Analysis on porcine immune system and generation of immunodeficient pig for the application to regeneration medicine

• Project/Area Number: 15390133
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: FUJIMOTO Junichiro National Research Institute for Child Health and Development, Vice General Director, 副所長 (60175578)
• Co-Investigator(Kenkyū-buntansha): KIYOKAWA Nobutaka National Research Institute for Child Health and Development, Department of Developmental Biology, Director, 発生・分化研究部, 部長 (60195401) ; YAMADA Taketo Keio University, School of Medicine, Department of Pathology, Lecturer, 医学部, 専任講師 (60230463) ; YASUE Hiroshi National Institute of Agrobiological Sciences, Genome Research Group, Senior Investigator, ゲノム研究グループ, 上席研究官 (80113497)
• Project Period (FY): 2003 – 2005
• Project Status: Completed (Fiscal Year 2005)
• Budget Amount: ¥13,200,000 (Direct Cost: ¥13,200,000); Fiscal Year 2005: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000); Fiscal Year 2003: ¥6,800,000 (Direct Cost: ¥6,800,000)
• Keywords: Immunodeficient pig / Regenerative medicine / cDNA library / porcine T-cell / MHC / TCR / 腫瘍組織適合抗原

Research Abstract

The monoclonal antibody 7G3 against the framework of porcine T-cell receptor gamma chain was established. When compared with previously established other antibody against the same molecule, it was found 7G3 worked better than others in terms of strength of reactivity on cells and tissue sections as well as stability in immunobiochemical analysis. In order to understand the mechanism involved in porcine TCR gene editing, TCR Ja chain was analyzed and it was found that usage of Ja gene segment was very similar to that of human system. In addition, variability of CDR3 region of porcine TCR alpha cDNA was investigated and it was found that average length was 9.35 (average length in human system is 10.2) and that at least 33 porcine TCR alpha genes were identified.

Research Products

• [Journal Article] Genomic structure of swine taste receptor family 1 member 3, TASIR3, and its expression in tissues. (2006)
  • Author(s): Kiuchi S, Yamada T, Kiyokawa N, Saito T, Fujimoto J, H Yasue.
  • Journal Title: Cytogenetic and Genome Research (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues. (2006)
  • Author(s): Kiuchi S, Yamada T, Kiyokawa N, Saito T, Fujimoto J, H Yasue.
  • Journal Title: Cytogenetic and Genome Research (印刷中)
• [Journal Article] Characterization of new monoclonal antibodies against porcine lymphocytes molecular characterization of clone 7G3, an antibody reactive with the constant region of the T-cell receptor d-chains. (2005)
  • Author(s): Tang W-R, Shioya N, Eguchi T, Ebata T, Matsui J, Takenouchi H, Honma D, Yasue H, Takagaki Y, Enosawa S, Itagaki M, Taguchi T, Kiyokawa N, Amemiya H, Fuiimoto J.
  • Journal Title: Veterinary Immunol Immunopathol 103 Pages: 113-127
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Porcine TCR CD3zeta-chain and eta-chain. (2005)
  • Author(s): Yamamoto R, Isobe T, Eguchi T, Tang WR, Kiyokawa N, Amemiya H, Fujimoto J, Sato E, Takagaki Y, Yasue H.
  • Journal Title: Mol Immunol 42(12) Pages: 1485-1493
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of new monoclonal antibodies against porcine lymphocytes : molecular characterization of clone 7G3, an antibody reactive with the constant region of the T-cell receptor d-chains. (2005)
  • Author(s): Tang W-R, Shioya N, Eguchi T, Ebata T, Matsui J, Takenouchi H, Honma D, Yasue H, Takagaki Y, Enosawa S, Itagaki M, Taguchi T, Kiyokawa N, Amemiya H, Fuijmoto J.
  • Journal Title: Veterinary Immunol Immunopathol 103 Pages: 113-127
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Porcine TCR CD3zeta-chain and eta-chain. (2005)
  • Author(s): Yamamoto R, Isobe T, Eguchi T, Tang WR, Kiyokawa N, Amemiya H, Fujimoto J, Sato E, Takagaki Y, Yasue H.
  • Journal Title: Mol Immunol 42 Pages: 1485-1493
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of new monoclonal antibodies against porcine lymphocytes : molecular characterization of clone 7G3,an antibody reactive with the constant region of the T-cell receptor δ-chains. (2005)
  • Author(s): Tang W-R, Shioya N, Eguchi T, Ebata T, Matsui J, Takenouchi H, Honma D, Yasue H, Takagaki Y, Enosawa S, Itagaki M, Taguchi T, Kiyokawa N, Amemiya H, Fujimoto J.
  • Journal Title: Veterinary Immunol Immunopathol (in press)
• [Journal Article] Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain. (2004)
  • Author(s): Tang W, Kiyokawa N, Eguchi T, Matsui J, Takenouchi H, Nonma D, Yasue H, Enosawa S, Mimori K, Itagaki M, Taguchi T, Katagiri YU, Okita H, Amemiya H, Fujimoto J.
  • Journal Title: Hybridoma and Hybridomics 23 Pages: 187-191
• [Journal Article] Nucleotide sequencing analysis of the swine 433-kb genomic segment located between the non-classical and classical SLA class I gene clusters. (2004)
  • Author(s): Shigenari A, Ando A, Renard C, Chardon P, Shiina T, Kulski JK, Yasue H, Inoko H.
  • Journal Title: Immunogenetics. 55 Pages: 695-705
• [Journal Article] Trophinin is expressed in the porcine endometrium during the estrous cycle. (2004)
  • Author(s): Nakano S, Kishi H, Ogawa H, Yasue H, Okano A, Okuda K.
  • Journal Title: J Reprod Dev 49 Pages: 127-134
  • NAID: 10026618781
• [Journal Article] Analysis of a full-length cDNA library constructed from swine olfactory bulb for elucidation of expressed genes and their transcription initiation sites. (2004)
  • Author(s): Fujisaki S, Sugiyama A, Eguchi T, Watanabe Y, Hiraiwa H, Honma D, Saito T, Yasue H.
  • Journal Title: J Vet Med Sci 66 Pages: 15-23
  • NAID: 130000448805
• [Journal Article] Genomic structure of swine taste receptor family 1 member 3, TAS1R3, and its expression in tissues.
  • Author(s): Kiuchi S, Yamada T, Kiyokawa N, Saito T, Fujimoto J, H Yasue.
  • Journal Title: Cytogenetic and Genome Research (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tang W, Kiyokawa N, Eguchi T, Matsui J, Takenouchi H, Nonma D, Yasue H, Enosawa S, Mimori K, Itagaki M, Taguchi T, Katagiri YU, Okita H, Amemiya H, Fujimoto J: "Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain."Hybridoma and Hybridomics. 印刷中. (2004)
• [Publications] Honma D, Uenishi H, Hiraiwa H, Watanabe S, Tang W, Kiyokawa N, Fujimoto J, Yasue H, Sakimura K: "Cloning and characterization of porcine common gamma chain gene."J Interferon Cytokine Res.. 23(2). 101-111 (2003)
• [Publications] Taguchi T, Kiyokawa N, Mimori K, Suzuki T, Sekino T, Nakajima H, Saito M, Katagiri-U Y, Matsuo N, Matsuo Y, Karasuyama H, Fujimoto J: "Pre-BCR-mediated signal inhibits CD24-induced apoptosis in human pre-B cells."J Immunol. 170(1). 252-260 (2003)
• [Publications] Uenishi H, Hiraiwa H, Yamamoto R, Yasue H, Takagaki Y, Shiina T, Kikkawa E, Inoko H, Awata T.: "Genomic structure around joining segments and constant regions of swine T-cell receptor alpha/delta (TRA/TRD) locus."Immunology. 109(4). 515-526 (2003)
• [Publications] Fujisaki S, Sugiyama A, Eguchi T, Watanabe Y, Hiraiwa H, Honma D, Saito T, Yasue H: "Analysis of a full-length cDNA library constructed from swine olfactory bulb for elucidation of expressed genes and their transcription initiation sites."J Vet Med Sci. 印刷中. (2004)