Characterization of ubiquitin-like polypeptide acceptor protein, a novel pro-apoptotic member of the Bcl-2 family
| Project/Area Number |
15570118
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Shimane University (2004)
Shimane Medical University (2003) |
|---|
Principal Investigator |
NAKAMURA Morihiko Shimane University, Collaboration Center, Associate Professor, 産学連携センター, 助教授 (20155865)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TANIGAWA Yoshinori Shimane University, Faculty of Medicine, Professor, 医学部, 教授 (60084860)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | Ubiquitin / Apoptosis / Bcl-G / Suppressor / MALDI / Bcl |
|---|
| Research Abstract |
Monoclonal nonspecific suppressor factor(MNSF) is a cytokine with antigen nonspecific suppressive activity. MNSFβ (a subunit of MNSF) is a 14.5-kDa fusion protein consisting of a protein with 36% identity with ubiquitin and ribosomal protein S30. The ubiquitin-like segment (Ubi-L) may be cleaved from MNSFβ in the cytosol. Recently, we have observed that Ubi-L covalently binds to intracellular proteins in mitogen-activated murine T helper type 2 clone, D.10 cells. In this study, we purified a 33.5-kDa Ubi-L adduct from D.10 cell lysates by sequential chromatography on DEAE, anti-Ubi-L antibody conjugated Sepharose, and hydroxylapatite. MALDI-TOF-Mass fingerprinting revealed that this Ubi-L adduct consists of 8.5-kDa Ubi-L and Bcl-2-like protein, murine orthologue of previously cloned human BCL-G gene product with pro-apoptotic function. Murine Bcl-G mRNA was highly expressed in testis and significantly in spleen. In addition, the level of Bcl-G mRNA expression was increased in concanavalin A- and interferon γ-activated D.10 cells. The 33.5-kDa-Ubi-L adduct was expressed in spleen but not in testis, even though Bcl-G protein was highly expressed in this tissue. The antisense oligonucleotide to Bcl-G significantly decreased the level of the Ubi-L-adduct formation in concanavalin A-activated D.10 cells and the proliferative response of the D.10 cells. These results suggest that the posttranslational modification of Bcl-G by Ubi-L might be implicated in T cell activation.
|
Report
(3 results)
Research Products
(10 results)
