| Project/Area Number |
15390363
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
FUJII Hirotada Sapporo Medical University, School of Health Sciences, Professor, 保健医療学部, 教授 (70209013)
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| Co-Investigator(Kenkyū-buntansha) |
KOSE Katsumi Tsukuba University, Department of Physics, Professor, 物理工学系, 教授 (60186690)
AOKI Masaaki NEOMAX co.LTD., マグネット応用開発部, 研究員
HAISHI Tomoyuki MR Technology Inc., 研究員
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥13,100,000 (Direct Cost: ¥13,100,000)
Fiscal Year 2004: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥10,000,000 (Direct Cost: ¥10,000,000)
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| Keywords | visualization / MRI / ESR / active oxygen / free radical / superoxide / 活性酵素 / 画像化 |
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| Research Abstract |
Electron Paramagnetic Resonance Imaging (EPRI) technique has been developed to obtain the spatial distribution of the paramagnetic materials, particularly on a microscopic scale. A large number of biological studies were successfully carried out, especially for the visualization of bio-radicals generated in in vivo system. Typical example is the detection and visualization of nitric oxide (NO) in septic-shocked animals. The strength of EPRI is that only free radicals are visible in its image. On the contrary, weakness of EPRI is that nothing can be visible except paramagnetic materials on its image. Therefore, it seems very hard to obtain in which organs the bioradicals were generated in the small animals. In order to overcome this problem, there are three possible methods to visualize free radicals on the anatomical map of the object. 1: PEDRI (Proton Electron Double Resonance Imaging), 2: conventional MRI, and 3: EPR+MRI system. In this study, we have tried developing EPR/MRI dual imaging system to visualize free radicals on the anatomical map of the object.
EPR/MRI dual imaging system was constructed by using the permanent magnet (Nb-Fe-B material) rather than electromagnets or superconductive magnets because it is easier to assemble and combine EPR and MRI components. EPR spectrometer in this dual imaging system is working at 1.2 GHz at 0.04T, and MRI spectrometer in this dual system is working at 22 MHz at 0.5T. The most surprising feature is that the center of MRI magnet is only 20 cm apart from that of EPR magnet. Each spectrometer is working well without any magnetic or electric interference.
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