transcriptional repressor, indicating that the activation of defense-related genes by OsWRKY71 was probably indirect. Less
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Report
(4 results)
Research Products
(54 results)
