| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2004: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Research Abstract |
To examine the possibility that movement proteins(MPs) of plant viruses regulate gene expression of host genes, an analysis system was developed using a putative transcriptional coactivator, named NtMBF1,which is a multi protein bridging factor 1 homolog derived from Nicotiana tabacum and binds to the MP derived from Tomato mosaic virus(ToMV). For the assay, six kinds of plasmids were constructed ; (1)a reporter plasmid which expresses beta-glucronidase(GUS) derived from Escherichia coli under control of an ethylene responsive element(ERE)-containing promoter, (2)the first effecter plasmid which expresses a tobacco transcriptional activator NtERF2, which recognize ERE, under control of the 35S promoter derived from Cauliflower mosaic virus(CaMV), (3)the second effecter plasmid which expresses NtMBF1 under control of the CaMV 35S promoter, (4)the third effecter plasmid which expresses ToMV MP under control of the CaMV 35S promoter, (5)a control plasmid which expresses green fluorescent protein under control of the CaMV 35S promoter as an internal control, (6)a control plasmid which expresses GUS under control of the CaMV 35S promoter as a control for constitutive reporter expression. These plasmids were combined differently, introduced into protoplasts prepared from tobacco BY-2 cell culture and accumulation of GUS and GFP were analyzed. The results suggest that ToMV MP would regulate expression of the host genes. This study made it more interesting to examine in the future whether similar results will be obtained not only in cultured cells but also in host plants.
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