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Core histone modifications change the higher order of DNA structurec resulting in its biological output

Research Project

Project/Area Number15390098
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionNagasaki University

Principal Investigator

ITO Takashi  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (90306275)

Co-Investigator(Kenkyū-buntansha) IKEHARA Tsuyoshi  Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (90359951)
NAKAGAWA Takeya  Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (50363502)
YASUI Kiyoshi  Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (50372777)
Project Period (FY) 2003 – 2004
Project Status Completed(Fiscal Year 2004)
Budget Amount *help
¥14,700,000 (Direct Cost : ¥14,700,000)
Fiscal Year 2004 : ¥5,200,000 (Direct Cost : ¥5,200,000)
Fiscal Year 2003 : ¥9,500,000 (Direct Cost : ¥9,500,000)
Keywordshistone / acetylation / phosphorylation / nucleosome / chromatin / transcription
Research Abstract

In the eukaryotic nucleus, DNA exists as a nucleoprotein complex termed chromatin. The work is based on the hypothesis that chromatin dynamics and structure are an integral component of the analysis of DNA-dependent processes. Posttranslational histone modifications are important for the regulation of many biological phenomena. In this project, we clafified that a precise role for histone acetylation, namely to alter the structure of nucleosomes and facilitate the loss of H2A-H2B dimmers that have been previously remodeled by the action of ATP-dependent chromatin remodeling complexes. We also clarified that transcription from chromatin templates is ordered and sequential, with precise timing and roles for ATP-dependent chromatin remodeling, subsequent histone acetylation and alterations in nucleosome structure. In addition we found that histone H2A is phosphorylated by unique kinase. We found that NHK-1-catalyzed phosphorylation of a conserved serine/threonine residue in H2A is a new component of the histone code which might be related to cell cycle progression.

Report

(3results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

Research Products

(28results)

All 2004 2003 Other

All Journal Article Book Publications

  • [Journal Article] Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo.2004

    • Author(s)
      Aihara, H., Nakagawa, T.et al.
    • Journal Title

      Genes Dev 18

      Pages : 877-888

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Pitfall in Diagnosis of Human Prion Diseases Using Detection of Protease-resistant Prion Protein in Urine : CONTAMINATION WITH BACTERIAL OUTER MEMBRANE PROTEINS.2004

    • Author(s)
      Furukawa, H., Doh-Ura, K et al.
    • Journal Title

      J Biol Chem 279

      Pages : 23661-23667

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isoflavones stimulate estrogen receptor-mediated core histone acetylation.2004

    • Author(s)
      Hong, T., Nakagawa, T et al.
    • Journal Title

      Biochem Biophys Res Commun 317

      Pages : 259-264

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation.2004

    • Author(s)
      Mori, M., Yoneyama, M., Ito, T.et al.
    • Journal Title

      J Biol Chem 279

      Pages : 9698-9702

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo.2004

    • Author(s)
      Aihara, H., Nakagawa, T., Yasui, K., Ohta, T., Hirose, S., Dhomae, N., Takio, K., Kaneko, M., Takeshima, Y., Muramatsu, M., Ito, T.
    • Journal Title

      Genes Dev 18

      Pages : 877-888

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Pitfall in Diagnosis of Human Prion Diseases Using Detection of Protease-resistant Prion Protein in Urine : CONTAMINATION WITH BACTERIAL OUTER MEMBRANE PROTEINS.2004

    • Author(s)
      Furukawa, H., Doh-Ura, K., Okuwaki, R., Shirabe, S., Yamamoto, K., Udono, H., Ito, T., Katamine, S., Niwa, M.
    • Journal Title

      J Biol Chem 279

      Pages : 23661-23667

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Isoflavones stimulate estrogen receptor-mediated core histone acetylation.2004

    • Author(s)
      Hong, T., Nakagawa, T., Pan, W., Kim, M.Y., Kraus, W.L, Ikehara, T., Yasui, K., Aihara, H., Takebe, M., Muramatsu, M., Ito, T.
    • Journal Title

      Biochem Biophys Res Commun 317

      Pages : 259-264

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation.2004

    • Author(s)
      Mori, M., Yoneyama, M., Ito, T., Takahashi, K., Inagaki, F., Fujita, T.
    • Journal Title

      J Biol Chem 279

      Pages : 9698-9702

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo.2004

    • Author(s)
      Aihara, H., Nakagawa, T., Yasui, K., Ohta, T., Hirose, S., Dhomae, N., Takio, K., Kaneko, M., Takeshima, Y., Muramatsu, M., Ito, T
    • Journal Title

      Genes Dev 18

      Pages : 877-888

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A Pitfall in Diagnosis of Human Prion Diseases Using Detection of Protease-resistant Prion Protein in Urine : CONTAMINATION WITH BACTERIAL OUTER MEMBRANE PROTEIN2004

    • Author(s)
      Furukawa, H., Doh-Uta, K., Okuwaki, R., Shirabe, S., Yamamoto, K, Udono, H., Ito, T., Katamine, S., Niwa, M.
    • Journal Title

      J Biol Chem 279

      Pages : 23661-23667

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Isfolavones stimulate estrogen receptor-mediated core histone acetylation2004

    • Author(s)
      Hong, T., Nakagawa, T., Pan, W., Kim, M.Y., Kraus, W.L., Ikehara, T., Yasui, K., Aihara, H., Takebe, M., Muramatsu, M., Ito, T.
    • Journal Title

      Biochem Biophys Res Commun 317

      Pages : 259-264

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation.2004

    • Author(s)
      Mori., M., Yoneyama., M., Ito., T., Takahashi., K., Inagaki., F., Fujita., T.
    • Journal Title

      J Biol Chem 279

      Pages : 9698-9702

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome.2003

    • Author(s)
      Kitagawa, H., Fujiki, R., Yoshimura, K., Mezaki, Y., Uematsu, Y., Matsui, D., Ogawa, S., Unno, K., Okubo, M., Tokita, A., Nakagawa, T., Ito, T.et al.
    • Journal Title

      Cell 113

      Pages : 905-917

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nucleosome assembly and remodeling2003

    • Author(s)
      Ito, T
    • Journal Title

      Curr Top Microbiol Immunol 274

      Pages : 1-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 蛋白質複合体によるクロマチン構造変換2003

    • Author(s)
      伊藤 敬
    • Journal Title

      実験医学 21

      Pages : 1398-1401

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] クロマチンの形成と再構築2003

    • Author(s)
      安井 潔, 伊藤 敬
    • Journal Title

      実験医学 21

      Pages : 1402-1407

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] シグナル伝達とクロマチン制御2003

    • Author(s)
      中川 武弥, 伊藤 敬
    • Journal Title

      蛋白質核酸酵素 48

      Pages : 2234-2240

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome.2003

    • Author(s)
      Kitagawa, H., Fujiki, R., Yoshimura, K., Mezaki, Y., Uematsu, Y., Matsui, D., Ogawa, S., Unno, K., Okubo, M., Tokita, A., Nakagawa, T., Ito, T., Ishimi, Y., Nagasawa, H., Matsumoto, T., Yanagisawa, J., Kato, S.
    • Journal Title

      Cell 113

      Pages : 905-917

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nucleosome assembly and remodeling.2003

    • Author(s)
      Ito, T.
    • Journal Title

      Curr Top Microbiol Immunol 274

      Pages : 1-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Protein complexes that affect chromatin structure2003

    • Author(s)
      Ito, T.
    • Journal Title

      Jikkenigaku 21

      Pages : 1398-1401

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chromatin assembly and remodeling2003

    • Author(s)
      Yasui, K., Ito, T.
    • Journal Title

      Jikkenigaku 21

      Pages : 1402-1407

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Signal transduction and chromatin2003

    • Author(s)
      Nakagawa, T., Ito, T.
    • Journal Title

      Tanpakushitu Kakusan Koso 48

      Pages : 2234-2240

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] ショウジョウバエ胚抽出液によるクロマチン形成法2004

    • Author(s)
      分担執筆 伊藤 敬
    • Total Pages
      231
    • Publisher
      羊土社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] クロマチン染色体実験プロトコール ショウジョウバエ胚抽出液によるクロマチン形成法2004

    • Author(s)
      押村光雄, 平岡泰 編, 分担執筆 伊藤 敬
    • Total Pages
      98-104
    • Related Report
      2004 Annual Research Report
  • [Book] 新ラボマニュアル遺伝子工学2003

    • Author(s)
      分担執筆 池原強, 中川武弥, 伊藤 敬
    • Total Pages
      291
    • Publisher
      丸善株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Mori, M., Yoneyama, M., Ito, T.et al.: "Identification of ser-386 of interferon regulatory factor 3 as critical target for inducible phosphorylation that determines activation"J Biol Chem. 279. 9698-9702 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kitagawa, H., Fujiki, R., Yoshimura, K., Mezaki, Y., Uematsu, Y., Matsui, D., Ogawa, S., Unno, K., Okubo, M., Tokita, A., Nakagawa, T., Ito, T.et al.: "The chromatin-remodeling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome"Cell. 113. 905-917 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ito, T: "Nucleosome assembly and remodeling"Curr Top Microbiol Immunol. 274. 1-22 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2003-04-01   Modified : 2016-04-21  

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