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Genetic analysis of heterosis and fertility in indica-japoinica hybrids in rice (Oryza sativa)

Research Project

Project/Area Number15580008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionNihon University

Principal Investigator

IKEHASHI Hiroshi  Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (50193222)

Co-Investigator(Kenkyū-buntansha) NOMURA Kazunari  Nihon University, College of Bioresource Sciences, Professor, 生物資源科学部, 教授 (90139048)
SHISHIDO Rieko  Nihon University, College of Bioresource Sciences, Lecturer, 生物資源科学部, 講師 (90307819)
Project Period (FY) 2003 – 2005
Project Status Completed(Fiscal Year 2005)
Budget Amount *help
¥2,900,000 (Direct Cost : ¥2,900,000)
Fiscal Year 2005 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 2004 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 2003 : ¥1,500,000 (Direct Cost : ¥1,500,000)
Keywordsheterosis / QTL / intersubspesific hybrid / over dominance / seedling traits / senescence / hybrid rice
Research Abstract

Since fertile hybrids were obtained between indica and japonica rice, utilization of the enhanced heterosis has been attempted in the inter-subspecific hybrid But the genetic basis for the heterosis has not been adequately analyzed. To specifically compare the effect of heterozygotes and homozygotes at each quantitative trait locus (QTL) in indica-japonica hybrids, two backcrossed populations(BC_1F_1), indica/japonica//japonica and indica/japonica//indica, were produced and mapped with a set of simple sequence repeat (SSR) markers. Several QTLs for grain shape traits, seedling characteristics and leaf senescence were found in the BC_1F_1orBC_1F_2lines with a LOD score of 2.5 as threshold. The effects of genotypes on respective traits were analyzed at these QTLs to analyze genetic basis of the heterosis.
QTLs were detected by comparing two marker genotypes (AA and Aa, or Aa and aa) or their progeny lines (BC_1F_2) in each of the backcrossed populations. The genotypes that were determined to be heterozygous in the BC_1F_1 lines might therefore have contained three genotypes in the BC_1F_2 lines with the predicted frequencies : (1/4AA+1/2Aa+1/4aa). No QTL was identified at which the heterozygotes (Aa) showed a higher value than the homozygotes (AA or aa) in the two-paired populations. This provides evidence that the two populations gave consistent phenotypic values in the measured traits following a linear order of genetic effects : AA>Aa>aa or AA<Aa<aa. These findings also suggest that no over-dominance (Aa>AA) was present.
The examination of genotypic effect at each QTL in this study revealed that positive effects were contributed either by indica or japonica parents. In the F_1 genotype, the cumulative partial dominance effects of such heterozygotes are considered to give better performance than the parents.

Report

(4results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report

Research Products

(22results)

All 2006 2005 2004 2003 Other

All Journal Article Publications

  • [Journal Article] Factors controlling seed germinability of rat's tail radish (Raphanus sativus L.) cv.Pakki-hood at a low temperature.2006

    • Author(s)
      Nomura, K., Uesugi, T., Tateishi, A., Shishido, R., Yoneda, K.
    • Journal Title

      Jpn.J.Trop.Agr. 50(1)

      Pages : 29-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of genetic diversity of wild rice (Oryza rufipogon Griff.) in Myanmar using simple sequence repeats (SSRs)2006

    • Author(s)
      Shishido, R., Kikuchi, M., Nomura, K., Ikehashi, H.
    • Journal Title

      Genetic Resources and Crop Evolution 53

      Pages : 179-186

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-Based Analysis of Heterosis for Grain Shape Traits and Seedling Characteristics in an indica-japonica Hybrid in Rice (Oryza sativa L)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Breeding Science 55

      Pages : 41-48

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-based Analysis of Leaf Senescence in an indica/japonica Hybrid in Rice (Oryza sativa L)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Theoretical Applied Genetics 110

      Pages : 1226-1235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Survey of genetic loci for indica/japonica heterosis in rice with SSR makers.4 Confirmation of QTLs for leaf senscence in following generations.2005

    • Author(s)
      T.Shimazaki, K.Watanabe, A.F.Abdelkhalik, R.Shishido, K.Nomura, H.Ikehashi
    • Journal Title

      10^<th> international Congress of SABRAO August 22-23, 2005 Tsukuda, Japan Abstracts

      Pages : G-3

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-Based Analysis of Heterosis for Grain Shape Traits and Seedling Characteristics in an indica-japonica Hybrid in Rice (Oryza sativa L.)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido*, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Breeding Science 55

      Pages : 41-48

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-based Analysis of Leaf Senescence in an indica/japonica Hybrid in Rice (Oryza sativa L.)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Theor.Appl.Genet. 110

      Pages : 1226-1235

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Evaluation of F_2 and F_3 plants introgressed with QTLs for clubroot resistance in cabbage developed by using SCAR markers.2005

    • Author(s)
      Nomura, K., Minegishi, Y., Kimizuka-Takagi, C., Fujioka, T., Moriguchi K., Shishisdo, R., Ikehashi, H.
    • Journal Title

      Plant Breeding 124

      Pages : 371-375

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-Based Analysis of Heterosis for Grain Shape Traits and Seedling Characteristics in an indica-japonica Hybrid in Rice (Oryza sativa L.)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Breeding Science 55

      Pages : 41-48

    • Related Report
      2005 Annual Research Report
  • [Journal Article] QTL-based Analysis of Leaf Senescence in an indica/japonica Hybrid in Rice (Oryza sativa L.)2005

    • Author(s)
      Amr Farouk Abdelkhalik, Rieko Shishido, Kazunari Nomura, Hiroshi Ikehashi
    • Journal Title

      Theoretical Applied Genetics 110

      Pages : 1226-1235

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Survey of genetic loci for indica/japonica heterosis in rice with SSR makers.4 Confirmation of QTLs for leaf senescence in following generations.2005

    • Author(s)
      T.Shimazaki, K.Watanabe, A.F.Abdelkhalik, R.Shishido, K.Nomura, H.Ikehashi
    • Journal Title

      10^<th> international Congress of SABRAO August 22-23, 2005 Tsukuba, Japan Abstracts

      Pages : G-3

    • Related Report
      2005 Annual Research Report
  • [Journal Article] QTL-based analysis of heterosis for grain shape traits and seedling characteristics in an indica-japonica hybrid in rice (Oryza sativa L.)2005

    • Author(s)
      Amr, F.A., R.Shishido, K.Nomura, H.Ikehashi
    • Journal Title

      Breeding Science 55

      Pages : 41-48

    • Related Report
      2004 Annual Research Report
  • [Journal Article] QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.)2005

    • Author(s)
      Amr F.A., R.Shishido, K.Nomura, H.Ikehashi
    • Journal Title

      Theoretical Applied Genetics 110

    • Related Report
      2004 Annual Research Report
  • [Journal Article] SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索3.老化関連遺伝子座について2004

    • Author(s)
      Amr Farouk Abdelkhalik, 島崎高嘉, 高島伸知, 長島雅和, 宍戸理恵子, 野村和成, 池橋宏
    • Journal Title

      育種学研究 第6巻(別冊1)

      Pages : 235

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] QTL-based analysis of leaf senescence in an indica-japonica rice hybrid (Oryza sativa L.)2004

    • Author(s)
      Abdelkhalik, A., R.Shishisdo, K.Nomura, H.Ikehashi
    • Journal Title

      World Rice Research Conference 5-7 November 2004 Abstract

      Pages : 213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索1.方法および幼苗形質その他について2003

    • Author(s)
      Amr Farouk Abdelkhalik, 小山幸久, 宍戸理恵子, 野村和成, 池橋宏
    • Journal Title

      育種学研究 第5巻(別冊1)

      Pages : 75

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索2.粒型および粒種について2003

    • Author(s)
      Amr Farouk Abdelkhalik, 宍戸理恵子, 野村和成, 池橋宏
    • Journal Title

      育種学研究 第5巻(別冊2)

      Pages : 213

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Isozyme variation in native varieties of Chinese kale (Brassica oleracea var. alboglabra) in Taiwan.2003

    • Author(s)
      Nomura, K., Kobayashi, M., Shishisdo, R., Kuo Jiun-Yih., Yoneda, K., Ikehashi, H.
    • Journal Title

      Jpn.J.Trop.Agr. 47

      Pages : 266-272

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索1.方法および幼苗形質その他ニツイテ2003

    • Author(s)
      Amr F.A., 小山幸久, 宍戸理恵子, 野村和也, 池橋 宏
    • Journal Title

      育種学研究 5巻別冊1

      Pages : 75

    • Related Report
      2004 Annual Research Report
  • [Journal Article] SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索2.粒型および粒重について2003

    • Author(s)
      Amr F.A., 宍戸理恵子, 野村和也, 池橋 宏
    • Journal Title

      育種学研究 5巻別冊2

      Pages : 213

    • Related Report
      2004 Annual Research Report
  • [Publications] Amr F.A., 小山幸久, 宍戸理恵子, 野村和也, 池橋 宏: "SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索1.方法および幼苗形質その他ニツイテ"育種学研究. 5巻別冊1. 75-75 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Amr F.A., 宍戸理恵子, 野村和也, 池橋 宏: "SSRマーカーによるイネ日印雑種におけるヘテロシス関連遺伝子座の探索2.粒型および粒種について"育種学研究. 5巻別冊2. 213-213 (2003)

    • Related Report
      2003 Annual Research Report

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Published : 2003-04-01   Modified : 2016-04-21  

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