| Co-Investigator(Kenkyū-buntansha) |
HIRAI Hirofumi Shizuoka University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70322138)
KAWAI Shigo Shizuoka University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70192549)
|
|---|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
|
|---|
| Research Abstract |
Considerable concern has recently been expressed over the possibility that endocrine disruptors having estrogenic activities may adversely affect reproduction in wildlife and humans. Therefore, endocrine disruptors, bisphenol A (BPA), nonylphenol (NP), 17β-estradiol (E_2), ethinylestradiol (EE_2), and genistein (GE), were treated with white rot fungi under ligninolytic condition with low nitrogen and high-carbon culture medium. Marked decreases in BPA, NP, E_2, and EE_2 were obtained by the treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, thus suggesting that the disappearance of these comopunds is related to ligninolytic enzymes produced extracellularly by white rot fungi. Therefore, BPA, NP, E_2, and EE_2 were treated with MnP and lactase.
HPLC analysis demonstrated that all compounds disappeared almost completely in the reaction mixture after 1 h of treatment with MnP or lactase. Using the yeast two-hybrid assay system, it was also confirmed that these enzymatic treatments completely removed the estrogenic activities of BPA, NP, E_2, and EE_2 within a 12-h treatment with MnP or laccase. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activities of BPA, NP, E_2, and EE_2.
|
