Respiratory rhythm generation and respiratory neuron networks
| Project/Area Number |
15500293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tokyo Metropolitan Organization for Medical Research |
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Principal Investigator |
EZURE Kazuhisa Tokyo Metropolitan Organization for Medical Research, Tokyo Metropolitan Institute for Neuroscience, Department Director, 東京都神経科学総合研究所, 参事研究員 (20132904)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Respiratory Rhythm / Botzinger Complex / Pre-Botzinger Complex / Inhibitory Network / グリシン / GABA / GLYT2 / リズム / 漸減型呼息性ニューロン / 共放出 |
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| Research Abstract |
The brainstem respiratory network, which involves a variety of respiratory neurons and constitutes the respiratory center, generates the automatic rhythmicity of respiration and the spatio-temporally organized contraction of respiration-related muscles. In vivo electrophysiological and neuroanatomical studies using largely cats and rats, have greatly increased our knowledge about the overall respiratory system and its network mechanisms. knowledge about the overall respiratory system and its network mechanisms. Currently, a limited region of the ventrolateral medulla, which spans the areas called the Botzinger complex and the pre-Botzinger complex, is the focus of our attention. In this region, a number of excitatory and inhibitory neurons with specific firing patterns and characteristic morphological features have been identified. These neurons form the networks that involve 1)inhibitory connections between inspiratory and expiratory neurons, 2)excitatory connections between inspiratory neurons, 3)inhibitory connections seem to be essential for the generation of respiratory rhythm, and some for the formation of patterned respiratory outputs. To proceed with the network analysis, we tried to determine the transmitter(s) of inhibitory inhibitory neurons of the Botzinger complex, by using single cell labeling and in situ hybridization of mRNA for glycine transporter 2(GLYT2). Both augmenting and decrementing expiratory neurons of the Botzinger complex had GLYT2 mRNA activity, suggesting that those neurons use glycine as their inhibitory transmitter. Furthermore, we found that other decrementing expiratory neurons that were located caudally in the ventral respiratory group had GLYT2mRNA. This suggested that these expiratory neurons are also inhibitory neurons and use glycine.
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Report
(3 results)
Research Products
(31 results)
