Mechanisms of formation of cell-cell junctions
| Project/Area Number |
15390099
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kumamoto University |
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Principal Investigator |
NAKANISHI Hiroyuki Kumamoto University, Graduate School of Medical Sciences, Professor, 大学院・医学薬学研究部, 教授 (80314318)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 2004: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 2003: ¥8,300,000 (Direct Cost: ¥8,300,000)
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| Keywords | cell-cell adhesion / nectin / afadin / cadherin / epithelial cells / adherens Junctions / commissural axons / floor plate / ドミナント・ネガティブ変異体 / タイト・ジャンクション |
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| Research Abstract |
We have found a novel cell-cell adhesion system consisting of nectin, an immunoglobulin-like adhesion molecule, and afadin, an F-actin-binding protein. This nectin-afadin system organizes adherens and tight junctions in epithelial cells. During the support from 2003 to 2004, we have studied the functions and mode of action of the nectin-afadin system. The results obtained are as follows :
1.A fragment of N-cadherin lacking its extracellular region serves as a dominant negative mutant(DN) and inhibits cell-cell adhesion activity of E-cadherin, but its mode of action remains to be elucidated. We have suggested that N-cadherin DN competitively inhibits the association of the endogenous nectin-afadin system with the endogenous E-cadherin-catenin system and thereby reduces the cell-cell adhesion activity of E-cadherin.
2.Nectin is necessary and sufficient for the recruitment of E-cadherin to the nectin-based cell-cell adhesion sites and involved in the formation of E-cadherin-based adherens junctions.
3.The nectin-afadin system is involved in dynamic epithelial remodeling during mouse development.
4.The contacts between the commissural axons and the floor plate cells are mediated by the hetero-trans-interaction between nectin-1 and nectin-3 and involved in regulation of the trajectory of the commissural axons.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] Contacts between the commissural axons and the floor plate cells are mediated by nectins.2004
Author(s)
Okabe, N., Shimizu, K., Ozaki-Kuroda, K., Nakanishi, H., Morimoto, K., Takeuchi, M., Katsumaru, H., Murakami, F., Takai, Y.
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Journal Title
Dev.Biol. 273
Pages: 244-256
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Antagonistic and agonistic effects of an extracellular fragment of nectin on formation of E-cadherin-based cell-cell adhesion.2003
Author(s)
Honda, T., Shimizu, K., Kawakatsu, T., Yasumi, M., Shingai, T., Fukuhara, A., Inagaki, M., Ozaki-Kuroda, K., Irie, K., Nakanishi, H., Takai, Y.
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Journal Title
Genes Cells 8
Pages: 51-63
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of nectin in formation of E-cadherin-based adherens junctions in keratinocytes---Analysis with the N-cadherin dominant negative mutant.2003
Author(s)
Tanaka, Y., Nakanishi, H., Kakunaga, S., Okabe, N., Kawakatsu, T., Shimizu, K., Takai, Y.
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Journal Title
Mol.Biol.Cell 14
Pages: 1597-1609
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The expression of nectin-1a in normal human skin and various skin tumors.2003
Author(s)
Matsushima, H., Utani, A., Endo, H., Matsuura, H., Kakuta, M., Nakamura, Y., Matsuyoshi, N., Matsui, C., Nakanishi, H., Takai, Y., Shinkai, H.
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Journal Title
Eur.J.Dermatol. 148
Pages: 755-762
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A novel rabconnectin-binding protein that directly binds a GDP/GTP exchange protein for the Rab3A small G protein implicated in Ca^<2+>-dependent exocytosis of neurotransmitter.2003
Author(s)
Kawabe, H., Sakisaka, T., Yasumi, M., Shingai, T., Izumi, G., Nagano, F., Deguchi-Tawarada, M., Takeuchi, M., Nakanishi, H., Takai, Y.
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Journal Title
Genes Cells 8
Pages: 537-546
Description
「研究成果報告書概要(欧文)」より
Related Report
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