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Identification of unknown components in insect prophenoloxidase activating system.

Research Project

Project/Area Number15570059
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OCHIAI Masanori  Hokkaido Univ., Inst.of Low Temp.Sci., Inst., 低温科学研究所, 助手 (10241382)

Project Period (FY) 2003 – 2004
Project Status Completed(Fiscal Year 2004)
Budget Amount *help
¥3,200,000 (Direct Cost : ¥3,200,000)
Fiscal Year 2004 : ¥1,200,000 (Direct Cost : ¥1,200,000)
Fiscal Year 2003 : ¥2,000,000 (Direct Cost : ¥2,000,000)
Keywordsinsect / biologocal defense / innate immunity / nonself recognition / protease / prophenoloxidase activating system
Research Abstract

The prophenoloxidase (proPO) cascade of insect hemolymph is important for melanization around microbes invading the organisms and for recognition of foreignness. The cascade is triggered by microbial cell wall components such as β-1,3-glucan from fungi and peptidoglycan from bacteria. Although the interaction of peptidoglycan recognition protein (PGRP-S1) and peptidoglycan is thought to initiate a chain of enzymatic reactions in the peptidoglycan-mediated pathway of the cascade, the activating mechanism of the pathway has not been worked out yet. In this research the followings were made clear.
1.I demonstrated that Gram-negative bacteria-binding protein (GNBP) is an essential component of the peptidoglycan-mediated pathway in the proPO cascade of the silkworm, Bombyx mori and this protein does not directly bind to the peptidoglycan but bind to a complex of the peptidoglycan and PGRP-S1, forming a ternary complex. These results indicate that GNBP works together with PGRP-S1 for the recognition of bacterial peptidoglycan in insect innate immunity.
2.I purified a new essential component, Factor H, of the cascade from the silkworm plasma. The component could interacted with the ternary complex of peptidoglycan, PGRP-S1 and GNBP, and activates by itself, followed by the activation of a protease zymogen, Factor S. From analysis of internal sequences of Factor H, this protein was revealed to contain few sushi domains and a serine protease domain, suggesting that it is a novel zymogen of serine protease.
3.The effects of addition of polysaccharides and Ca^<2+>-regulatory protein to protease activity were systematically investigated for stabilization of Factor H. β-Cyclodextrin-sulfate and calmodulin-binding protein inhibited thrombin activity as a noncompetitive inhibition, suggesting that there are a possibility of suppressing spontaneous activation of Fatcor H.

Report

(3results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

Research Products

(16results)

All 2006 2005 2004 2003 Other

All Journal Article Publications

  • [Journal Article] A Spatzle-Processing Enzyme Required for Toll Signaling Activation in Drosophila Innate Immunity2006

    • Author(s)
      Jiang IH. et al.
    • Journal Title

      Dev. Cell 10

      Pages : 45-55

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Spatzle-Processing Enzyme Required for Toll Signaling Activation in Drosophila Innate Immunity2006

    • Author(s)
      Jiang, IH.et al.
    • Journal Title

      Dev.Cell 10

      Pages : 45-55

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of DNA structure on the formation of collagen-DNA complex2005

    • Author(s)
      Kaya M.et al.
    • Journal Title

      Int. J. Biol. Macromol. 35

      Pages : 39-46

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of DNA structure on the formation of collagen-DNA complex2005

    • Author(s)
      Kaya, M.et al.
    • Journal Title

      Int.J.Biol.Macromol. 35

      Pages : 39-46

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of DNA structure on the formation of collagen-DNA complex2005

    • Author(s)
      Kaya M.et al.
    • Journal Title

      Int.J.Biol.Macromol. (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of Mono-, Oligo- and Polysaccharides on Fibrin Gelation2004

    • Author(s)
      Masuda Y. et al.
    • Journal Title

      Trans. MRS-J. 29

      Pages : 3331-3335

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of Ca^<2+>^Dependent Calmodulin-binding Proteins in Rat Spermatogenic Cells as Complexes of the Heat-Shock Proteins2004

    • Author(s)
      Moriya M. et al.
    • Journal Title

      Mol. Reprod. Dev. 69

      Pages : 316-324

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of Ca^<2+>-Dependent calmodulin-binding Proteins in Rat Spermatogenic Cells as Complexes of the Heat-Shock Proteins2004

    • Author(s)
      Moriya, M.et al.
    • Journal Title

      Mol Reprod Dev. 69

      Pages : 316-324

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Mono-,Oligo- and Polysaccharides on Fibrin Gelation2004

    • Author(s)
      Masuda, Y.et al.
    • Journal Title

      Trans.MRS-J. 29

      Pages : 3331-3335

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of Mono-, Oligo- and Polysaccharides on Fibrin Gelation.2004

    • Author(s)
      Masuda Y. et al.
    • Journal Title

      Trans.MRS-J. 29

      Pages : 3331-3335

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Identification of Ca^<2+>-Dependent Calmodulin-binding Proteins in Rat Spermatogenic Cells as Complexes of the Heat-Shock Proteins2004

    • Author(s)
      Moriya M. et al.
    • Journal Title

      Mol Reprod Dev. 69

      Pages : 316-324

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effect of saccharides on the fibrinogen-Fibrin conversion by thrombin2003

    • Author(s)
      Kogire H. et al.
    • Journal Title

      Trans. MRS-J. 28

      Pages : 949-952

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of DNA structure on collagen fibrillogenesis2003

    • Author(s)
      Kaya M.et al.
    • Journal Title

      Peptide Sci. 2002

      Pages : 379-382

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of saccharides on the fibrinogen-Fibrin conversion by thrombin2003

    • Author(s)
      Kogure, H.et al.
    • Journal Title

      Trans.MRS-J. 28

      Pages : 949-952

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effect of DNA structure on collagen fibrillogenesis2003

    • Author(s)
      Kaya, M.et al.
    • Journal Title

      Peptide Sci. 2002

      Pages : 379-382

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kogure, H.: "Effect of saccharides on the fibrinogen-fibrin conversion by thrombin"Trans.MRS-J.. 28. 949-952 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2003-04-01   Modified : 2016-04-21  

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