NIIZEKI Minoru Hirosaki University, Faculty of Agriculture and Life Sciences, Professor (40001490)
HARADA Takeo Hirosaki University, Faculty of Agriculture and Life Sciences, Professor (10228645)
AKADA Shinji Hirosaki University, Gene Research Center, Associate Professor (10250630)
ISHIKAWA Ryuji Hirosaki University, Faculty of Agriculture and Life Sciences, Associate Professor (90202978)
SENDA Mineo Hirosaki University, Faculty of Agriculture and Life Sciences, Associate Professor (30261457)
|Budget Amount *help
¥15,800,000 (Direct Cost : ¥15,800,000)
Fiscal Year 2006 : ¥3,900,000 (Direct Cost : ¥3,900,000)
Fiscal Year 2005 : ¥3,900,000 (Direct Cost : ¥3,900,000)
Fiscal Year 2004 : ¥3,900,000 (Direct Cost : ¥3,900,000)
Fiscal Year 2003 : ¥4,100,000 (Direct Cost : ¥4,100,000)
Beech forest is well conserved in a vast area of Shirakami-sanchi and the central part (1, 697 ha) of this area has been designated as the World Natural Heritage since 1993. In order to gain insights into how these forests would fare in the future when the global and the local climate changes might come forward, it is important to investigate intensively the genetic structure of the beech forest as well as the genetic capacity of the individual trees to cope with environmental changes. The achievements of this program have been summarized into the following 3 items.
1. Different types of DNA markers, such as SSR markers through molecular analysis of MYB family and the fragmented copies of IGS region, allowing detection of differences in individual genetic components, have been developed. In an attempt to develop some maternally inherited DNA markers, the beech chloroplast and mitochondrial DNA has been cloned and sequenced.
2. We established a plot for the inventory study in the area of
about 1.65 ha at Mt. Takakuramori, which is localized in the buffering area of the World Heritage, and analyzed the genetic markers of all the adult trees and saplings to investigate the genetic diversity of the forest as well as parentage of the saplings. We have also investigated the beech forest at the central area of the World Heritage, at Mt. Takakuma Kagoshima prefecture, and at Ogawa site Ibaraki prefecture, to find out the genetic distances of these forests and to carry out the comparative analysis of their genetic diversities.
3. In order to analyze the genetic capacity of individual beech trees, we investigated the cause for the beechnuts ending up empty, and found that there is a strong requirement for cross-pollination and self-incompatibility may be responsible for this. Furthermore, in order to identify some genes responsible for environmental adaptation and to investigate the genetic diversity of these genes, we analyzed the beech MYB gene family and identified, so far, 85 independent genes. Among these genes, we found out some genes responsive to various environmental stresses, such as, cold, high temperature, drought and also some genes whose expression fluctuates extensively depending on the seasons. Less