| Project/Area Number |
15380183
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Zootechnical science/Grassland science
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| Research Institution | Tohoku University |
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Principal Investigator |
KITAZAWA Haruki Tohoku University, Graduate School of Agricultural Science, Associate Professor, 大学院農学研究科, 助教授 (10204885)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Tadao Tohoku University, Graduate School of Agricultural Science, Professor, 大学院農学研究科, 教授 (00118358)
KAWAI Yasushi Tohoku University, Graduate School of Agricultural Science, Assistant Professor, 大学院農学研究科, 助手 (00261496)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2005: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 2003: ¥6,600,000 (Direct Cost: ¥6,600,000)
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| Keywords | Lactic acid bacteria / Probiotics / Immunobiotics / Toll-like receptor / DNA / AT-oligodeoxynucleotide / Peyer's patches / Mesenteric lymph nodes / Immunobiotics / AT oligonucleotide / 腸管膜リンパ節 / TLR9 / トランスフェクタント |
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| Research Abstract |
Recent interest has focused on the importance of intestinal immunity for the host defense, but to date, not much has been known about the underlying mechanisms. Toll-like receptor (TLR) family plays an important role in the defense through recognizing bacterial pathogen associated molecular patterns (PAMPs). In this study, the physiological function of food products has investigated the immunoregulatory effects of immunobiotic lactic acid bacteria (LAB) via TLRs. Studies in swine, which is expected as a human model, have been examined intestinal immunoregulation by the immunobiotic LAB. Our research has now demonstrated regulation of intestinal immunity mediated by TLRs in the gut. On the basis of this study, efforts have also been made to develop a molecular immunoassay system for immuobiotic LAB and find novel immunostimulatory DNA sequences from imunobiotics. In vitro and in vivo study demonstrated an anti-allergic activity of immunostimulatory DNA sequences from imunobiotics. The findings in this project may provide impetus to further delineate the activation mechanism of the innate immune response with immunostimulatory DNA. In addition, identification of immunoregulatory factors from LAB, and better understanding of intestinal immune regulation through cytokine networks hold promise in basic research of food immunology and development of "immunobiotic foods" to prevent allergic disease.
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