A role of lymphocyte-derived cytokines in the maintenance and regeneration of sympathetic neurons
| Project/Area Number |
15580263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KANNAN Yukiko Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Visiting Researcher, 生命環境科学研究科, 客員研究員 (80264810)
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| Co-Investigator(Kenkyū-buntansha) |
MORIYAMA Mitsuaki Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Associate Professor, 生命環境科学研究科, 助教授 (20275283)
KUWAMURA Mitsuru Osaka Prefecture University, Graduate School of Life & Environmental Sciences, Assistant Professor, 生命環境科学研究科, 講師 (20244668)
中村 洋一 大阪府立大学, 生命環境科学研究科, 教授 (90180413)
藤本 由香 大阪府立大学, 生命環境科学研究科, 助手 (40405361)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | SCID / T lymphocytes / IL-3 / Spleen / Sympathetic neurons / 6-OHDA / Tyrosine hydroxylase / Interleukin 3 / tyrosine hydroxylase / spleen / sympathetic nerves / IL-1 receptor antagonist / lymphocytes |
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| Research Abstract |
Immunohistochemical examination revealed that the spleens of immunodeficient (SCID) mice that lack T and B cells had significantly fewer tyrosine hydroxylase (TH)-positive fibers in their periarteriolar lymphatic sheaths (PALS) than their wild type C.B-17 mice. Two weeks after SCID mice were injected with C.B-17 splenic T cells (10^5-10^6 cells), their TH-positive fiber staining increased in the PALS ; there were no significant changes in TH-positive innervation following the injection of SCID mice with C.b-17 cells. T and B cell reconstitution in treated SCID mice was confirmed immunohistochemically using anti-CD3 and anti-IgG antibodies, respectively. IL-3 levels, measured by ELISA, increased significantly in SCID mice following T cell reconstitution. The administration of anti-IL-3 antibody blocked the T cell-induced increase in innervation in the PALS. Anti-Il-3 treatment also inhibited the regeneration of splenic sympathetic neurons in C.B-17 mice after they were chemically sympathetomized with 6-hydroxydopamine (6-OHDA). Our results suggest that T cells play a role in regulating the sympathetic innervation of the spleen, this effect appeared to be mediated at least in part by IL-3.
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Report
(4 results)
Research Products
(18 results)
