Molecular mechanisms of the adult brain-specific genomic imprinting of mouse Mzrr1 gene.
| Project/Area Number |
15570008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Saga University (2004)
佐賀医科大学 (2003) |
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Principal Investigator |
JOH Keiichiro Saga University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (90124809)
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| Co-Investigator(Kenkyū-buntansha) |
MUKAI Tsunehiro Saga University, Faculty of Medicine, Professor, 医学部, 教授 (40108741)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Genomic Imprinting / Transcriptional interference / Murr1 gene / U2af1-rs1 gene / Murrl遺伝子 |
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| Research Abstract |
The mouse Murr1 gene contains an imprinted gene, U2af1-rs1, in its first intron. U2af1-rs1 shows paternal allele-specific expression and is transcribed in the opposite direction to Murr1 gene. In contrast to the previous report of biallelic expression of Murr1 in neonatal mice, we have found that the maternal allele is expressed predominantly in adult brain and also preferentially in other adult tissues. This maternal-predominant expression is not observed in embryonic and neonatal brains. In situ hybridization experiments on adult brain indicated that Murr1 gene was maternally expressed in neuronal cells in all regions of the brain. We have analyzed the developmental change in expression levels of both Murr1 and U2af1-rs1 in brain and liver, and propose that the maternal predominant expression of Murr1 results from transcriptional interference of the gene by U2af1-rs1,through the Murr1 promoter region. The Murr1/U2af1-rs1 locus described here might represent a prototype arrangement of imprinted genes before they evolved to become located in larger imprinted chromosomal domains.
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Report
(3 results)
Research Products
(4 results)
