Analyses of responsible genes of morphological mutants in the Japanese morning glory.
| Project/Area Number |
15570007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NITASAKA Eiji Kyushu University, Faculty of Science, Research Associate, 大学院・理学研究院, 助手 (60222189)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Ipomoea nil / FEATHERED / morphogenesis / transposable elements / KAN1 / Japanese morning glory / petaloid |
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| Research Abstract |
The semi-dominant feathered (fe) mutants in the Japanese morning glory have strongly crumpled or rolled leaves, and flowers with deeply split or tube-like petals folding back at their tips. Histological analysis showed that the number of stomata in abaxial side decreased in fe mutants. These mutant Phenotypes suggest that FE is involved in establishment of organ polarity. We isolated a rare fe revertant caused by excision of a transposable element inserted in FE gene. Tpn1 family which belong to En/Spn related transposable elements is known to induce most mutations in the Japanese morning glory. FE gene was isolated using Simplified Transposon Display(STD) method which allows visualization and isolation of Tpn transposon-flanking sequences from high copy-number lines. FE gene encodes a protein in the GARP family of putative transcription factors, and has high homology with KANADI1(KAN1) gene of Arabidopsis. We suppose that FE expresses on abaxial side of lateral organs, and specify the
… More
abaxial identity as KAN. Gentic and molecular analyses showed that the strong fe phenotype is caused by an additional mutation occurred in fe background.
The prtaloid(pt) mutants have petaloid stamens and short sepaloid carpel. This phenotype seems to be caused by the mutation of a C-function floral homeotic gene. Mutations of duplicated(dp) cause a substitution of reproductive organs to perianth organs (petals and sepals). An En/Spm-related transposable element, Tpn-botan, was inserted in the second intron of a C-funtion MADS-box gene DP and the subsequent excision event led to a deletion of a substantial part of the original Tpn element and DP genome (dp-1 allele). We assumed that pt is a week DP allele and examined the genomic structure of the second intron of DP gene in pt mutants. A full length Tpn 109 was inserted in the same place of dp-1 allele in the pt mutant. It is known that pt phenotypes become weaker during shoot growth, but Tpn 109 insertion was still obeserved in weak phenotype flower. Therefore, pt phenotype is caused by reduction of DP gene transcripts by low splicing efficiency or split cis-regulatory elements. To examine the deletion mechanism mediated by Tpn transposon, I analyzed genomic structures of other two dp alleles (dp-2, dp-3). dp-2 and dp-3 alleles have two and three rearranged Tpn 109 elements, respectively, and genomic region between two Tpn 109 elements was deleted. Less
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Japanese morning glory dusky mutants displaying reddish-brown or purplish-gray flowers are deficient in a novel glycosylation enzyme for anthocyanin biosynthesis, UDP-glucose : anthocyanidin 3-O-glucoside-2"-O-glucosyltransferase, due to 4-bp insertions in the gene.2005
Author(s)
Morita Y, Hoshino A, Kikuchi Y, Okuhara H, Ono E, Tanaka Y, Fukui Y, Saito N, Nitasaka E, Noguchi H, Iida S.
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Journal Title
Plant J. 42
Pages: 353-363
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] A dwarf mutant strain of Pharbitis nil, Uzukobito (kobito), has defective brassinosteroid biosynthesis.2003
Author(s)
Suzuki Y, Saso K, Fujioka S, Yoshida S, Nitasaka E, Nagata S, Nagasawa H, Takatsuto S, Yamaguchi I.
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Journal Title
Plant J. 36
Pages: 401-410
Description
「研究成果報告書概要(欧文)」より
Related Report
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