| Project/Area Number |
15390493
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NISHITANI Masa-aki (2004) The University of Tokushima, the medical Department, Associate Professor, 大学院・ヘルスバイオサイエンス研究部, 助教授 (40304521)
香川 征 (2003) 徳島大学, 医学部, 教授 (40035738)
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| Co-Investigator(Kenkyū-buntansha) |
KANAYAMA Hiro-omi The University of Tokushima, the medical Department, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (10214446)
TAKAHASHI Masayuki The University of Tokushima, the hospital Attached to the medical department, Lecturer, 医学部・歯学部附属病院, 講師 (50325255)
西谷 真明 徳島大学, 医学部・歯学部附属病院, 講師 (40304521)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 2004: ¥4,700,000 (Direct Cost: ¥4,700,000)
Fiscal Year 2003: ¥10,300,000 (Direct Cost: ¥10,300,000)
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| Keywords | Genitourinary cancer / Interstitial cells / microarray / Gene expression |
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| Research Abstract |
It is reported that cancer cells interacted with interstitial cells and regulated tumor progression. We investigated the interaction between interstitial cells and genitourinary tumor, and detected target molecules using microarray. HGF is one of the growth factor secreted by interstitial cells. In bladder cancer cell lines, J82, T24, HT1376, and 5637 cells, HGF stimulated tumor cell scatter and invasion. The gene profile of 5637 indicated that only MMP-3 expression was induced by HGF which was confirmed by microarray and RT-PCR. The expression level of MMP-3 in invasive bladder tumor was higher than that in superficial bladder tumor (p=0.0054). There was a correlation between MMP-3 expressions in bladder cancer tissue and HGF concentrations in serum. These results suggest that HGF acts as an invasion factor in the results of induction of MMP-3 expression in bladder cancer. The expression of MMP-3 in bladder cancer suggests an important role in bladder cancer progression.
Next, we added the conditioned media obtained from bladder cancer cell line, 5637 cells, into human fibroblast cell lines, TIG-1. TIG-1 cells cultured by conditioned media obtained from 5637 cells were stimulated invasive activity and secretion of HGF into media. In microarray assay, growth differentiation factor 5, connective tissue growth factor, and caveolin 1 were overexpressed in TIG-1 cells cultured with conditioned media obtained from bladder cancer, 5637 cells, suggesting that these molecules might play an important role in tumor invasion and become the target gene of bladder cancer for gene therapy.
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