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Elucidation of the substrate specificity mechanism of phospholipase A2 from marine organisms

Research Project

Project/Area Number 15580175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionHokkaido University

Principal Investigator

KISHIMURA Hideki  Hokkaido University, Faculty of Fisheries Sciences, Associate Professor, 大学院水産科学研究院, 助教授 (50204855)

Co-Investigator(Kenkyū-buntansha) OJIMA Takao  Hokkaido University, Faculty of Fisheries Sciences, Professor, 大学院水産科学研究院, 教授 (30160865)
Project Period (FY) 2003 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
KeywordsMarine invertebrate / Echinoderm / Asterina pectinifera / Phospholipase A2 / Mutant / Substrate specificity / Specific activity / Pancreatic loop / ホスホリパーゼA2 / Pancreaticループ / ヤツデヒトデ / ホスホリパゼA2 / pancreatic ループ / タコヒトデ / pancreaticループ / 遺伝子工学 / 基質極性基特異性
Research Abstract

1.The specific activities of the mutants that were introduced Lys residue in pancreatic loop region for phosphatidylcholine were lower than that of native phospholipase A2 (PLA2) from the pyloric ceca of the starfish.4sterinapectinira, similarly to that of porcine pancreatic PLA2. It was suggested that the charge and structure of pancreatic loop region of the starfish PLA2 might carry out important role on polar-group specificity.
2.The PLA2 isozyme from the starfish A. pectinifera and the PLA2s from other starfish species well hydrolyzed phosphatidylcholine similar to the PLA2 from the starfish A. pectinifera It was suggested that the polar-group specificity of the starfish PLA2 might common property with the PLA2 from Asteroid.
3.The PLA2 activity of the starfish A. pectinifera was very high in every seasons and areas, whereas, the PLA2 activity of the starfish juvenile was extremely lower than that of the mature starfish. It was suggested that the very high activity

Report

(5 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (18 results)

All 2007 2005 2004 2003 Other

All Journal Article (17 results) Publications (1 results)

  • [Journal Article] Characteristics of phospholipase A2 of the starfish Asterina pectinifera2007

    • Author(s)
      Hideki Kishimura
    • Journal Title

      Enzyme and Microbial Technology 40

      Pages: 461-465

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of phospholipase A2 mutant of the starfish Asterina pectinifera2007

    • Author(s)
      H.Kishimura
    • Journal Title

      Enzyme and Microbial Technology 40

      Pages: 461-465

    • NAID

      120000966042

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characteristics of phospholipase A2 mutant of the starfish Asterina pectinifera2007

    • Author(s)
      II.Kishimura, S.Ando
    • Journal Title

      Enzyme and Microbial Technology 40

      Pages: 461-465

    • NAID

      120000966042

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasterias acutispina and Plazaster borealis2005

    • Author(s)
      Hideki Kishimura
    • Journal Title

      Food chemistry 92

      Pages: 407-411

    • NAID

      120000954517

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Characterization of phospholipase A2 from the pyloric ceca of two species of starfish, Coscinasteria acutispina and Plazaster borealis2005

    • Author(s)
      H.Kishimura
    • Journal Title

      Food Chemistry 92

      Pages: 407-411

    • NAID

      120000954517

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Properties of phospholipase A2 from the pyloric ceca of the starfish Coscinasterias acutispina and Plazaster borealis.2005

    • Author(s)
      Hideki Kishimura
    • Journal Title

      Food Chemistry 92・3

      Pages: 407-411

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イトマキヒトデ幽門盲のう由来ホスホリパーゼA2アイソザイムの基質特異性2004

    • Author(s)
      岸村 栄毅
    • Journal Title

      日本水産学会誌 70・1

      Pages: 54-59

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary 2004 Annual Research Report
  • [Journal Article] Purification and properties of phospholipase A2 isozymes from the pyloric ceca of the starfish Asterina pectinifera2004

    • Author(s)
      Hideki Kishimura
    • Journal Title

      Journal of Food Biochemistry 28

      Pages: 181-194

    • NAID

      120000953988

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Partial purification and characteristics of phospholipase A2 from pyloric ceca of the starfish Plazaster borealis2004

    • Author(s)
      Noriki Koyama
    • Journal Title

      Bulletin of the Faculty of Fisheries Hokkaido University 55・1

      Pages: 11-16

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Substrate specificity of phospholipase A2 isozyme from the pyloric ceca of the starfish Astarinz pectinifera2004

    • Author(s)
      II.Kishimura
    • Journal Title

      Nippon Suisan Gakkaishi 70(1)

      Pages: 54-59

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Purification and properties of phospholipase A2 isozymes from pyloric ceca of the starfish Asterinz pectinifera2004

    • Author(s)
      H.Kishimura
    • Journal Title

      Journal of Food Biochemistry 28

      Pages: 181-194

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Partial purification and characteristics of phospholipase A2 from pyloric ceca of starfish Plazastel borealis2004

    • Author(s)
      N.Koyama
    • Journal Title

      Bulletin of Fisheries Sciences Hokkaido University 55(1)

      Pages: 11-16

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Purification and properties of phospholipase A2 isozymes from pyloric ceca of the starfish (Asterina pectinifera)2004

    • Author(s)
      Hideki Kishimura
    • Journal Title

      Journal of Food Biochemistry 28・3

      Pages: 181-194

    • NAID

      120000953988

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 棘皮動物ホスホリパーゼA2の構造と機能2004

    • Author(s)
      岸村 栄毅
    • Journal Title

      日本水産学会誌 70・5

      Pages: 770-771

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Partial purification and characteristics of phospholipase A2 From pyloric ceca of starfish Plazaster borealis2004

    • Author(s)
      Noriki Koyama
    • Journal Title

      Bulletin of Fisheries Sciences, Hokkaido University 55・1

      Pages: 11-16

    • Related Report
      2004 Annual Research Report
  • [Journal Article] イトマキヒトデホスホリパーゼA2変異体の基質極性基特異性2003

    • Author(s)
      岸村 栄毅
    • Journal Title

      日本水産学会誌 69・6

      Pages: 968-974

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Polar group specificity of starfish phospholipase A2 mutant.2003

    • Author(s)
      H.Kishimura
    • Journal Title

      1Nippon Suisan Gakkaishi 69(6)

      Pages: 968-974

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Publications] 岸村 栄毅: "イトマキヒトデホスホリパーゼA2変異体の基質極性基特異性"日本水産学会誌. 69・6. 968-974 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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