Analysis and proposal of new aluminum resistanece strategy by developing of model technique using artificial cell membrane
Grant-in-Aid for Scientific Research (C)
|Allocation Type||Single-year Grants|
Plant nutrition/Soil science
|Research Institution||Yamagata University|
WAGATSUMA Tadao Yamagata University, Faculty of Agriculture, Professor, 農学部, 教授 (70007079)
|Project Period (FY)
2003 – 2004
Completed(Fiscal Year 2004)
|Budget Amount *help
¥3,300,000 (Direct Cost : ¥3,300,000)
Fiscal Year 2004 : ¥1,400,000 (Direct Cost : ¥1,400,000)
Fiscal Year 2003 : ¥1,900,000 (Direct Cost : ¥1,900,000)
|Keywords||aluminum resistance / plasma membrane lipid / membrane permeability / free sterol / glucocerebroside / flavonoid / ジベレリン突然変異体 / ブラシノライド突然変異体 / ナイロンカプセル / 透過性 / 有機酸放出|
The objectives have almost been completed, and finally a new aluminum resistance strategy could be proposed. I acknowledge the excellent judgement on the adoption of my research proposal. The points of my research are as follows.
(1)Development of new and simple technique for isolation of plasma mebrane(PM)
Protoplasts isolated from root-tips were adhered on polylysine coated glass plates, and washed with buffer solution. The adhered residual membranes were found to be almost free from other organelle membranes. Finally, I could develop a new simple technique for isolation of PM as compared with conventional liquid two polymer phase partitioning technique.
(2)Establishment of permeability experiment system using synthesized nylon capsule coated with PM lipid
Nylon-2, 8 capsules were synthesized and the micropores in their membranes were coated with lipid bilayer. This experimental system was superior to the converntional liposome system. Organic acid anion release from roots was not signif
icantly different between two lines of triticale, but on the other hand, PM lipid which is qualitatively rich in free sterols and glucocerebrosides of Al-resistant triticale line was less permeable than that of Al-sensitive line in Al medium.
(3)Association of methylene blue(MB)stainability of protoplasts isolated from root-tips with Al resistance
Significant negative correlation was recognized between Al resistance and MB stainability of protoplasts among many plant species, cultivars and lines. This suggests that Al resistant plants have their root-tips with lower negativity and stronger PM.
(4)Comparison of Al resistances among brassinosteroid(BR) mutants and gibberellin (GA) mutants of pea
Among several BR mutants and GA mutants of pea, Ih mutant was most sensitive to high Al conditions. This highest sensitivity was correlated with the greatest permeability of PM, but not with OA release from roots. This may be associated with the abnormal accumulation of free sterols in PM lipid layer.
(5)Hyper Al resistance of signalgrass
Permeability of root-tip PM for signalgrass was considerably low. High deposition of flavonoids around the peripheral area of root-tip cells was newly found ; this can also contribute to the least permeability of signalgrass PM. Less
Research Products (18results)