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Molecular diet analysis of larvae of important marine animals

Research Project

Project/Area Number 15380137
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionIncorporated Administrative Agency Fisheries Research Agency

Principal Investigator

ICHII Taro  National Research Institute of Far Seas Fisheries, Oceanic Resources Division, Section Chief, 遠洋水産研究所・外洋資源部, 研究室長 (50360871)

Co-Investigator(Kenkyū-buntansha) CHOW Seinen  National Research Institute of Fisheries Science, Coastal Fisheries and Aquaculture Division, Section Chief, 中欧水産研究所・浅海増殖部, 研究室長 (70360766)
MOCHIOKA Noritaka  Kyushu University, Faculty of Agriculture, Assistant Professor, 大学院・農学研究院, 助手 (40212261)
SAKAI Mitsuo  National Research Institute of Far Seas Fisheries, Oceanic Resources Division, Senior Researcher, 遠洋水産研究所・外洋資源部, 主任研究員 (70371937)
YOSHIMURA Taku  Seikai National Fisheries Research Institute, Coastal Fisheries and Aquachulture Division, Section Chief, 西海区水産研究所・海区水産業研究部, 研究室長 (90371997)
YAMADA Harumi  National Research Institute of Far Seas Fisheries, Western Pacific Tuna and Skipjack Resources Division, Section Chief, 遠洋水産研究所・温帯性まぐろ資源部, 研究室長 (10371915)
本多 仁  独立行政法人 水産総合研究センター, 本部, 研究開発官 (90165613)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥8,100,000 (Direct Cost: ¥8,100,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2004: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsspiny lobster / eel / squid / tuna / larvae / stomach content / genome typing
Research Abstract

Since species identification of the host larvae is important to investigate the stomach content, we have developed molecular markers to determine species of spiny lobsters, cephalopods, lantern fishes and eels. Especially, identification of squid larvae significantly contributed to stock assessment and dynamics of squid and fishes utilizing squids. Phylogenetic analysis of tuna species was performed using novel nuclear DNA. Larvae of neon squid, eel, conger eel, scyllarid lobsters and palinurid lobsters were used for molecular diet analysis. In all larvae, fungi and host variant 18S rDNA were detected, and no candidate organism as prey was found in the squid and eels. On the other hand, 18S rDNA of cnidaria and urochordata were found in two (Panulirus longipes bispinosus and P.echinatus) of three palinurid species and one (Scyllarides sp.) of two scyllarid lobster species. Fish DNA was found in the Japanese spiny lobster (P.japonicus).

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (38 results)

All 2006 2005 2004 2003 Other

All Journal Article (36 results) Publications (2 results)

  • [Journal Article] Identification of mid to final stage lobster phyllosoma larvae of the genus Panulirus White, 1847 collected in the Ryukyu2006

    • Author(s)
      Chow, S.
    • Journal Title

      Crustaceana (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular species identification of spiny lobster phyllosoma larvae of the genus Panulirus from the northwestern Pacific.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Marine Biotechnology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A molecular attempt to identify prey organisms of lobster phyllosoma larvae.2006

    • Author(s)
      Suzuki, N.
    • Journal Title

      Fisheries Science 72

      Pages: 314-348

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Identification of ommastrephid squid paralarvae collected in the northern Hawaiian waters and phylogenetic implication in the family Ommastrephidae using mtDNA analysis.2006

    • Author(s)
      Wakabayashi, T.
    • Journal Title

      Fisheries Science (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Phylogenetic relationships among Thunnus tuna species inferred from rDNA ITS1 sequence.2006

    • Author(s)
      Chow, S.-
    • Journal Title

      Journal of Fish Biology 68

      Pages: 24-35

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic divergence and identification between two controversial lanternfishes (Pisces : Myctophidae : Diaphus) based on the mitochondrial cytochrome b sequences and PCR-RFLP analysis.2006

    • Author(s)
      Suzuki, N.
    • Journal Title

      Species Diversity 10

      Pages: 289-299

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Stock assessment of the autumn cohort of neon flying squid (Ommastrephes bartramii) in the North Pacific based on past large-scale high seas driftnet fishery data.2006

    • Author(s)
      Ichii, T.
    • Journal Title

      Fisheries Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Eukaryotes from the hepatopancreas of palinurid lobster phyllosoma larvae.2006

    • Author(s)
      Suzuki, N.
    • Journal Title

      Proceedings of the International Workshop on Spiny Lpbster Seed production Technology (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] イセエビの幼生は自然界で何を食べているのか-分子生物学的アプローチ.2006

    • Author(s)
      張 成年
    • Journal Title

      DNA多型学会シンポジウムプロシーディング (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] DNA分析でイセエビ浮遊幼生の天然餌料を明らかにする試み.2006

    • Author(s)
      張 成年
    • Journal Title

      養殖 2006.4

      Pages: 84-87

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Morphological description of a later stage phyllosoma larva of Panulirus echinatus Smith, 1869 identified by DNA analysis.2006

    • Author(s)
      Konishi, K.
    • Journal Title

      Journal of Plankton Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of mid to final stage lobster phyllosoma larvae of the genus Panulirus White, 1847 collected in the Ryukyu Archipelago.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Crustaceana (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Molecular species identification of spiny lobster phyllosoma larvae of the genus Panulirus from the northwestern Pacific.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Marine Biotechnology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of ommastrephid squid paralarvae collected in the northern Hawaiian waters and phylogenetic implication in the family Ommastrephidae using mtDNA analysis.2006

    • Author(s)
      Wakabayashi, T.
    • Journal Title

      Fisheries Science (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Phylogenetic inferred from among Thunnus tuna species inferred from rDNA ITS1 sequence.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Journal of Fish Biology 68

      Pages: 24-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Stock assessment of the autumn cohort of neon flying squid (Ommastrephes bartramii) in the North Pacific on past large-scale high seas driftner fishery data.2006

    • Author(s)
      Ichii, T.
    • Journal Title

      Fishenies Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Eukaryotes from the hepatopancreas of palinurid lobster phyllosoma larvae.2006

    • Author(s)
      Suzuki, N.
    • Journal Title

      Proceedings of the International Workshop on Spiny Lobster Seed production Technology (in ress)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Morphological description of a later stage phyllosoma larvae of Panulirus echinatus Smith, 1869 identified by DNA analysis.2006

    • Author(s)
      Konishi, K.
    • Journal Title

      Journal of Plankton Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of mid to final stage lobster phyllosoma larvae of the genus Panulirus White, 1847 collected in the Ryukyu Archipelago.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Crustaceana (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Phylogenetic relationships among Thunnus tuna species inferred from rDNA ITS1 sequence.2006

    • Author(s)
      Chow, S.
    • Journal Title

      Journal of Fish Biology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Eukaryotes from the hepatopancreas of palinurid lobster phyllosoma larvae.2006

    • Author(s)
      Suzuki, N.
    • Journal Title

      Proceedings of the International Workshop on Spiny Lobster Seed production Technology (印刷中)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nuclear and mitochondrial DNA markers indicate unidirectional gene flow of Indo-Pacific to Atlantic bigeye tuna (Thunnus obesus) populations, and their admixture off southern Africa.2005

    • Author(s)
      Durand, J.-D.
    • Journal Title

      Marine Biology 142

      Pages: 313-322

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The red flying squid (Ommastrephes bartramii) : a review of recent research and the fishery in Japan.2005

    • Author(s)
      Bower. J.R.
    • Journal Title

      Fisheries Research 76

      Pages: 39-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic isolation between Atlantic and Mediterranean albacore (Thunnus alalunga) populations inferred from mitochondrial and nuclear DNA markers.2005

    • Author(s)
      Nakadate, M.
    • Journal Title

      Journal of Fish Biology 66

      Pages: 1545-1557

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] The red flying squid (Ommastrephes bartramii) : a review of recent research and the fishery in Japan.2005

    • Author(s)
      Bower, J.R.
    • Journal Title

      Fisheries Research 76

      Pages: 39-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Genetic isolation between Atlantic and Mediterranean albacore (Thunnus alalunga) populations inferred from mitochondrial and nuclear DNA markers.2005

    • Author(s)
      Nakadate, M.
    • Journal Title

      Journal of Fish Biology (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Nuclear and mitochondrial DNA markers indicate unidirectional gene flow of Indo-Pacific to Atlantic bigeye tuna (Thunnus obesus) populations, and their admixture off southern Africa.2005

    • Author(s)
      Durand, J.-D.
    • Journal Title

      Marine Biology (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] PCR primers for fish G6PD gene intron and characterization of intron length variation in the albacore Thunnuns alalunga.2004

    • Author(s)
      Chow, S.
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 391-393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] 頭足類浮遊稚仔研究の現在.2004

    • Author(s)
      若林敏江
    • Journal Title

      海洋と生物 26

      Pages: 225-231

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] PCR primers for fish G6PD gene intron and characterization of itron length variation in the albacore Thunnuns alalunga2004

    • Author(s)
      Chow, S.
    • Journal Title

      Molecular Ecology Notes 4

      Pages: 391-393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Differing body size between the autumn and the winter-spring cohorts of neon flying squid (Ommastrephes bartramii) related to the oceanographic regime in the North Pacific : a hypotheis.2004

    • Author(s)
      Ichii, T.
    • Journal Title

      Fisheries Oceanography 13

      Pages: 295-309

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Interpretation of statolith microstructure in reared hatchling paralarvae of the squid Illex argentinus.2004

    • Author(s)
      Sakai, M.
    • Journal Title

      Marine and Freshwater Research 55

      Pages: 403-413

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Diving behavior of immature, feeding Pacific bluefin tuna (Thunnus thynnus orientalis) in relation to season and area : the East China Sea and the Kuroshio-Oyashio transition region.2004

    • Author(s)
      Kitagawa, T.
    • Journal Title

      Fisheries Oceanography 13

      Pages: 161-180

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 頭足類浮遊稚仔研究の現在2004

    • Author(s)
      若林敏江
    • Journal Title

      海洋と生物 26

      Pages: 225-231

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic and morphological identification of larval and small juvenile tunas (Pisces : Scombridae) caught by a mid-water trawl in the wstern Pacific.2003

    • Author(s)
      Chow, S.
    • Journal Title

      Bulletin of Fisheries Research Agency 8

      Pages: 1-14

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Genetic and morphological identification of larval and small juvenile tunas (Pisces : Scombridae) caught by a mid-water trawl in the western Pacific.2003

    • Author(s)
      Chow, S.
    • Journal Title

      Bulletin of Fisheries Research Agency 8

      Pages: 1-14

    • NAID

      40006149828

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Publications] Chow, S.et al.: "Genetic and morphological identification of larval and small juvenile tunas (Pisces : Scombridae) caught by a mid-water trawl in the western Pacific."Bulletin of Fisheries Research Agency. 8. 1-14 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirota, Y., Uehara, S., Honda, H.: "Ontogenetic changes of feeding selectivity in juvenile jack mackerel Trachurus japonicus collected off south-east Kyushu, Japan."Fisheries Science. 70(1). 100-107 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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