Redundant function of Sox17 and Sox18 in mouse angiogenesis
| Project/Area Number |
15580254
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KANAI Yoshiakira The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (30260326)
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| Co-Investigator(Kenkyū-buntansha) |
YONEKAWA Hiromichi The Tokyo Metropolitan Institute of Medical Science, Vice-Director (Researcher), 実験動物研究部門, 副所長(研究職) (30142110)
HAYASHI Yoshihiro The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (90092303)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Sox17 / Sox18 / angiogenesis / liver / kidney / Vcam-1 / gut endoderm / mouse / Sox07 / 欠陥形成 |
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| Research Abstract |
The Sry-related HMG box transcription factor gene, Sox18, a member of the Sox subgroup F (Sox7, Sox17 and Sox18), was previously shown to have a crucial role in proper vascular development in both mouse and human. Here we show a redundant function of Sox17, another member essential for gut endoderm formation, in proper vascular formation during mouse postnatal development. In order to examine a possible redundant function of Sox17 and Sox18, we have generated the Sox17^<+/->/Sox18^<-/-> mutant mice, and compared their phenotypes with those of Sox17^<+/->/Sox18^<-/-> or Sox17^<+/->/Sox18^<+/-> mutant mice. As a result, both Sox17^<+/+>/Sox18^<-/-> and Sox17^<+/->/Sox18^<+/->mutants were viable and fertile and do not display any gross anatomical or physical abnormalities. In contrast, Sox17^<+/->/Sox18^<-1->mutant clearly showed defective angiogenesis during 4 to 14 dpp, which most likely caused postnatal lethality in approximately half of the Sox17^<+/->/Sox18^<-/-> mice. The earliest recognizable defect is reduced blood vessels in the liver and kidney at around 4 dpp, which may promote necrotic cell death of both liver hepatic and kidney urinary tubule epithelial cells during 7-14 dpp. Therefore, these findings strongly indicate that Sox17 and Sox18 are jointly required for proper angiogenesis of several organs in the postnatal development, highlighting the idea that all three Sox subgroup F members are cooperatively involved in the mammalian vascular development.
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Report
(3 results)
Research Products
(16 results)
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[Journal Article] Influence on spatiotemporal patterns of a male-specific Sox9 activation by ectopic Sry Expression during early phases of testis differentiation in mice.2005
Author(s)
Kidokoro, T., Matoba S., Hiramatsu, R., Fujisawa, M., Kanai-Azuma, M., Taya, C., Kurohmaru, M., Kawakami, H., Hayashi, Y., Kanai Y., Yonekawa, H.
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Journal Title
Dev.Biol. 278
Pages: 511-525
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Conditional activation of RhoA suppresses the epithelial to mesenchymal transition at the primitive streak during mouse gastrulation.2004
Author(s)
Fuse, T., Kanai, Y., Kanai-Azuma, M., Suzuki, M., Nakamura, K., Mori, H., Hayashi, Y., Mishina, M.
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Journal Title
Biochem.Biophys.Res.Commun. 318
Pages: 665-672
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A close correlation in the expression patterns of Af-6 and Usp9x in Sertoli and granulosa cells of mouse testis and ovary.2004
Author(s)
Sato, T., Kanai, Y., Noma, T., Kanai-Azuma, M., Taya, S., Matsui, T., Ishii, M., Kawakami, H., Kurohmaru, K., Kaibuchi, K., Wood, S.A., Hayashi, Y.
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Journal Title
Reproduction 128
Pages: 583-594
Description
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[Journal Article] Regionally distinct potencies of mouse XY genital ridge to initiate testis differentiation dependent on anteroposterior axis.2003
Author(s)
Hiramatsu, R., Kanai.Y., Mizukami, T., Ishii, M., Matoba, S., Kanai-Azuma, M., Kurohmaru, M., Kawakami, H., Hayashi, Y.
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Journal Title
Dev.Dyn. 228
Pages: 247-253
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Cofilin Phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.2003
Author(s)
Nakano, K., Kanai-Azuma, M., Kanai, Y., Moriyama, T., Yazaki, K., Hayashi, Y., Kitamura, T.
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Journal Title
Exp.Cell Res. 287
Pages: 219-227
Description
「研究成果報告書概要(欧文)」より
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