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Genetic Identification of Thermal Resistance and the Development of Distinction Method for Thermal Resistant Individual

Research Project

Project/Area Number 15380129
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionTohoku University

Principal Investigator

NAKAJIMA Masamichi  Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院・農学研究科, 助教授 (20192221)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Nobuhiko  Tohoku University, Graduate School of Agricultural Science, Professor, 大学院・農学研究科, 教授 (20036742)
BARINOVA Anna  東北大学, 大学院・農学研究科・日本学術振興会, 外国人特別研究員
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥16,200,000 (Direct Cost: ¥16,200,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2004: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥11,600,000 (Direct Cost: ¥11,600,000)
KeywordsMicrosatellite / AFLP / Guppy / Thermal resistance / Linkage Analysis / Suppression Subtractive Hybridization / RT-PCR / Caudal fin cell / ヒラメ / ギンブナ / アユ / RT-PCR / サブトランクション法 / サプレッション-サブトラクション法 / 塩基配列 / 相同性 / 遺伝子発現 / 高温処理 / 実験魚 / リンケージマップ / 連鎖解析 / 量的形質 / 交配実験
Research Abstract

To Identify the genetic control of individual differences in thermal resistnace, following experiments were examined. The guppy was used as the experimental model fish for aquaculture, and Ayu, Japanese flounder and silver crucian carp were used as aquaculture fish.
1) The microsatellite DNA markers were developed in the guppy. Total of 49 microsatellite DNA markers were developed.
2) Construct linkage map in the guppy, which is the experimental fish for aquaculture. Total of 33 linkage groups were constructed by 49 microsatellite and AFLP DNA markers.
3) The results of cross experiments indicate that there are major genes affecting thermal resistance on X chromosome.
4) Forty-four fragments of gene which indicated specific activity on high water temperature condition were detected by suppression subtractive hybridization. Six fragments indicated significant similarity with the genes registrated at DDBJ, on the other hand, 44 fragments did not indicate.
5) The results of RT-PCR on the genes which activated on high temperature condition did not indicate significant correlation with the resistant level on individuals and activation level at each genes.
6) The evaluation methods used fin cells was developed on Ayu, Japanese flounder and silver crucian carp.
7) The linkage was observed between the genes which controlling the existence of the black and florescent chromatophore in the guppy.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (23 results)

All 2006 2005 2004 2003 Other

All Journal Article (21 results) Publications (2 results)

  • [Journal Article] グッピーにおける色素胞の有無と連鎖するマイクロサテライトDNAマーカーの探索2006

    • Author(s)
      田口玲平, 中嶋正道, 谷口順彦
    • Journal Title

      水産育種 35

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Search for the microsatellite DNA markers linked with the presence of the chromatophore in the guppy Poecilia reticulata2006

    • Author(s)
      Taguchi, R., M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet.Breed.Sci. 33

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] グッピーにおける色素胞の有無と連鎖するマイクロサテライトDNAマーカーの探索2006

    • Author(s)
      田口玲平, 中嶋正道, 谷口順彦
    • Journal Title

      水産育種 35(印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Linkage mapping of AFLP and microsatellite DNA markers with the body color- and sex-determining loci in the guppy (Poecilia reticulata)2005

    • Author(s)
      Watanabe, T., M.Yoshida, M.Nakajima, N.Taniguchi
    • Journal Title

      Zool. Sci 22

      Pages: 883-889

    • NAID

      110006281866

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal tolerance traits of juvenile of the ayu Plecoglossus altivelis and Japanese flounder Paralichthys olivaceus evaluated by their caudal fin cell2005

    • Author(s)
      Sakamoto, K., M.Nakajima, N.Taniguchi
    • Journal Title

      Aquaculture 246

      Pages: 93-99

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Linkage mapping of AFLP and microsatellite DNA markers with the body color- and sex-determining loci in the guppy (Poecilia reticulata).2005

    • Author(s)
      Watanabe, T., M.Yoshida, M.Nakajima, N.Taniguchi
    • Journal Title

      Zool.Sci. 22

      Pages: 883-889

    • NAID

      110006281866

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal tolerance traits of juvenile of the ayu Plecoglossus altivelis and Japanese flounder Paralichthys olivaccus evaluated by their caudal fin cell.2005

    • Author(s)
      Sakamoto, K., M.Nakajima, N.Taniguchi
    • Journal Title

      Aquaculture 246

      Pages: 93-99

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Linkage mapping of AFLP and microsatellite DNA markers with the body color- and sex-determining loci in the guppy (Poecilia reticulata).2005

    • Author(s)
      Watanabe, T., M.Yoshida, M.Nakajima, N.Taniguchi
    • Journal Title

      Zoological Science 22

      Pages: 883-889

    • NAID

      110006281866

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Construction of six linkage groups in the guppy (Poecilia reticulata).2004

    • Author(s)
      Watanabe, T., M.Nakajima, M.Yoshida, N.Taniguchi
    • Journal Title

      Animal Genet 35

      Pages: 147-147

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] 尾鰭遊離細胞の生残率により評価したクローンギンブナの高温耐性形質の馴致温度による変化2004

    • Author(s)
      有澤佳絋, 中嶋正道, 谷口順彦
    • Journal Title

      水産育種 33

      Pages: 123-127

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal resistance traits in the clonal lines of silver crucian carp Carassius langsdorfii evaluated by caudal fin cells.2004

    • Author(s)
      Sakamoto, K., M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet. Breed. Sci. 33

      Pages: 87-92

    • NAID

      40007147213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Construction of six linkage groups in the guppy (Poecilia reticulata)2004

    • Author(s)
      Watanabe, T., M.Nakajima, M.Yoshida, N.Taniguchi
    • Journal Title

      Animal Genet. 35

      Pages: 147-147

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Thermal resistance traits in the clonal lines of silver crucian carp Carassius langsdorfii evaluated by caudal fin cells.2004

    • Author(s)
      Sakamoto, K., W.Koedprang, M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet.Breed.Sci. 33

      Pages: 87-92

    • NAID

      40007147213

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Change in the thermal tolerance of silver crucian carp at different acclimated temperatures evaluated by the survival of cells isolated from tail fin2004

    • Author(s)
      Arisawa, Y., M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet.Breed.Sci. 33

      Pages: 123-127

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Supression subtraction hybridization analysis on the high temperature resistance in the guppy Poecilia reticulata.2004

    • Author(s)
      Nakajima et al.
    • Journal Title

      29^<th> International Conference on Animal Genetics, ISAG 2004/Tokyo

      Pages: 45-45

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isolation and characterization of 43 microsatellite DNA Markers for guppy (Poecilia reticulata)2003

    • Author(s)
      Watanabe, T., M.Yoshida, M.Nakajima, N.Taniguchi
    • Journal Title

      Mol. Ecol. Note 3

      Pages: 487-490

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ギンブナの低水温耐性形質におけるクローン間差2003

    • Author(s)
      阪本憲司, 中嶋正道, 谷口順彦
    • Journal Title

      水産育種 33

      Pages: 49-54

    • NAID

      40007147210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] ギンブナの海水耐性形質におけるクローン間差2003

    • Author(s)
      阪本憲司, 中嶋正道, 谷口順彦
    • Journal Title

      水産育種 33

      Pages: 55-60

    • NAID

      40007147211

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isolation and characterization of 43 microsatellite DNA Markers for guppy (Poecilia reticulata)2003

    • Author(s)
      Watanabe, T., M.Yoshida, M.Nakajima, N.Taniguchi
    • Journal Title

      Mol.Ecol.Note 3

      Pages: 487-490

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differences of low temperature tolerance among clonal lines in silver crucian carp (Carassius langsdorfii).2003

    • Author(s)
      Sakamoto, K., M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet.Breed.Sci. 33

      Pages: 49-54

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differences of sea water tolerance among clonal lines in silver crucian carp (Carassius langsdorfii).2003

    • Author(s)
      Sakamoto, K., M.Nakajima, N.Taniguchi
    • Journal Title

      Fish Genet.Breed.Sci. 33

      Pages: 55-60

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Watanabe et al.: "Isolation and characterization of 43 microsatellite DNA markers for guppy(Poecilia reticulata)"Molecular Ecology Note. 3(4). 487-490 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Watanabe et al.: "Construction of six linkage groups in the guppy(Poecilia reticulata)"Animal Genetics. (In press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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