A Role of somatic stem cells in pathogenesis and treatments of hereditary hepatic disorders.
| Project/Area Number |
15390113
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human genetics
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| Research Institution | Kumamoto University |
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Principal Investigator |
ENDO Fumio Kumamoto University, School of Medicine Department of Pediatrics, Professor, 大学院・医学薬学研究部, 教授 (00176801)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Tetsuro Kumamoto University, School of Medicine Department of Pediatrics, Professor, 大学院・医学薬学研究部, 教授 (60112405)
ADACHI Naoto Kumamoto University, School of Medicine Department of Hospital, Lecturer, 大学院・医学薬学研究部, 助教授 (00264292)
NAKAMURA Kimitoshi Kumamoto University, School of Medicine Department of Hospital, Assistant, 医学部附属病院, 助手 (30336234)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 2004: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 2003: ¥9,500,000 (Direct Cost: ¥9,500,000)
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| Keywords | liver failure / amino acid metabolism / enzyme defect / cancer / mouse model / apoptosis / 遺伝性疾患 |
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| Research Abstract |
Researches on pathogenesis and quest for new treatments require approaches from vew view points. For treatment of liver disease, employment of stam cell systems are believed to be hopeful. Induction of endodermal cells such as liver cells or pancreatic cells, from ES cells or somatic stem cells.
(1)In the present study, we extended previous investigations on the gene expression during the process of cell injury.
(2)Hereditary tyrosinemia type 1, a genetic disease caused by a deficiency of fumarylacetoacetate hydrolase(FAH), induces severe visceral injuries. Mice with FAH deficiency are lethal after birth ; thus, efforts to elucidate the mechanisms of the disease process have been impeded. The use of Fah(-/-) Hpd(-/-) double-mutant mice has enabled studies on tyrosinemias, and essential features of visceral injury have been revealed. In this mice models, we transplanted endodermal somatic stem cells isolated from salivary glands. Two weeks after, the livers Were dissected and analysed for repopulation. The results indicated 20-40% of hepatic cells are replaced by transplqabted cells.
The results presented here suggested that endodermal stem cells will represent the endodermal stem cells of adults.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages.2003
Author(s)
Okumura K., Nakamura K., Hisatomi Y., Nagano K., Tanaka Y., Terada K., Sugiyama T., Umeyama K., Matsumoto K., Yamamoto T., Endo F.
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Journal Title
hepatology 38
Pages: 105-113
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A missense mutation(GGC[^<435>Gly→AGC[Ser]] in exon 8 of the CYP11B2 gene inherited in Japanese Patients with Congenital hypoaldosteronism.2003
Author(s)
Kuribayashi I., Kuge H., Santa J.R., Mutlaq Z.A., Yamasaki N., Furuno T., Takahashi A., Chida S., Nakamura T., Endo F., Doi Y., Onishi S., Shizuta Y.
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Journal Title
Horm.Res 60
Pages: 255-260
Description
「研究成果報告書概要(欧文)」より
Related Report
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