| Project/Area Number |
15390432
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
YAMASHIMA Tetsumori Kanazawa Univ., Graduate School of Medical Science, Dept.of Restorative Neurosurgery, Associate Professor, 大学院・医学系研究科, 助教授 (60135077)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Satoshi Kanazawa Univ., Graduate School of Medical Science, Dept.of Neuroanatomy, Professor, 大学院・医学系研究科, 教授 (90283746)
向田 直史 金沢大学, がん研究所, 教授 (30182067)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥11,300,000 (Direct Cost: ¥11,300,000)
Fiscal Year 2005: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2004: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 2003: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | neurogenesis / monkey / hippocampus / cerebral ischemia / neural progenitor / subgranular zone / dentate gyrus / adventitia / 一過性脳虚血 / 神経再生 / 神経幹細胞 / BDNF / PSA-NCAM / 歯状回 / CA1 / 神経細胞死 / アポトーシス / ネクローシス / リソソーム / カルパイン / カテプシン |
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| Research Abstract |
The followings are main findings of the past three years, using a model of cerebral injury-global ischemia.
1)We found that ischemia can increase the number of NPC in adult monkey hippocampus similarly to the rodent brain, but the monkey response was much lower than the rodent response-10 times in quantity, and more importantly-15 times in ability to produce neurons (neuronal differentiation) (Mol Cell Neurosci 23:292-301,2003).
2)Further, monkey NPC were unable to replace any neurons in the ischemia-vulnerable CA1 sector (Glia 42;209-224,2003), which suggests that they cannot mediate a clinically-significant effect without an external influence.
3)Recently, we have identified potential molecular explanation for this discrepancy between rodent and monkey NPC-while rodent NPC in CA1 sector express the developmental transcription factors Pax6,Emx2 and Ngn2 that can promote neurogenesis, monkey NPC in CA1 did not express these proteins.
4)Importantly, we have identified a vascular niche around the adventitia of blood vessels that can promote neurogenesis in monkey dentate gyrus but not in CA1 sector (Hippocampus 14:861-875,2004).
5)Lastly, we were the first to describe postischemic NPC upregulation in SVZ of the lateral ventricle, also in the olfactory bulb, and a limited neuronal production in the postischemic monkey neocortex and striatum (J Neurosci Res 81:776-788,2005).
6)Thus, the applicant's group has investigated all major regions in the primate telencephalon with respect to postischemic neurogenesis.
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