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Studies on Molecular Basis for Reactive Nitrogen Metabolism in Plants

Research Project

Project/Area Number 15570038
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

SAKAMOTO Atsushi  Hiroshima University, Graduate School of Science, Associate Professor, 大学院・理学研究科, 助教授 (60270477)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsReactive nitrogen species / 2-Cys peroxiredoxin / Non-symbiotic hemoglobin / S-nitrosothiol / GSNO reductase / Peroxynitrite / Nitrite / Arabidopsis / 一酸化窒素(NO) / NOシグナリング / S-ニトロソグルタチオン還元酵素 / タンパク質S-ニトロソ化 / ストレス / 窒素代謝 / ニトロソチオール / ヘモグロビン / パーオキシナイトライト
Research Abstract

(1)Arabidopsis 2-Cys Peroxiredoxin as Metabolic Enzyme for Reactive Nitrogen Species
Purified recombinant proteins of a thiol-dependent peroxidase called 2-Cys peroxiredoxin (2CPRX) from Arabidopsis were shown to metabolically scavenge peroxynitrite, a reactive nitrogen species (RNS) known as a potent oxidizing and nitrating agent. The Arabidopsis 2CPRX cDNA was functionally able to complement the hypersensitivity of a yeast mutant to nitrite-derived RNS. These results demonstrated a new role of plant 2CPRX as a critical determinant of the resistance to RNS, and support the existence of a plant enzymatic basis for RNS metabolism.
(2)Functional relevance of Arabidopsis Non-Symbiotic Hemoglobin to Reactive Nitrogen Species
Plants ubiquitously contain non-symbiotic hemoglobins whose physiological role remains obscure. Purified recombinant proteins of an Arabidopsis non-symbiotic hemoglobin (AtGLB1) were shown to possess peroxidase-like activity that can oxidize nitrite as electron donor to f … More orm nitrogen dioxide radicals. AtGLB1 mRNA significantly accumulated in Arabidopsis seedlings that had been exposed to nitrite. These results support the physiological relevance of the function of AtGLB1 to nitrite and nitrite-derived RNS, and suggest a new possible route for RNS production in plants, apart from nitric-oxide (NO) biogenesis.
(3)Plant-Physiological Impact of Molecular-Genetic Alteration in S-Nitrosothiol Metabolism
S-Nitrosothiols (RSNO), a major form of RNS, are NO-adducts of thiol compounds such as S-nitrosoglutathione (GSNO) that is a representative RSNO with biological relevance. Overexpression of Arabidopsis GSNO reductase (GSNOR), the only known plant enzyme possibly involved in RSNO metabolism, led to a significant RSNO reduction in transgenic Arabidopsis. In contrast, knocking out the GSNOR gene resulted in RSNO over-accumulation. Metabolic studies of NO_2 assimilation in transgenic Arabidopsis overexpressing GSNOR suggested the existence of a crosstalk between RNS metabolism and the primary nitrogen assimilation. These results strongly indicate GSNOR as a key enzyme in RSNO metabolism and its potential importance in plant nitrogen metabolism. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (56 results)

All 2005 2004 2003 Other

All Journal Article (44 results) Book (3 results) Publications (9 results)

  • [Journal Article] Novel metabolism of nitrogen in plants2005

    • Author(s)
      Morikawa et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 265-271

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidopsis thaliana2005

    • Author(s)
      Kurumata et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 272-278

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nocturnal uptake and assimilation of nitrogen dioxide by C3 and CAM plants2005

    • Author(s)
      Takahashi et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 279-284

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] TJ1 is an orientation-independent transformation enhancer sequence2005

    • Author(s)
      Hokazono et al.
    • Journal Title

      Plant Biotechnology 22

      Pages: 137-140

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phytoremediators from abandoned rice field2005

    • Author(s)
      Takahashi et al.
    • Journal Title

      Plant Biotechnology 22

      Pages: 167-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Atmospheric nitrogen dioxide gas is a plant vitalization signal to improve plant size and constituents2005

    • Author(s)
      Morikawa et al.
    • Journal Title

      New Phytologist (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel metabolism of nitrogen in plants.2005

    • Author(s)
      Morikawa et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 265-271

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tolerance to, and uptake and degradation of 2,4,6-trinitrotoluene (TNT) are enhanced by the expression of a bacterial nitroreductase gene in Arabidopsis thaliana.2005

    • Author(s)
      Kurumata et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 272-278

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Nocturnal uptake and assimilation of nitrogen dioxide by C3 and CAM plants.2005

    • Author(s)
      Takahashi et al.
    • Journal Title

      Zeitschrift fur Naturforschung 60c

      Pages: 279-284

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] TJ1 is an orientation-independent transformation enhancer sequence.2005

    • Author(s)
      Hokazono et al.
    • Journal Title

      Plant Biotechnology 22

      Pages: 137-140

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Phytoremediators from abandoned rice field.2005

    • Author(s)
      Takahashi et al.
    • Journal Title

      Plant Biotechnology 22

      Pages: 167-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Atomospheric nitrogen dioxide gas is a plant vitalization signal to improve plant size and constituents.2005

    • Author(s)
      Morikawa et al.
    • Journal Title

      New Phytologist (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Higher plants and metabolism of oxides of nitrogen.2005

    • Author(s)
      Morikawa et al.
    • Journal Title

      Nitrogen Nutrition and Sustainable Plant Productivity (R.P.Sigh, N.Shankar, P.K.Jaiwal, eds.) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Uptake, assimilation and novel metabolism of nitrogen dioxide in plants.2005

    • Author(s)
      Takahashi et al.
    • Journal Title

      Methods in Biotechnology : Phytoremediation Methods and Review (N.Willey, ed.) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conversion of the nitrate nitrogen and nitrogen dioxide to nitrous oxides in plants2004

    • Author(s)
      Hakata et al.
    • Journal Title

      Acta Biotechnologica 23

      Pages: 249-257

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic modification of fatty acid unsaturation of phosphatidylglycerol in chloroplasts alters the sensitivity of tobacco plants to cold stress2004

    • Author(s)
      Sakamoto et al.
    • Journal Title

      Plant, Cell and Environment 27

      Pages: 99-105

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism2004

    • Author(s)
      Morikawa et al.
    • Journal Title

      Planta 217

      Pages: 14-22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR2004

    • Author(s)
      Kato et al.
    • Journal Title

      Journal of Experimental Botany 55

      Pages: 1761-1763

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage2004

    • Author(s)
      Park et al.
    • Journal Title

      The Plant Journal 40

      Pages: 474-487

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 活性窒素代謝 -植物窒素代謝の新しい課題-2004

    • Author(s)
      坂本 敦, 他
    • Journal Title

      日本農芸化学会誌 78

      Pages: 958-961

    • NAID

      10013654756

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration.2004

    • Author(s)
      Sakamoto et al.
    • Journal Title

      FEBS Letters 572

      Pages: 27-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic modification of fatty acid unsaturation of phosphatidylglycerol in chloroplasts alters the sensitivity of tobacco plants to cold stress.2004

    • Author(s)
      Sakamoto et al.
    • Journal Title

      Plant, Cell and Environment 27

      Pages: 99-105

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism.2004

    • Author(s)
      Morikawa et al.
    • Journal Title

      Planta 219

      Pages: 14-22

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR.2004

    • Author(s)
      Kato et al.
    • Journal Title

      Journal of Experimental Botany 55

      Pages: 1761-1763

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage.2004

    • Author(s)
      Park et al.
    • Journal Title

      The Plant Journal 40

      Pages: 474-487

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Reactive nitrogen metabolism, a new frontier in plant nitrogen metabolism (in Japanese).2004

    • Author(s)
      Sakamoto et al.
    • Journal Title

      Nippon Nogeikagakukai Kaishi 78

      Pages: 958-961

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration2004

    • Author(s)
      A.Sakamoto et al.
    • Journal Title

      FEBS Letters 572・8

      Pages: 27-32

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic modification of fatty acid unsaturation of phosphatidylglycerol in chloroplasts alters the sensitivity of tobacco plants to cold stress2004

    • Author(s)
      A.Sakamoto et al.
    • Journal Title

      Plant, Cell and Environment 27・1

      Pages: 99-105

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Genetic engineering of glycinebetaine synthesis in tomato protects seeds, plants, and flowers from chilling damage2004

    • Author(s)
      E.-J.Park et al.
    • Journal Title

      The Plant Journal 40・11

      Pages: 474-487

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Differential expression of the nitrite reductase gene family in tobacco as revealed by quantitative competitive RT-PCR2004

    • Author(s)
      C.Kato et al.
    • Journal Title

      Journal of Experimental Botany 55・8

      Pages: 1761-1763

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism2004

    • Author(s)
      H.Morikawa et al.
    • Journal Title

      Planta 219・5

      Pages: 14-22

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 活性窒素代謝 -植物窒素代謝の新しい課題-2004

    • Author(s)
      坂本 敦, 他
    • Journal Title

      日本農芸化学会誌 78・10

      Pages: 958-961

    • NAID

      10013654756

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Functional complementation in yeast reveals a protective role of chloroplast 2-Cys peroxiredoxin against reactive nitrogen species2003

    • Author(s)
      Sakamoto et al.
    • Journal Title

      The Plant Journal 33

      Pages: 841-851

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Screening and genetic manipulation of plants for decontamination of pollutants from the environments2003

    • Author(s)
      Morikawa et al.
    • Journal Title

      Acta Biotechnologica 22

      Pages: 9-15

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Genetic engineering of cold-tolerant tomato via glycinebetaine biosynthesis.2003

    • Author(s)
      Park et al.
    • Journal Title

      Cryobiology and Cryotechnology 49

      Pages: 77-85

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of regeneration and transformation systems for Raphiolepis umbellata L. plants using particle bombardment2003

    • Author(s)
      Erkin et al.
    • Journal Title

      Plant Biotechnology 20

      Pages: 145-152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 植物細胞の膜輸送系とファイトレメディエーション2003

    • Author(s)
      坂本 敦, 他
    • Journal Title

      植物細胞工学シリーズ18「植物の膜輸送システム-ポンプ・トランスポーター・チャネル研究の新展開」

      Pages: 146-150

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 窒素代謝とNOx代謝2003

    • Author(s)
      森川 弘道, 他
    • Journal Title

      蛋白質 核酸 酵素・2003年11月号増刊「植物の代謝コミュニケーション -植物分子生理学の新展開」 48

      Pages: 2130-2137

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Three distinct Arabidopsis hemoglobins exhibit peroxidase-like activity and differentially mediate nitrite-dependent protein nitration2003

    • Author(s)
      Sakamoto et al.
    • Journal Title

      FEBS Letters 572

      Pages: 27-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional complementation in yeast reveals a protective role of chloroplast 2-Cys peroxiredoxin against reactive nitrogen species.2003

    • Author(s)
      Sakamoto et al.
    • Journal Title

      The Plant Journal 33

      Pages: 841-851

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Conversion of the nitrate nitrogen and nitrogen dioxide to nitrous oxides in plants.2003

    • Author(s)
      Hakata et al.
    • Journal Title

      Acta Biotechnologica 23

      Pages: 249-257

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Screening and genetic manipulation of plants for decontamination of pollutants from the environments.2003

    • Author(s)
      Morikawa et al.
    • Journal Title

      Biotechnology Advances 22

      Pages: 9-15

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Development of regeneration and transformation systems for Raphiolepis umbellata L.plants using particle bombardment.2003

    • Author(s)
      Erkin et al.
    • Journal Title

      Plant Biotechnology 20

      Pages: 145-152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A novel aspect of nitrogen metabolism revealed by the study on the metabolism of nitrogen oxides in plants (in Japanese).2003

    • Author(s)
      Morikawa et al.
    • Journal Title

      Tanpakushitsu Kakusan Koso 48

      Pages: 2130-2137

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Nitrogen Nutrition and Sustainable Plant Productivity2005

    • Author(s)
      Morikawa et al.(分担執筆)
    • Publisher
      Studium Press LLC(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] Methods in Biotechnology : Phytoremediation Methods and Review2005

    • Author(s)
      Takahashi et al.(分担執筆)
    • Publisher
      Humana Press(印刷中)
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Book] 光合成事典(日本光合成学会編集)2003

    • Author(s)
      坂本 敦(分担執筆)
    • Total Pages
      410
    • Publisher
      学会出版センター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Sakamoto et al.: "Functional complementation in yeast reveals a protective role of chloroplast 2-Cys peroxiredoxin against reactive nitrogen species"The Plant Journal. 33. 841-851 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Sakamoto et al.: "Genetic modification of fatty acid unsaturation of phosphatidylglycerol chloroplasts alters the sensitivity of tobacco plants to cold stress"Plant, Cell and Environment. 27. 99-105 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Hakata et al.: "Conversion of the nitrate nitrogen and nitrogen dioxide to nitrous oxides in plants"Acta Biotechnologica. 23. 249-257 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Erkin et al.: "Development of regeneration and transformation systems for Raphiolepis umbellata L.plants using particle bombardment"Plant Biotechnology. 20. 145-152 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Morikawa et al.: "Screening and genetic manipulation of plants for decontamination of pollutants from the environments (review)"Biotechnology Advances. 22. 9-15 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Morikawa et al.: "Formation of unidentified nitrogen in plants : an implication for a novel nitrogen metabolism"Planta. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 坂本 敦, 他: "植物細胞の膜輸送系とファイトレメディエーション"細胞工学別冊・植物細胞工学シリーズ18「植物の膜輸送システム-ポンプ・トランスポーター・チャネル研究の新展開」. 146-150 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 森川 弘道, 他: "窒素代謝とNOx代謝 (A novel aspect of nitrogen metabolism revealed by the study on the metabolism of nitrogen oxides in plants)"蛋白質 核酸 酵素・2003年11月号増刊 「植物の代謝コミュニケーション-植物分子生理学の新展開」. 48. 2130-2137 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 坂本 敦(分担執筆): "光合成事典(日本光合成学会編集)"学会出版センター. 410 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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