Mitocohdrial structure and function analysis of Ogura-type of CMS obtained in the progeny derived from interspecific chimera
| Project/Area Number |
15580004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | National University Corporation Tokyo University of Agriculture and Technology |
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Principal Investigator |
HIRATA Yutaka Tokyo University of Agriculture and Technology, Institute of Symbiotic Science and Technology, Associate Professor, 大学院・共生科学技術研究部, 助教授 (50113866)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Artificill-synthesized chimera / Oruta type of CMS / Mitochondrion / Heteropasmy / Chloroplast genome / Orf138 / matK / Hybrid breeding / 人為合性キメラ / キメラ / アブラナ科 / 細胞質雄性不稔 / 接木変異 / オグラ型細胞質 / 細胞融合 |
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| Research Abstract |
Results described below could be obtained, such that new control mechanism of CMS induction may obtain by the present experiment..
1. Three genome molecules were found and made clear the structures. The contents were published.
2. The induction mechanism is not coming from mt genome recombination but heteroplasmic genome existence and the shift among those at stoichiometric shift..
3. The CMS induction and stoichiometric shifts were induced both by chimera synthesis and protoplast fusion processes.
4. These inductive Ogura-type of CMS included base changes and minor structural changes. The facts suggested the new CMS induction possibility.
5. In the present study, mt genome and the related molecule changes were mainly shown. In addition, the homologous genome changes, especially heteroplasmic molecule (genome) existence and the shift with CMS occurred in chloroplast genome, suggesting whole control cytoplasmic genomes with nuclear genome. New research on the systematic genome change must be studied.
6. Restoration ability has not been found in B.rapa and B.oleracea. That disrupted the mechanism research and application. One urgent necessity exists in the Rf gene introduction for the control Ogura-CMS by using cell fusion or intergeneric cross.
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Report
(4 results)
Research Products
(27 results)
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[Publications] Zhang, MF, Chen LP, Wang, BL, Yang, JH, Chen, Zj, Hirata, Y.: "Characterization of atpA and orf220 genes distinctively present in a cytopalmis male-sterile line of tuber mustard."Journal of Horticultural Science and Biotechnology. 78・6. 837-841 (2003)
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