| Project/Area Number |
15390402
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
AIKOU Takashi Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (60117471)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAO Sonsin Kagoshima University, Frontier Science Research Center, Professor, フロンティアサイエンス研究推進センター, 教授 (80171411)
NAKAJYO Masayuki Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (20041379)
NATSUGOE Shouji Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70237577)
NAKAJYO Akihiro University Hospital, Faculty of Medicine and Dentistry, Research Associate, 医学部・歯学部附属病院, 助手 (20404486)
帆北 修一 鹿児島大学, 医学部・歯学部附属病院, 講師 (60274833)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥10,700,000 (Direct Cost: ¥10,700,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2003: ¥6,800,000 (Direct Cost: ¥6,800,000)
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| Keywords | Sentinel Node / Lymph node metastasis / Micro-metastasis / Immuno-histochemistry / GI cancer / Lymphadenectomy / Immuno-response / early cancer / センチネルリンパ節 / Cytokeratin / RT-PCR法 / 増殖活性能 / Sentinel Node Navigation / CEA-mRNA / Micrometastasis / Isolated Tumor cell / 見張りリンパ節 / ナビゲーション手術 / リンパ転移 / 縮小手術 |
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| Research Abstract |
One of the breakthroughs in surgical oncology is that the sentinel lymph node (SN) procedure has opened a window to clinical practice in the field of Gastro-Intestinal (GI) surgery. Our interest is in whether the SN concept is acceptable in GI cancer surgery. SN may be the first lymph node which undergoes influence of factors produced by primary lesion and the initial site of antigen presentation associated with immune responses. In this study, we investigated the biologic features of metastasis including micro-metastasis in SN and evaluated sentinel node concept using both dye and radioisotope in patients with GI cancer.
One day prior to surgery, ^<99m>Tc-tin colloid was endoscopically injected into the sub- mucosa near the tumor. Radioisotope uptake in all dissected lymph nodes was measured during and after surgery. Lymph node metastasis and micro-metastasis were examined by RT-PCR using CEA mRNA in addition to Hematoxylin-Eosin staining (HE) and immunohistochemistry (IHC).
As a result, sentinel node navigation surgery appears to be clinically acceptable only in early stage of disease that is patients with clinical mucosal or sub-mucosal tumor. A reduction of lymphadenectomy based on sentinel node detection is indicated for such patients. Local immune response in SN with metastasis showed various immune statuses in comparison with non-SN. Although SN concept appear to be acceptable even for GI cancer, the standard procedure and criteria for SN is essential to further improve its clinical application. Another urgent task is to establish a standard method for detection micro-metastasis in SN.
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