In vivo detection and identification of free radicals
| Project/Area Number |
15510179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
IWAHASHI Hideo Wakayama Medical University, Medical School, Professor, 医学部, 教授 (50145926)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | Free radicals / ESR / Ether radical / Lipid peroxidation / ferrous ions / microdialysis / flavone / HPLC-ESR-MS / Radicals / HPLC / MS / Linoleic acid / Active Oxygen / Lipid Peroxidation / Spin Trapping |
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| Research Abstract |
(1)7-Carboxyheptyl radical, pentyl radical, 7-carboxyldihydroxyheptyl radical, 1,5-dihydroxypentyl radical, 8-carboxy-1-hydroxyoctyl radical, 7-carboxy-1-hydroxyheptyl radical, 1-hydroxypentyl radical, and 1-hydroxyhexyl radical were detected and identified in the reaction mixture of oxidized linoleic acid with ferrous ions using HPLC-ESR-MS. (2)7-Carboxyheptyl radical is formed in the reaction mixtures of 13-hydroperoxy-9,11-octadecadienoic acid with several hemoproteins through a mechanism different from the one in the reaction of 13-hydroperoxy-9,11-octadecadienoic acid with ferrous ions. (3)Pentyl radicals were detected and identified in the reaction mixtures of 1,2-dilinoleoyl-sn-glycero-3-phosphochline (DLPC) with soybean lipoxygenase, oxidized DLPC with ferrous ions, and DLPC hydroperoxide with ferrous ions. (4)A flavone glucoside, luteolin-7-O-glucoside (luteolin-7-G) inhibited the formation of pentyl and 7-carboxyheptyl radicals in the reaction of 13-hydroperoxy-9,11-octadecadienoic acid (13-HPODE) with iron (II) ions. The inhibitory effects of luteolin-7-G occur partly through the chelation of iron ions. (5)The radicals detected in the reaction mixture of ferrous ions with the serums from rats exposed to diethyl ether were identified as 1-ethoxyethyl radicals. (6)Ethyl radicals were detected in the reaction mixture of rat brain homognate with ascorbate/EDTA/ions system. (7)Radicals were detected in rat brain after reperfusion using microdialysis.
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Report
(3 results)
Research Products
(11 results)
