Genomic analysis of Drosophila ananassae and its sibling species by means of parthenogenetic hybrid
| Project/Area Number |
15570002
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | University of Tsukuba |
|---|
Principal Investigator |
SAWAMURA Kyoichi University of Tsukuba, Graduate School of Life and Environmental Sciences, Assistant Professor, 大学院・生命環境科学研究科, 講師 (90247205)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OGUMA Yuzuru University of Tsukuba, Graduate School of Life and Environmental Sciences, Professor, 大学院・生命環境科学研究科, 教授 (90114074)
SATO Hajime Kyorin University, School of Medicine, Lecturer, 医学部, 講師 (70301299)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Drosophila / Genome / Interspecific hybrid / Parthenogenesis / Molecular marker / Chromosome inversion / Reproductive isolation / Speciation |
|---|
| Research Abstract |
Hybrids were made from the cross of parthenogenetic strains of Drosophila ananassae (a unisexual impaternate strain females) and D.pallidosa (a bisexual bridge strain males). As the former has a visible marker yellow on XL (the left arm of the X chromosome ; hereafter chromosome arms are abbreviated similarly (L, the left arm ; R, the right arm)), hybridism is confirmed by the yellow^+ phenotype of the females. The F_1 females produce offspring parthenogenetically and 266 lines were established from individual F_2 females. The lines have various genotypes with interspecific mosaic genomes which are made homozygous (i.e., iso-genic). We investigated the genomic structure (the species origin of every chromosome region) of each mosaic genome lines. Chromosome inversions In(3R)A of D.ananassae and In(XL)A, In(2L)CD+B and In (3R)B of D. pallidosa were utilized for cytological markers. 6 PCR-RFLP markers whose cytological locations have been determined (w (XR), Om (2D)(2mid), eyg (2R), cn (3L), Amyrel (3mid) and RfaBp (4)) plus 2 RAPD markers completely linked to the above-mentioned inversions (OPA15-950 (2L) and OPA15-1200 (3R)) were also utilized. The genomic structure of all the lines has been determined. The statistical test indicated quasi-linkage between 2L and 3R. This might be caused by either (1)low fitness (fertility or viability) of some genotypes or (2)simultaneous distribution of the chromosomes of the same species origin in meiosis. We are going to use the interspecific mosaic genome lines established here for the mapping of genes determining species-specific characters (including sexual behavior).
|
Report
(3 results)
Research Products
(17 results)
