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Establishment of array-based CGH technology and its use for-diagnosis of genetic diseases in clinical setting

Research Project

Project/Area Number 15390112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human genetics
Research InstitutionTokyo Medical and Dental University

Principal Investigator

INAZAWA Johji  Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Professor, 難治疾患研究所, 教授 (30193551)

Co-Investigator(Kenkyū-buntansha) IMOTO Issei  Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Associate Professor, 難治疾患研究所, 助教授 (30258610)
YOKOI Sana  Tokyo Medical and Dental University, Medical Research Institute, Department of Molecular Cytogenetics, Research Associate, 難治疾患研究所, 助手 (30372452)
MIZUTANI Syuki  Tokyo Medical and Dental University, Graduate School, Pediatrics and Developmental Biology, Professor, 大学院・医歯学総合研究科, 教授 (60126175)
ARII Shigeki  Tokyo Medical and Dental University, Graduate School, Hepato-Biliary Pancreatic Surgery, Professor, 大学院・医歯学総合研究科, 教授 (50151171)
斎藤 深美子  東京医科歯科大学, 難治疾患研究所, 助手 (10158917)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥15,900,000 (Direct Cost: ¥15,900,000)
Fiscal Year 2004: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 2003: ¥9,600,000 (Direct Cost: ¥9,600,000)
KeywordsCGH / Genetic disease / Chromosome aberration / BAC / PAC / DNA methylation / Autism / contiguous gene syndrome
Research Abstract

Comparative genomic hybridization (CGH) has already made a significant impact on cancer cytogenetics. However, CGH to metaphase chromosomes can provide only limited resolution at 5-10 Mb level. To circumvent this limitation, array-based CGH has been devised. We have constructed different types of BAC-based CGH-arrays. The first consisted of 〜4500 BACs for genome-wide analysis (named MCG Whole Genome Array-4500) ; this array provided a resolution of 〜0.7Mb. The second consisted of 〜800 BACs harboring 800 known cancer-relaed genes, intended for diagnosis of cancer-specific copy-number aberrations (MCG Cancer Array-800, see URL : http://www.cghtmd.jp/cghdatabase/index.html). The third of our arrays contains 212 contiguous BACs spanning a 〜20-Mb region at 1p36 (MCG 1p36 Contig Array). Furthermore, to accomplish high-throughput screening for methylated sites in the whole genome, we have combined array-CGH with MCA, and our preliminary data show that this "BAC array-based MCA (BAMCA)" can discriminate BAC clones that harbor methylated CpGs on an array platform. Our custom-made CGH-arrays paves the way for identification of disease-related genetic aberrations that have not yet been detected by existing technologies, and our array-based CGH technology should soon be practical for diagnosis of cancer or genetic diseases in clinical settings.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2004 Other

All Journal Article (9 results) Book (1 results) Patent(Industrial Property Rights) (2 results) Publications (7 results)

  • [Journal Article] Alteration in copy numbers of genes as a mechanism for acquired drug resistance2004

    • Author(s)
      Yasui K, Inazawa J et al.
    • Journal Title

      Cancer Research 64

      Pages: 1403-1410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Frequent silencing of low density lipoprotein receptor-related protein1B(LRP1B) expression by genetic and epigenetic mechanisms in esophageal squamous-cell carcinoma2004

    • Author(s)
      Sonoda I, Inazawa J et al.
    • Journal Title

      Cancer Research 64

      Pages: 741-747

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Skp2 Overexpression is a p27Kip1-independent poor prognosticator in patients with biliary tract cancers2004

    • Author(s)
      Sanada T, Inazawa J et al.
    • Journal Title

      Cancer Science 95

      Pages: 969-976

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Comparative genomic hybridization (CGH) arrays pave the way for identification of novel cancer-related genes2004

    • Author(s)
      Inazawa J et al.
    • Journal Title

      Cancer Science 95

      Pages: 559-563

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Amplification and Overexpression of SKP2 Are Associated with Metastasis of Non-Small-Cell Lung Cancers to Lymph Nodes2004

    • Author(s)
      Yokoi S, Inazawa J et al.
    • Journal Title

      Am J Pathol 165

      Pages: 175-180

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Overexpression of PDZK1 within the 1q12-q22 Amplicon Is Likely To Be Associated with Drug-Resistance Phenotype in Multiple Myeloma2004

    • Author(s)
      Inoue J, Inazawa J et al.
    • Journal Title

      Am J Pathol 165

      Pages: 71-81

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Skp2 overexpression is a p27Kip1-independent poor prognosticator in patients with biliary tract cancers2004

    • Author(s)
      Sanada T, Inazawa J et al.
    • Journal Title

      Cancer Science 95

      Pages: 969-976

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Frequent silencing of low density lipoprotein receptor-related protein 1B(LRP1B) expression by genetic and epigenetic mechanisms in esophageal squamous-cell carcinoma2004

    • Author(s)
      Sonoda I, Inazawa J et al.
    • Journal Title

      Cancer Research 64

      Pages: 741-747

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Overexpression of PDZK1 within the 1q12-q22 Amplicon Is Likely To Be Associated with Drug-Resistance Phenotype in Multiple Myeloma.2004

    • Author(s)
      Inoue J, Inazawa J et al.
    • Journal Title

      Am J Pathol 165

      Pages: 71-81

    • Related Report
      2004 Annual Research Report
  • [Book] 学術月報2004

    • Author(s)
      稲澤 譲治
    • Total Pages
      88
    • Publisher
      独立行政法人日本学術振興会
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 薬剤耐性を獲得した癌細胞の検出方法2004

    • Inventor(s)
      稲澤 譲治, 安居 幸一郎
    • Filing Date
      2004-02-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 薬剤耐性を獲得した癌細胞の検出方法2004

    • Inventor(s)
      稲澤譲治, 安居幸一郎
    • Filing Date
      2004-02-04
    • Related Report
      2004 Annual Research Report
  • [Publications] Yasui K, Inazawa J et al.: "Alteration in Copy-Numbers of Genes as a Mechanism for Acquired Drug Resistance"Cancer Research. 64. 1403-1410 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirasawa A, Inazawa J et al.: "Relationship between Prognostic Factors and the Biological Mechanisms Accounting for Genetic Instability in Endometrial Adenocarcinoma of the Uterus"Clinical Cancer Research. 15. 5675-5682 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Okamoto H, Inazawa J et al.: "PTK2 and EIF3S3 Genes May be Amplification Targets at 8q23-q24 and Are Associated with Large Hepatocellular Carcinomas"Hepatology. 38. 1242-1249 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Imoto I, Inazawa J et al.: "Identification of ZASC1 Encoding a Kruppel-like Zinc Finger Protein as a Novel Target for 3q26 Amplification in Esophageal Squamous Cell Carcinomas"Cancer Research. 63. 5691-5696 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Yokoi S, Inazawa J et al.: "TERC Identified as a probable target within the 3q26 amplicon that is detected frequently in non-small cell lung cancers"Clinical Cancer Research. 15. 4705-4713 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hirasawa A, Inazawa J et al.: "Association of 17q21-q24 Gain in Ovarian Clear Cell Adenocarcinomas with Poor Prognosis and Identification of PPM1D and APPBP2 as Likely Amplification Targets"Clinical Cancer Research. 9. 1995-2004 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 稲澤譲治: "生命科学"東京化学同人. 228 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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