Studies on phytoextraction and phytomining of heavy metals

• Project/Area Number: 15510073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Mie University
• Principal Investigator: OBATA Hitoshi Mie University, Graduate School of Bioresources, Sustainable Resource Sciences, Professor, 大学院生物資源学研究科, 教授 (70024594)
• Co-Investigator(Kenkyū-buntansha): MIZUNO Takafumi Mie University, Graduate School of Bioresources, Sustainable Resource Sciences, Associate Professor, 大学院生物資源学研究科, 助教授 (50346003)
• Project Period (FY): 2003 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,600,000 (Direct Cost: ¥3,600,000); Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: phytoremediation / phytomining / hyperaccumulator / Ni / Mn / metal transporters / phytochelatin / Thlaspi japonicum / カドミウム / グンバイナズナ / コシアブラ / マンガン可溶化 / Thlaspi japonicum / TjZNT / ニッケル輸送能 / マンガン集積 / 緑化用ソバ / 鉛集積 / プロトン放出 / Thlasip japonicum / ニッケル輸送 / ZIP family transporter / Nramp family transporter / TjZNT1 / TjZNT2 / TjNRAMP1 / Histidine rich domain / ファイトレメディエーション / 重金属超集積性植物 / Thlaspi / フィトケラチン合成酵素 / カドミウム耐性 / 金属輸送

Research Abstract

The main purposes of this study are clarification of the role of metal-binding peptides, phytochelatin, and functional analysis of transporters in heavy metal transport and homeostasis using Ni hyperaccumulator Thlaspi japonicum and Mn hyperaccumulator Chengiopanax sciadophylloides, ordinary plants in Japan.
1. Ni/Zn accumulation ability of T. japonicum ecotypes from three different areas in Hokkaido was compared. T. japonicum plants were able to accumulate high Ni/Zn accumulation regardless ecotype and habitat.
2. The phytochelatin synthase gene was cloned from T. japonicum and designated as TjPCS. The yeast transformed with the TjPCSshowed an obvious Cd^<2+> tolerance. The same gene was cloned from a Cd hyperaccumulator plant Thlaspi caerulescens.
3. Homologous genes of ZIP and NRAMP metal transporters were cloned from T. japonicum and two ZIP transporter genes (TjZNT1, 2) and one NRAMP gene (TjNRAMP4) were cloned. The yeast transformed with TjZNT1, 2 showed a marked increase in Ni^<2+> tolerance. In contrast, the expression of TjNRAMP4 caused elevation of Ni^<2+> sensitivity and Ni^<2+> concentration.
4. C. sciadophylloides lowered the pH of the culture solution continuously and simultaneously solubilized MnO_2 in the medium Further studies on each gene expression in plants are needed to clarify the role of these genes in plants and to use the transformed plants for phytoremediation.

Research Products

• [Journal Article] Expression of Thlaspi caerulescens phytochelatin synthase gene and its role in Cd tolerance in yeast (2007)
  • Author(s): Mizuno, T., Sonoda, T., Sugiura, D., Obata, H.
  • Journal Title: Bull. Fac. Bioresources Mie Univ. (accepted)
  • NAID: 40015579822
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Continual pH lowering and manganese dioxide solubilization in the rhizosphere of the Mn-hyperaccumulator plant Chengiopanax sciadophylloides. (2006)
  • Author(s): Mizuno, T., Hirano, K., Hosono, A., Kato, S., Obata, H.
  • Journal Title: Soil Sci. Plant Nutr. 52 Pages: 726-733
  • NAID: 110006251082
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparison of Ni/Zn accumulation ability of Thlaspi japonicum from three different areas in Hokkaido. (2005)
  • Author(s): Mizuno, T., Obata, H., Horie, K., Nosaka, S., Mizuno, N.
  • Journal Title: Soil Sci. Plant Nutr. 51 Pages: 589-594
  • NAID: 110004704779
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant Thlaspi japonicum and their Ni^<2+>-transport abilities. (2005)
  • Author(s): Mizuno, T., Usui, K., Horie, K., Nosaka, S., Mizuno, N., Obata, H.
  • Journal Title: Plant Physiol. Biochem. 43 Pages: 793-801
  • NAID: 120000948971
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant Thlaspi japonicum and their Ni2+-transport abilities. (2005)
  • Author(s): Mizuno, T., Usui, K., Horie, K., Nosaka, S., Mizuno, N., Obata, H.
  • Journal Title: Plant Physiol. Biochem. 43 Pages: 793-801
  • NAID: 120000948971
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Decontamination of Chlorate in Longan Plantation Soils by Bio-Stimulation with Molasses Amendment. (2005)
  • Author(s): Sutigoolabud, P., Senoo, K., Ongprasert S., Mizuno, T., Mishima, T., Hisamatsu, M., Obata, H.
  • Journal Title: Soil Science and Plant Nutrition 51・4 Pages: 583-588
  • NAID: 110004704778
• [Journal Article] Comparison of Ni/Zn metal accumulation ability of Thlaspi japonicum from three different areas in Hokkaido. (2005)
  • Author(s): Mizuno, T., Obata, H., Horie, K., Nosaka, S., Mizuno, N
  • Journal Title: Soil Science and Plant Nutrition 51・4 Pages: 589-594
• [Journal Article] Cloning of three ZIP/Nramp transporter genes from a Ni hyperaccumulator plant Thlaspi japonicum and their Ni^<2+>-transport abilities. (2005)
  • Author(s): Mizuno T, Usui K, Horie K, Nosaka S, Mizuno N, Obata H.
  • Journal Title: Plant Physiology and Biochemistry 43・8 Pages: 793-801
  • NAID: 120000948971
• [Journal Article] 和歌山県産南高梅の梅干し加工工程におけるミネラルおよび有機酸含量の変化 (2005)
  • Author(s): 古市幸生, 水野隆文, 山下佳伸, 鈴木淳史, 小畑 仁, 梅宮善章
  • Journal Title: 日本食品科学工学会誌 52・10 Pages: 472-478
• [Journal Article] Cloning and characterization of phytochelatin synthase from a nickel hyperaccumulator Thlaspi japonicum and its expression in yeast. (2003)
  • Author(s): Mizuno, T., Sonoda, T., Horie, K., Senoo, K., Tanaka, A., Mizuno, N., Obata, H.
  • Journal Title: Soil Sci. Plant Nutr. 49 Pages: 285-290
  • NAID: 10012147911
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cloning and characterization of phytochelatin synthase from a nickel hyperaccumulator Thlasp* japonicum and its expression in yeast. (2003)
  • Author(s): Mizuno, T., Sonoda, T., Horie, K., Senoo, K., Tanaka, A., Mizuno, N., Obata, H.
  • Journal Title: Soil Sci. Plant Nutr. 49 Pages: 285-290
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of Thlaspi caerulescens phytochelatin synthase gene and its role in Cd tolerance in yeast
  • Author(s): Mizuno, T., Sonoda, T., Sugiura, D., Obata, H.
  • Journal Title: Bull. Fac. Bioresources Mie Univ. (accepted)
  • NAID: 40015579822
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of Thlaspi caerulescens phytochelatin synthase gene and its role in Cd tolerance in yeast
  • Author(s): Mizuno, T., Sonoda, T., Sugiura, D., Obata, H.
  • Journal Title: Bull. Fac. Bioresources Mie Univ. (accepted)
  • NAID: 40015579822
• [Book] Plant Nutrition for food security, human health and environmental protection. (2005)
  • Author(s): Mizuno T., Obata H.
  • Total Pages: 1207
  • Publisher: Tsinghua University Press