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Contribution of the Detoxication of Reactive Aldehydes to Protection against Environmental Stress in Plants

Research Project

Project/Area Number15570039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物生理・分子
Research InstitutionYamaguchi University

Principal Investigator

MANO Jun'ichi  Yamaguchi University, Science Research Center, Associate professor, 総合科学実験センター, 助教授 (50243100)

Co-Investigator(Kenkyū-buntansha) MATSUI Kenji  Yamaguchi University, Faculty of Agriculture, Professor, 農学部, 教授 (90199729)
Project Period (FY) 2003 – 2005
Project Status Completed(Fiscal Year 2005)
Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 2005 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 2004 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 2003 : ¥1,300,000 (Direct Cost : ¥1,300,000)
Keywords2-alkenal reductase / environmental stress / lipid peroxide / aldehyde / phototolerance / chloroplast / acrolein / Calvin cycle / オゾン耐性 / α,β-不飽和アルデヒド / alkenal reductase / At5g167970 / 抗酸化防御 / 活性アルデヒド / 酸化還元酵素
Research Abstract

Antioxidative defense in cells is of critical importance in the plant protection against environmental stress.
We have recently identified in Arabidopsis thaliana a novel enzyme 2-alkenal reductase (AER) that catalyzes the reduction of the α,β-unsaturated bond of 2-alkenals, potent cytotoxins produced from lipid peroxides. In this study, we aimed at verifying a possible involvement of the lipid peroxide-derived 2-alkenals (reactive aldehydes) in the environmental stress-induced damages of plants. When A.thaliana leaves were oxidatively stressed, production of crotonaldehyde and acrolein was enhanced. Unlike the case for oxidatively stressed animal cells, malondialdehyde was not a major product. Thus, the metabolism of lipid peroxides in plants differs from that in animals. Acrolein and crotonaldehyde inhibited CO_2-fixation in isolated chloroplasts strongly, but did not affect the photosynthetic electron transport activity. Thus the reactive aldehydes specifically inhibit Calvin cycle activity, causing photoinhibition. We constructed three transgenic tobacco lines that over produced the A.thaliana AER.
The transgenic plants exhibited higher tolerance to methyl viologen and to intense light, indicating that AER protected leaf cells from oxidative stress. These results altogether indicate the involvement of the reactive aldehydes in the photooxidative injury and the significant contribution of AER to the antioxidative defense in leaves.

Report

(4results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report

Research Products

(19results)

All 2006 2005 2004 Other

All Journal Article Publications

  • [Journal Article] Seed deterioration due to high humidity at higgh temperature is suppressed by extremely low frequency magnetic field.2006

    • Author(s)
      Mano, J. 他 4名
    • Journal Title

      Seed Science and Technology 34

      Pages : 189-192

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Components of C6-aldehyde-induced resistance in Arabidopsis thaliana against a necrotrophic fungal pathogen, Botrytis cinerea.2006

    • Author(s)
      Kishimoto, K., Matsui, K.他 2名
    • Journal Title

      Plant Science 170

      Pages : 715-723

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seed deterioration due to high humidity and high temperature is suppressed by extremely low frequency magnetic fields.2006

    • Author(s)
      Mano, J., Nakahara, T., Torii, Y., Hirose, H.Miyakoshi, J., Takimoto, K.
    • Journal Title

      Seed Sci.Technol. 34

      Pages : 189-192

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Identification of aldehydes produced in leaves under oxidative stress.2006

    • Author(s)
      Mano, J., Khorobrykh, S., Okamitsu, K.
    • Journal Title

      Plant Cell Physiol. 47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Reactive aldehydes derived from lipid peroxides inhibit the Calvin cycle.2006

    • Author(s)
      Miyatake, F., Hiraoka, E., Mano, J.
    • Journal Title

      Plant Cell Physiol. 47

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Seed deterioration due to high humidity at higgh temperature is suppressed by extremely low frequency magnetic field.2006

    • Author(s)
      Mano, J.他4名
    • Journal Title

      Seed Science and Technology 34

      Pages : 189-192

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Components of C6-aldehyde-induced resistance in Arabidopsis thaliana against a necrotrophic fungal pathogen, Botrytis cinerea.2006

    • Author(s)
      Kishimoto, K., Matsui, K.他2名
    • Journal Title

      Plant Science 170

      Pages : 715-723

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls.2005

    • Author(s)
      Mano, J. 他 9名
    • Journal Title

      Plant Physiology 139

      Pages : 1773-1783

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls.2005

    • Author(s)
      Mano, J., Belles-Boix, E., Babiychuk, E., Inze, D., Torii, Y., Hiraoka, E., Takimoto, K., Slooten, L., Asada, K.Kushnir S.
    • Journal Title

      Plant Physiol. 139

      Pages : 1773-1783

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Volatile C6-aldehydes and allo-ocimene activate defense genes and induce resisitance against Botrytis cinerea in Arabidopsis thaliana.2005

    • Author(s)
      Kishimoto, K., Matsui, K., Ozawa, R.Takabayashi, J.
    • Journal Title

      Plant Cell Physiol. 139

      Pages : 1773-1783

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Protection against photooxidative injury of tobacco leaves by 2-alkenal reductase. Detoxication of lipid peroxide-derived reactive carbonyls.2005

    • Author(s)
      Mano, J.他9名
    • Journal Title

      Plant Physiology 139

      Pages : 1773-1783

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Seed deterioration due to high humidity at high temperature is suppressed by extremely low frequency magnetic field.2005

    • Author(s)
      Mano, J., Nakahara, T., 他4名
    • Journal Title

      Seed Science and Technology 33(3)(印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ascorbate in thylakoid lumen functions as an alternative electron donor to photosystem II and photosystem I.2004

    • Author(s)
      Mano, J. 他 2名
    • Journal Title

      Archives of Biochemistry and Biophysics 429

      Pages : 71-80

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Ascorbate in thylakoid lumen functions as an alternative electron donor to photosystem II and photosystemI.2004

    • Author(s)
      Mano, J., Hideg, E., Asada, K.
    • Journal Title

      Arch.Biochem.Biophys. 429

      Pages : 71-80

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Overexpression of 2-alkenal α,β-hydrogenase improved tolerance against photooxidative stress in tobacco.2004

    • Author(s)
      Mano, J.
    • Journal Title

      Plant and Cell Physiology 45(S)

    • Related Report
      2004 Annual Research Report
  • [Publications] Koeduka, T., Stumpe, M., Matsui, K., 他2名: "Kinetics of barley FA hydroperoxide lyase are modulated by salts and detergents"Lipids. 38. 1167-1172 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Matsui, K., Sasahara, S., Akakabe, Y., Kajiwara, T.: "Linoleic acid10-hydroperoxide as an intermediate during formation of 1-octen-3-ol from linoleic acid in Lentinus decadetes"Biosci.Biotechnol.Biochem.. 67. 2280-2282 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 松井健二, 有村源一郎: "情報科学物質として機能する植物揮発性成分の合成と制御"蛋白質核酸酵素. 48. 1793-1800 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kandzia, R., Stumpe, M., Berndt, E., Szalata, M., Matsui, K., Feussner, I.: "On the specificity of lipid hydroperoxide fragmentation by fatty acid hydroperoxide lyase from Arabidopsis thaliana"J.Plant Physiol.. 160. 803-809 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2003-04-01   Modified : 2016-04-21  

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