The analysis of the mechanism of the construction of skin texture using fetal wound healing model.

• Project/Area Number: 15390541
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Keio University
• Principal Investigator: KISHI Kazuo Keio University, Department of Medicine, lecturer, 医学部, 講師 (40224919)
• Co-Investigator(Kenkyū-buntansha): NAKAJIMA Tatsuo Keio University, Department of Medicine, professor, 医学部, 教授 (40095633) ; KATSUBE Kennichi Tokyo medical and dental school, assistant professor, 医学部, 助教授 (20233760)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥11,000,000 (Direct Cost: ¥11,000,000); Fiscal Year 2004: ¥5,300,000 (Direct Cost: ¥5,300,000); Fiscal Year 2003: ¥5,700,000 (Direct Cost: ¥5,700,000)
• Keywords: fetal / wound healing / Genechip / sonic hedgehog / real time RTPCR / 皮膚 / 再生 / きめ / 皮溝 / 皮丘

Research Abstract

In murine fetuses, we found that wounds heal completely until embryonic day (E)13 including skin texture that do not regenerate after E14 wound healing. Based on these data, we tried to find out the mechanism how the skin texture regenerate in E13 wound healing. We compared the expression pattern of sonic hedgehog (shh), indian hedgehog, desert hedgehog, gli-1,-2,-3, smoothened, elastin, fibrillin, fiburin. Although shh did not expressed in whole stages of E13 wound healing, shh strongly expressed in E14 wound healing. Gli-1,-2,-3, smoothened did expressed in E13 wound healing. These results indicate that the overexpression of shh after E14 wound healing may disrupt the regeneration of skin texture.
Using Genechips, we found other molecules that expressed in E13 but not E14 wound healing. In these molecules, we could found 12 genes including Igals9, clu and so on. On the other hand, 9 genes that did overexpressed in E14but not E13 were found. These molecules have a possibility that they may regulate the pattern of skin texture.

Research Products

• [Journal Article] Transplanted mesenchymal stein cells are effective for skin regeneration in acute cutaneous wounds. (2004)
  • Author(s): Satoh H, Kishi K, et al.
  • Journal Title: Cell Transplant. 13(4) Pages: 405-412
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Transplanted mesenchymal stem cells are effective for skin regeneration in acute cutaneous wounds. (2004)
  • Author(s): Satoh H, Kishi K, Tanaka T, et al.
  • Journal Title: Cell Transplant. 13(4) Pages: 405-412
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] スキッドマウス背部での皮膚再生モデル
  • Author(s): 貴志和生ら
  • Journal Title: 形成外科 (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure.
  • Author(s): Kazuo Kishi, Kenichi Katsube, et al.
  • Journal Title: Cell Transplant (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The fetal dermal but not loose fascial mesenchyma cells possess regenerative activity of dermal structure.
  • Author(s): Kazuo Kishi, Kenichi Katsube, Hiroko Satoh, et al.
  • Journal Title: Cell Transplant (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The fetal dermal but not loose fascial mesenchymal cells possess regenerative activity of dermal structure
  • Author(s): Kazuo Kishi et al.
  • Journal Title: Cell transplantation (in press)
• [Book] 創傷治癒 (2005)
  • Author(s): 貴志和生
  • Total Pages: 209
  • Publisher: ブレーン出版
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 創傷治癒
  • Author(s): 貴志和生
  • Publisher: Scarless wound healing (in press)
• [Publications] 貴志和生: "Scarless Wound Healing"治療. 85・10. 79-83 (2003)