Donor vascular endothelial regeneration and chimerism induced by recipient bone marrow transplantation
| Project/Area Number |
15390486
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Niigata University |
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Principal Investigator |
SAITO Kazuhide Niigata University, Medical and Dental Hospital, Lecturer, 医歯学総合病院, 講師 (20262438)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kota Niigata University, Institute of Medicine and Dentistry, Professor, 医歯学系, 教授 (90101857)
OITE Takashi Niigata University, Institute of Medicine and Dentistry, Professor, 医歯学系, 教授 (60018744)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥12,600,000 (Direct Cost: ¥12,600,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2004: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2003: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | microcirculation injury / ischemia-reperfusion injury / renal vascular endothelial cells / rat bone marrow transplantation / GFP positive cells / stem cell transplantation / 腎虚血再灌流障害 / 血管内皮細胞 / シクロスポリン / 骨髄幹細胞 / 腎虚血灌流障害 / 血管内皮細胞障害 / 単離腎潅流実験系 / 実時間型生体顕微鏡 / 免疫組織学 |
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| Research Abstract |
1. From the clinicopathological observation of antibody-mediated rejection in living donor ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation, We had analyzed and evaluated the process of the microcirculation injury and microvascular endothelial-cell damage.
2. We had established the GFP(+) to (-) rat bone marrow transplantation model and evaluated the role of The bone marrow derived endothelial progenitor cells in the regeneration of the injured microvascular Cells in renal ischemia-reperfusion injury.
3. We have revealed that the bone marrow derived endothelial cells have migrated and recovered the injured endothelial cells in renal ischemia-reperfusion injury. It was approved not only by static immunohinstopathological-method but also by dynamic-direct observation by real-time phase contrast microscopy.
4. Our result have approved for the first time that the injured renal microvascular endothelial cells were restored by Bone-marrow derived cells and reconstructed functional microvasculature.
These result will be useful for the clinical renal preservation and regeneration therapy for antibody-mediated rejection in ABO-incompatible kidney transplantation and ischemia-reperfusion injury in deceased donor kidney transplantation.
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Report
(5 results)
Research Products
(31 results)
