| Project/Area Number |
15405036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | HOKKAIDO UNIVERSITY (2005)
Obihiro University of Agriculture and Veterinary Medicine (2003-2004) |
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Principal Investigator |
SUGIMOTO Chihiro Hokkaido University, Research Center for Zoonosis Control, Professor, 人獣共通感染症リサーチセンター, 教授 (90231373)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISAKI Kozo Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (00292095)
XUAN Xuenan Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Professor, 原虫病研究センター, 教授 (10292096)
INOUE Noboru Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (10271751)
YOKOYAMA Naoaki Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Protozoan Diseases, Associate Professor, 原虫病研究センター, 助教授 (80301802)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 2005: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2003: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Theileria / Babesia / Trypanosoma / China / protozaoa / small ruminant / 住血微生物 |
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| Research Abstract |
Hemoprotozaon parasite closely related to Chinese Theileria species in Sheep was detected from ticks, Haemaphysalis japonicum infesting on Japanese serow and blood samples of serow by using PCR targeting small subunit ribosomal RNA gene of Chinese Theileria sp. from sheep. However, its infectivity to splenectomized sheep was not confirmed when blood from infected serow was injected. Therefore, transmission of Theileria parasites between domestic and wild ruminants was considered no to occur under natural condition.
Several methods based on loop-mediated DNA amplification (LAMP) have been developed for diagnoses of diseases caused by hemoprotozoan parasites, including equine Babesia/Trypanosoma, ovine Babesia/Theileria. Specificity and sensitivity of these tests had been demonstrated to be high as compared with the results obtained by PCR tests. Infection rate of Trypanosoma evansi among horses in Xinjian area exceeded 90 % as determined by LAMP. These LAMP methods will be useful for molecular epidemiological studies of hemoprotozoan parasites in China.
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