Gene expression analysis of intestinal intraepithelial lymphocytes derived from mice fed a food antigen or functional food factors.
| Project/Area Number |
15580101
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TOTSUKA Mamoru The University of Tokyo, Graduate School of Agricultural and Life Sciences, Visiting Associate Professor, 大学院・農学生命科学研究科, 客員助教授 (70227601)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Intestinal intraepithelial lymphocyte / DNA microarray analysis / Interleukin-10 / Regulatory T cell / Food antigen uptake / Gut immune system / CD$^+ T cell / 脾臓T細胞 / 遺伝子発現の網羅的解析 / グランザイム |
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| Research Abstract |
We focused on intestinal intraepithelial lymphocytes (IEL), which are a kind of T cells residing in intestinal epithelia, and the gene expression in IEL derived from mice fed a food antigen was analyzed. When ovalbumin-specific T-cell receptor transgenic mice (DO11.10 mice) were fed a diet containing egg white proteins (EW-diet) ad libitum for 3 days, increase in the number of CD4^+ IEL among whole IEL and alteration of cell-surface molecule expression on IEL were observed. DNA microarray analysis of IEL derived from mice fed the EW-diet showed the increased expression of 98 genes and decreased expression of 24 genes. Genes with increased expression contained cell-cycle related genes and immune-related genes including interleukin-10 (IL-10). IEL derived from the EW-fed mice showed increased secretion of IL-10 compared to the control IEL. IL-10 gene expression was significantly observed in CD4^+ IEL subset without in vitro stimulation, and the expression level was higher than CD4^+ T cells from other gut-associated lymphoid tissues. Since IL-10 gene expression per CD4^+ IEL was not increased by feeding the EW-diet, the increased cell number of CD4^+ IEL subsets was suggested to be responsible for the increased gene expression of IL-10 in IEL from the EW-fed mice. When the gene expression profiles were compared among IEL subsets, the expression of granzyme A was shown to be much less in CD4^+ IEL than in other IEL subsets. Since IL-10 is a cytokine important for the immune-suppressive function of a kind of regulatory T cells, CD4^+ IEL may have a function in regulating the immune responses in intestinal mucosa, and the regulatory function of IEL would be increased by feeding a food antigen.
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Report
(3 results)
Research Products
(27 results)
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[Journal Article] Antigen feeding enhances frequency and antigen-specific proliferation ability of intraepithelial CD4^+ T cells in αβ T cell receptor transgenic mice.2003
Author(s)
Masao Goto, Satoshi Hachimura, Akio Ametani, Takehito Sato, Yoshihiro Kumagai, Sonoko Habu, Mamoru Totsuka, Hiromichi Ishikawa, Shuichi Kaminogawa.
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Journal Title
Bioscience, Biotechnology, and Biochemistry 67
Pages: 1223-1229
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Dendritic cells from spleen, mesenteric lymph node and Peyer's patch can induce both production of IL-4 and IFN-γ of naive CD4^+ T cells in their primary culture, depending on antigen doses.2003
Author(s)
Masanobu Hibi, Satoshi Hachimura, Wataru Ise, Ayuko Sato, Tadashi Yoshida, Tsuyoshi Takayama, Kastumi Sasaki, Takashi Senga, Shuichi Hashizume, Mamoru Totsuka, Shuichi Kaminogawa.
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Journal Title
Cytotechnology 43
Pages: 49-55
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Book] 食品の科学2005
Author(s)
戸塚 護(分担執筆)
Total Pages
216
Publisher
東京化学同人
Description
「研究成果報告書概要(和文)」より
Related Report
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